Abstract
Tobacco tapetum-specific promoter pTA29 is widely used to create male sterile lines and to study pollen development in various plants. However, the pTA29 expression characteristics in cotton is still not elucidated. To determine the expression characteristics of pTA29 promoter in cotton, gus gene was fused downstream to pTA29, transformed into cotton. It was demon-strated that 10% alcohol suppressed the intrinsic GUS-like activity in cotton anther. GUS staining with modified solution showed that the GUS activity in the pTA29:gus transformants mainly existed in anthers with two peaks in the floral buds of 6 and 15 mm, while no GUS activity was detected in roots, stems, ovules, petals and bracts. Inconsistent with that in tobacco, the GUS activity was also detected in the leaf trichomes and pollens in pTA29:gus transformants. Real-time RT-PCR analysis indicated that the gus transcripts accumulated in a same pattern with the GUS activity. These results suggest that the tobacco tapetum-specific promoter pTA29 can drive the downstream genes to express preferentially in anthers of cotton. The application of pTA29 in cotton male sterility engineering was discussed.
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Claims 6
Claim · model-written summary
Ten percent alcohol suppressed intrinsic GUS-like activity in cotton anthers.
- Taxon
- cotton
Gossypium hirsutume0 - Organ / stage
- anthere0
- Treatment
- 10% alcohole0
- Outcome
- intrinsic GUS-like activitye0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“10% alcohol suppressed the intrinsic GUS-like activity in cotton anther.”
Claim · model-written summary
pTA29:gus GUS activity mainly occurred in anthers, peaking at 6 and 15 mm floral buds.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- antherse0
- Gene
- pTA29:guse0
- Outcome
- GUS activitye0
- Value
- 6 and 15 mme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUS activity in the pTA29:gus transformants mainly existed in anthers with two peaks in the floral buds of 6 and 15 mm”
t0“Cotton”
Claim · model-written summary
GUS activity was absent from roots, stems, ovules, petals and bracts.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- roots, stems, ovules, petals and bractse0
- Outcome
- no GUS activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no GUS activity was detected in roots, stems, ovules, petals and bracts.”
t0“Cotton”
Claim · model-written summary
Unlike tobacco, pTA29:gus cotton transformants showed GUS activity in leaf trichomes and pollen.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- leaf trichomes and pollense0
- Gene
- pTA29:guse0
- Outcome
- GUS activitye0
- Comparator
- tobaccoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inconsistent with that in tobacco, the GUS activity was also detected in the leaf trichomes and pollens in pTA29:gus transformants.”
t0“Cotton”
Claim · model-written summary
gus transcript accumulation followed the same pattern as GUS activity.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- guse0
- Outcome
- gus transcriptse0
- Comparator
- GUS activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gus transcripts accumulated in a same pattern with the GUS activity.”
t0“Cotton”
Claim · model-written summary
The authors infer pTA29 preferentially drives expression in cotton anthers.
- Taxon
- cottonGossypium hirsutume0
- Gene
- pTA29e0
- Outcome
- express preferentially in antherse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco tapetum-specific promoter pTA29 can drive the downstream genes to express preferentially in anthers of cotton.”