Paper · 2003 · ACTA AGRONOMICA SINICA

Primary Study on Identification of Corn-borer Resistance in Corn Inbred-lines Transferred with Bt Gene

Liu Zong

7
claims
18
verbatim quotes
1
citations
3
research areas
—
Not open access

Abstract

Using both inoculation of corn borer eggs in the field and PCR molecular test in laboratory, the corn borer resistance and the existence of Bt gene were identified in four corn inbred lines transferred with Bt gene. The results showed that the resistance to corn borer of the four transgenic inbred lines was increased obviously and the mean of worm tunnel length was significantly decreased by 49.71% compared with the negative controls, but the resistance level was not high as the positive control. Within each population of the four inbred lines, the resistance varied from high level of resistance to high level of sensitivity. Comparing with the negative controls, the tunnel length of the resistant plants was significantly decreased by 78.40%. DNA fragments specific for the Bt gene were detected from the four inbred lines by PCR. The inbred lines with Bt gene and high resistant to corn borer were selected. These inbred lines would facilitate breeding corn hybrid with Bt gene.

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Claims 7

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Worm tunnel length of the four Bt inbred lines was significantly decreased by 49.71% compared with negative controls.

Material
four transgenic inbred linese1
Treatment
transferred with Bt genee0
Interactor
corn borere0pest
Gene
Bt genee0
Outcome
worm tunnel lengthe1
Direction
decreasede1
Value
49.71%e1
Comparator
negative controlse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Corn Inbred-lines”

✓ located verbatim in the source · title, characters 60–77 · show in text ↑
e0

“Using both inoculation of corn borer eggs in the field and PCR molecular test in laboratory, the corn borer resistance and the existence of Bt gene were identified in four corn inbred lines transferred with Bt gene”

✓ located verbatim in the source · abstract, characters 0–214 · show in text ↑
e1

“the resistance to corn borer of the four transgenic inbred lines was increased obviously and the mean of worm tunnel length was significantly decreased by 49.71% compared with the negative controls”

✓ located verbatim in the source · abstract, characters 240–437 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Resistance to corn borer of the four transgenic inbred lines increased obviously.

Material
four transgenic inbred linese1
Treatment
transferred with Bt genee0
Interactor
corn borere0pest
Outcome
resistance to corn borere1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Corn Inbred-lines”

✓ located verbatim in the source · title, characters 60–77 · show in text ↑
e0

“Using both inoculation of corn borer eggs in the field and PCR molecular test in laboratory, the corn borer resistance and the existence of Bt gene were identified in four corn inbred lines transferred with Bt gene”

✓ located verbatim in the source · abstract, characters 0–214 · show in text ↑
e1

“the resistance to corn borer of the four transgenic inbred lines was increased obviously”

✓ located verbatim in the source · abstract, characters 240–328 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Resistance level of the Bt inbred lines was not as high as the positive control.

Treatment
transferred with Bt genee0
Interactor
corn borere0pest
Outcome
resistance levele2
Direction
not highe2
Comparator
positive controle2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Corn Inbred-lines”

✓ located verbatim in the source · title, characters 60–77 · show in text ↑
e0

“Using both inoculation of corn borer eggs in the field and PCR molecular test in laboratory, the corn borer resistance and the existence of Bt gene were identified in four corn inbred lines transferred with Bt gene”

✓ located verbatim in the source · abstract, characters 0–214 · show in text ↑
e2

“but the resistance level was not high as the positive control”

✓ located verbatim in the source · abstract, characters 439–500 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Tunnel length of resistant plants was significantly decreased by 78.40% compared with negative controls.

Material
resistant plantse4
Treatment
transferred with Bt genee0
Interactor
corn borere0pest
Outcome
tunnel lengthe4
Direction
decreasede4
Value
78.40%e4
Comparator
negative controlse4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Corn Inbred-lines”

✓ located verbatim in the source · title, characters 60–77 · show in text ↑
e0

“Using both inoculation of corn borer eggs in the field and PCR molecular test in laboratory, the corn borer resistance and the existence of Bt gene were identified in four corn inbred lines transferred with Bt gene”

✓ located verbatim in the source · abstract, characters 0–214 · show in text ↑
e4

“Comparing with the negative controls, the tunnel length of the resistant plants was significantly decreased by 78.40%”

✓ located verbatim in the source · abstract, characters 633–750 · show in text ↑
Observationobservedoriginal resultmixedmeasured

Claim · model-written summary

Resistance to corn borer varied within each inbred-line population from high resistance to high sensitivity.

Material
four inbred linese3
Outcome
the resistancee3
Qualifier
from high level of resistance to high level of sensitivitye3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Corn Inbred-lines”

✓ located verbatim in the source · title, characters 60–77 · show in text ↑
e3

“Within each population of the four inbred lines, the resistance varied from high level of resistance to high level of sensitivity”

✓ located verbatim in the source · abstract, characters 502–631 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

DNA fragments specific for the Bt gene were detected by PCR in the four inbred lines.

Material
four inbred linese5
Gene
Bt genee5
Outcome
DNA fragments specific for the Bt genee5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Corn Inbred-lines”

✓ located verbatim in the source · title, characters 60–77 · show in text ↑
e5

“DNA fragments specific for the Bt gene were detected from the four inbred lines by PCR”

✓ located verbatim in the source · abstract, characters 752–838 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Inbred lines with the Bt gene and high resistance to corn borer were selected.

Material
inbred linese6
Gene
Bt genee6
Outcome
high resistant to corn borere6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Corn Inbred-lines”

✓ located verbatim in the source · title, characters 60–77 · show in text ↑
e6

“The inbred lines with Bt gene and high resistant to corn borer were selected”

✓ located verbatim in the source · abstract, characters 840–916 · show in text ↑

transgenic · disease · Zea mays (maize)Positive and negative results

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transgenic · pest · Zea mays (maize)Positive and negative results

41 papers: 22 positive, 19 negative or no effect

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  1. no effect
  2. negative
  3. no effect

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.