Abstract
Hydrogen sulfide (H 2 S) induces stomatal closure in Arabidopsis thaliana, hydrogen peroxide (H2O2 ) plays an important part in this procedure. In this paper, Arabidopsis wide type and its α subunit deficient mutants (Atgpa1-3 and Atgpa1-4) as well as β subunit deficient mutants (Atagb1-1 and Atagb1-2) were used as materials, the effects of G-protein on H2S-induced stomatal closure and the relationship between G-protein and H2O2 were investigated. The results showed that H2S induced stomatal closure as well as the expression of Gprotein α subunit (GPA) and β subunit (AGB) gens in leaves of Arabidopsis wild type, but had no significant effects on stomatal movement in Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutants. GPA activator cholera toxin (CTX) improved H2S-induced stomatal closure, but its inhibitors pertussis toxin (PTX) showed the opposite effect. These results manifested that G-protein was an signaling intermediate in H2S-induced stomatal closure. H2S induced increase in H2O2 content in leaves and guard cells of A. thaliana wide type, but not in Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutants. CTX enhanced H2O2 content in leaves and guard cells of Arabidopsis, but its inhibitor PTX was opposite. From these data it could be deduced that G-protein acts upstream of H2O2 in mediating H2S-induce stomatal closure in Arabidopsis.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher.
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
H2S induced stomatal closure in wild type Arabidopsis thaliana.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- wild typee1
- Organ / stage
- leavese1
- Treatment
- H2Se1
- Outcome
- stomatal closuree1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydrogen sulfide (H 2 S) induces stomatal closure in Arabidopsis thaliana”
e1“The results showed that H2S induced stomatal closure as well as the expression of Gprotein α subunit (GPA) and β subunit (AGB) gens in leaves of Arabidopsis wild type”
Claim · model-written summary
H2S had no significant effect on stomatal movement in the Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutants, compared with wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutantse1
- Treatment
- H2Se0
- Outcome
- stomatal movemente1
- Direction
- no significant effectse1
- Comparator
- wild typee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydrogen sulfide (H 2 S) induces stomatal closure in Arabidopsis thaliana”
e1“but had no significant effects on stomatal movement in Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutants”
e2“in leaves of Arabidopsis wild type”
Claim · model-written summary
The GPA activator cholera toxin (CTX) improved H2S-induced stomatal closure.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- GPA activator cholera toxin (CTX)e3
- Outcome
- H2S-induced stomatal closuree3
- Direction
- improvede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydrogen sulfide (H 2 S) induces stomatal closure in Arabidopsis thaliana”
e3“GPA activator cholera toxin (CTX) improved H2S-induced stomatal closure, but its inhibitors pertussis toxin (PTX) showed the opposite effect”
Claim · model-written summary
The inhibitor pertussis toxin (PTX) had the opposite effect on H2S-induced stomatal closure.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- pertussis toxin (PTX)e3
- Outcome
- H2S-induced stomatal closuree3
- Direction
- opposite effecte3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydrogen sulfide (H 2 S) induces stomatal closure in Arabidopsis thaliana”
e3“GPA activator cholera toxin (CTX) improved H2S-induced stomatal closure, but its inhibitors pertussis toxin (PTX) showed the opposite effect”
Claim · model-written summary
G-protein is a signalling intermediate in H2S-induced stomatal closure.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- G-proteine4
- Outcome
- H2S-induced stomatal closuree4
- Qualifier
- signaling intermediatee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydrogen sulfide (H 2 S) induces stomatal closure in Arabidopsis thaliana”
e4“These results manifested that G-protein was an signaling intermediate in H2S-induced stomatal closure”
Claim · model-written summary
H2S induced an increase in H2O2 content in leaves and guard cells of wild type Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- wide typee5
- Organ / stage
- leaves and guard cellse5
- Treatment
- H2Se5
- Outcome
- H2O2 contente5
- Direction
- increasee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydrogen sulfide (H 2 S) induces stomatal closure in Arabidopsis thaliana”
e5“H2S induced increase in H2O2 content in leaves and guard cells of A. thaliana wide type”
Claim · model-written summary
H2S did not increase H2O2 content in the Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutantse6
- Treatment
- H2Se0
- Outcome
- H2O2 contente5
- Direction
- but not ine6
- Comparator
- wide typee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hydrogen sulfide (H 2 S) induces stomatal closure in Arabidopsis thaliana”
e5“H2S induced increase in H2O2 content in leaves and guard cells of A. thaliana wide type”
e6“but not in Atgpa1-3, Atgpa1-4, Atagb1-1 and Atagb1-2 mutants”
Claim · model-written summary
CTX enhanced H2O2 content in leaves and guard cells of Arabidopsis.
- Taxon
- ArabidopsisArabidopsise7
- Organ / stage
- leaves and guard cellse7
- Treatment
- CTXe7
- Outcome
- H2O2 contente7
- Direction
- enhancede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“CTX enhanced H2O2 content in leaves and guard cells of Arabidopsis, but its inhibitor PTX was opposite”
Claim · model-written summary
The inhibitor PTX had the opposite effect on H2O2 content.
- Taxon
- ArabidopsisArabidopsise7
- Treatment
- its inhibitor PTXe7
- Outcome
- H2O2 contente7
- Direction
- was oppositee7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“CTX enhanced H2O2 content in leaves and guard cells of Arabidopsis, but its inhibitor PTX was opposite”
Claim · model-written summary
G-protein acts upstream of H2O2 in mediating H2S-induced stomatal closure in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise7
- Gene
- G-proteine7
- Outcome
- stomatal closuree7
- Qualifier
- acts upstream of H2O2e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“From these data it could be deduced that G-protein acts upstream of H2O2 in mediating H2S-induce stomatal closure in Arabidopsis”