Abstract
In order to obtain new seedless triploid varieties,hybridation was conducted by three diploid seedless varieties of‘Flame Seedless',‘Sweet Scarlet',and‘Thompson Seedless' used as mother plants and tetraploid variety of‘Kyoho'used as father plant.The effect of sampling dates of immature embryo,culture media,and integument on embryo rescue efficiency was studied.The ploidy of the survival progeny was preliminarily determined by using Flow Cytometer.The results showed that the sampling dates had a significant effect on the seedling survival rate.The suitable dates also varied from the parent varieties,which was 30 d for‘Thompson Seedless',35dfor‘Sweet Scarlet',and 40dfor‘Flame Seedless'.During the embryo germination stage,the embryo germinated better when inoculated on 1/2MS+IAA 0.1mg/L medium.Removing the integument from the embryo led the embryo germinated faster and increased the seedling survival rate significantly.Twenty two progeny seedlingswere preliminarily determined as triploid by Flow Cytometer.
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Claims 8
Claim · model-written summary
Sampling date significantly affected seedling survival.
Not reportedDirection
Verbatim
e0“the sampling dates had a significant effect on the seedling survival rate”
t0“seedless diploid”
Claim · model-written summary
Suitable embryo sampling for Thompson Seedless was 30 d.
Not reportedOutcome
Verbatim
e0“which was 30 d for‘Thompson Seedless',35dfor‘Sweet Scarlet',and 40dfor‘Flame Seedless'”
t0“seedless diploid”
Claim · model-written summary
Suitable embryo sampling for Sweet Scarlet was 35d.
Not reportedOutcome
Verbatim
e0“which was 30 d for‘Thompson Seedless',35dfor‘Sweet Scarlet',and 40dfor‘Flame Seedless'”
t0“seedless diploid”
Claim · model-written summary
Suitable embryo sampling for Flame Seedless was 40d.
Not reportedOutcome
Verbatim
e0“which was 30 d for‘Thompson Seedless',35dfor‘Sweet Scarlet',and 40dfor‘Flame Seedless'”
t0“seedless diploid”
Claim · model-written summary
Embryos germinated better on 1/2MS+IAA 0.1mg/L medium.
- Taxon
- seedless diploidt0
- Treatment
- 1/2MS+IAA 0.1mg/L mediume0
- Outcome
- embryo germinatede0
- Direction
- bettere0
Not reportedComparator
Verbatim
e0“the embryo germinated better when inoculated on 1/2MS+IAA 0.1mg/L medium”
t0“seedless diploid”
Claim · model-written summary
Removing the integument accelerated embryo germination.
- Taxon
- seedless diploidt0
- Treatment
- Removing the integumente0
- Outcome
- embryo germinatede0
- Direction
- fastere0
Not reportedComparator
Verbatim
e0“Removing the integument from the embryo led the embryo germinated faster and increased the seedling survival rate significantly”
t0“seedless diploid”
Claim · model-written summary
Removing the integument significantly increased seedling survival.
- Taxon
- seedless diploidt0
- Treatment
- Removing the integumente0
- Outcome
- seedling survival ratee0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Removing the integument from the embryo led the embryo germinated faster and increased the seedling survival rate significantly”
t0“seedless diploid”
Claim · model-written summary
Twenty two progeny seedlings were preliminarily identified as triploid.
Verbatim
e0“Twenty two progeny seedlingswere preliminarily determined as triploid by Flow Cytometer”
t0“seedless diploid”