Paper · 2001 · Xi'nan Nongye Daxue xuebao

APPROACHES FOR REDUCING EXPLANT BROWNING OF "CANGXILI" PEAR ( PYRUS PYRIFOLIA NAKAI) IN TISSURE CULTURE

Qiao Ni-jiao

11
claims
11
verbatim quotes
3
citations
3
research areas
—
Not open access

Abstract

The explants of Cangxili, a pear ( Pyrus pyrifolia Nakai) genotype, were found to grow well on the medium MS+1.0 mg/L BA+ 0.02 mg/L NAA. The explants were soaked in a solution containing 8-hydroxyquinoline (8-HQS) for different time duration, the treatment for 20 min gave the most satisfactory results: it prevented the browning of the explants and had no inhibiting effect of their growth, with a browning rate of 10% and a survival rate of 90%. In contrast, 8-HQS treatment for 2 hr resulted in a 100% browning and 0% survival. Survival rate varied with the season of explant collection. Highest browning rate occurred in explants collected in April. From May on, the browning rate decreased, and it was reduced to 0 in October. In antioxidant treatments, lower temperature plus 20 min 8-HQS (0.002 mg/L) or sodium benzoate (0.2 mg/L) before inoculation appeared to be optimum for reducing browning rate and improving survival of the explants.

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Claims 11

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Cangxili pear explants grew well on MS medium with BA and NAA.

Material
Cangxilie0
Treatment
MS+1.0 mg/L BA+ 0.02 mg/L NAAe0
Outcome
grow welle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Pyrus communis
Pyrus communis · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The explants of Cangxili, a pear ( Pyrus pyrifolia Nakai) genotype, were found to grow well on the medium MS+1.0 mg/L BA+ 0.02 mg/L NAA.”

✓ located verbatim in the source · abstract, characters 0–136 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

A 20-minute 8-HQS soak prevented browning, with a 10% browning rate.

Treatment
treatment for 20 mine0
Outcome
browning ratee0
Direction
preventede0
Value
10%e0

Not reportedTaxonComparator

Verbatim

e0

“the treatment for 20 min gave the most satisfactory results: it prevented the browning of the explants and had no inhibiting effect of their growth, with a browning rate of 10% and a survival rate of 90%.”

✓ located verbatim in the source · abstract, characters 243–447 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The 20-minute treatment had a 90% explant survival rate.

Treatment
treatment for 20 mine0
Outcome
survival ratee0
Value
90%e0

Not reportedTaxon

Verbatim

e0

“the treatment for 20 min gave the most satisfactory results: it prevented the browning of the explants and had no inhibiting effect of their growth, with a browning rate of 10% and a survival rate of 90%.”

✓ located verbatim in the source · abstract, characters 243–447 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

A 20-minute treatment did not inhibit explant growth.

Treatment
treatment for 20 mine0
Outcome
growthe0
Direction
no inhibiting effecte0

Not reportedTaxonComparator

Verbatim

e0

“the treatment for 20 min gave the most satisfactory results: it prevented the browning of the explants and had no inhibiting effect of their growth”

✓ located verbatim in the source · abstract, characters 243–390 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Two-hour 8-HQS treatment yielded 100% browning.

Treatment
8-HQS treatment for 2 hre0
Outcome
browninge0
Value
100%e0

Not reportedTaxon

Verbatim

e0

“8-HQS treatment for 2 hr resulted in a 100% browning and 0% survival.”

✓ located verbatim in the source · abstract, characters 461–530 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Two-hour 8-HQS treatment yielded 0% survival.

Treatment
8-HQS treatment for 2 hre0
Outcome
survivale0
Value
0%e0

Not reportedTaxon

Verbatim

e0

“8-HQS treatment for 2 hr resulted in a 100% browning and 0% survival.”

✓ located verbatim in the source · abstract, characters 461–530 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Explant survival varied with collection season.

Organ / stage
explante0
Outcome
Survival ratee0

Not reportedTaxonDirection

Verbatim

e0

“Survival rate varied with the season of explant collection.”

✓ located verbatim in the source · abstract, characters 531–590 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Explant browning was highest for April collections.

Outcome
browning ratee0
Direction
Higheste0
Qualifier
Aprile0

Not reportedTaxon

Verbatim

e0

“Highest browning rate occurred in explants collected in April.”

✓ located verbatim in the source · abstract, characters 591–653 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Browning decreased from May and reached zero in October.

Outcome
browning ratee0
Direction
decreasede0
Value
0e0
Qualifier
Octobere0

Not reportedTaxon

Verbatim

e0

“From May on, the browning rate decreased, and it was reduced to 0 in October.”

✓ located verbatim in the source · abstract, characters 654–731 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Low temperature with 20-minute 8-HQS treatment appeared optimal for reducing browning and improving survival.

Treatment
lower temperature plus 20 min 8-HQS (0.002 mg/L)e0
Outcome
reducing browning rate and improving survivale0

Not reportedTaxon

Verbatim

e0

“lower temperature plus 20 min 8-HQS (0.002 mg/L) or sodium benzoate (0.2 mg/L) before inoculation appeared to be optimum for reducing browning rate and improving survival of the explants.”

✓ located verbatim in the source · abstract, characters 759–946 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Low temperature with sodium benzoate appeared optimal for reducing browning and improving survival.

Treatment
sodium benzoate (0.2 mg/L)e0
Outcome
reducing browning rate and improving survivale0

Not reportedTaxon

Verbatim

e0

“lower temperature plus 20 min 8-HQS (0.002 mg/L) or sodium benzoate (0.2 mg/L) before inoculation appeared to be optimum for reducing browning rate and improving survival of the explants.”

✓ located verbatim in the source · abstract, characters 759–946 · show in text ↑