Abstract
H 2O 2, a species of active oxygen, at 10 mmol/L could induce stomatal closure and inhibit stomatal opening(Fig.1). The effect of H 2O 2 inducing stomatal closure could be 10 mmol/L V C, but V C in lower concentration(1 mmol/L) had no such effect(Fig.2). EGTA (chelator of Ca 2+ ) and phenothiazine(antagonist of calmodulin) had no effects on the H 2O 2 induced stomatal closure(Fig.3), but they could abolish the effects of 10 μmol/L H 2O 2 on the stomatal aperture in Vicia faba (Fig.4). In addition, the K + channel current in plasma membrane of guard cell was recorded by whole cell configuration of patch clamp. On adding 2 mmol/L H 2O 2, voltage clamp measurements showed that the inward K + channel current significantly decreased 5 min after H 2O 2 treatment, and the outward K + channel current rose markedly about 30 min after H 2O 2 treatment(Fig.6). This suggests that H 2O 2 induced stomatal closure may be the result of the inhibition of K + uptake and the evocation of K + release through K + channels on plasma membrane in guard cells.
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Claims 10
Claim · model-written summary
At 10 mmol/L, peroxide induced stomatal closure.
- Taxon
- Vicia faba
Vicia fabat0 - Treatment
- H 2O 2, a species of active oxygen, at 10 mmol/Le0
- Outcome
- stomatal closuree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H 2O 2, a species of active oxygen, at 10 mmol/L could induce stomatal closure”
t0“Vicia faba Guard Cell”
Claim · model-written summary
At 10 mmol/L, peroxide inhibited stomatal opening.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- H 2O 2, a species of active oxygen, at 10 mmol/Le0
- Outcome
- stomatal openinge0
- Direction
- inhibite0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H 2O 2, a species of active oxygen, at 10 mmol/L could induce stomatal closure and inhibit stomatal opening”
t0“Vicia faba Guard Cell”
Claim · model-written summary
Vitamin C at 10 mmol/L could induce stomatal closure, whereas 1 mmol/L did not.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- 10 mmol/L V Ce0
- Outcome
- stomatal closuree0
- Direction
- inducinge0
- Comparator
- V C in lower concentration(1 mmol/L)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of H 2O 2 inducing stomatal closure could be 10 mmol/L V C, but V C in lower concentration(1 mmol/L) had no such effect”
t0“Vicia faba Guard Cell”
Claim · model-written summary
EGTA did not affect peroxide-induced stomatal closure.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- EGTAe0
- Outcome
- H 2O 2 induced stomatal closuree0
- Direction
- no effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EGTA (chelator of Ca 2+ ) and phenothiazine(antagonist of calmodulin) had no effects on the H 2O 2 induced stomatal closure”
t0“Vicia faba Guard Cell”
Claim · model-written summary
Phenothiazine did not affect peroxide-induced stomatal closure.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- phenothiazinee0
- Outcome
- H 2O 2 induced stomatal closuree0
- Direction
- no effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EGTA (chelator of Ca 2+ ) and phenothiazine(antagonist of calmodulin) had no effects on the H 2O 2 induced stomatal closure”
t0“Vicia faba Guard Cell”
Claim · model-written summary
EGTA abolished the effect of 10 μmol/L peroxide on stomatal aperture.
- Taxon
- Vicia fabaVicia fabae0
- Treatment
- EGTAe0
- Outcome
- effects of 10 μmol/L H 2O 2 on the stomatal aperturee0
- Direction
- abolishe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EGTA (chelator of Ca 2+ ) and phenothiazine(antagonist of calmodulin) had no effects on the H 2O 2 induced stomatal closure(Fig.3), but they could abolish the effects of 10 μmol/L H 2O 2 on the stomatal aperture in Vicia faba”
Claim · model-written summary
Phenothiazine abolished the effect of 10 μmol/L peroxide on stomatal aperture.
- Taxon
- Vicia fabaVicia fabae0
- Treatment
- phenothiazinee0
- Outcome
- effects of 10 μmol/L H 2O 2 on the stomatal aperturee0
- Direction
- abolishe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phenothiazine(antagonist of calmodulin) had no effects on the H 2O 2 induced stomatal closure(Fig.3), but they could abolish the effects of 10 μmol/L H 2O 2 on the stomatal aperture in Vicia faba”
Claim · model-written summary
Peroxide at 2 mmol/L significantly decreased inward guard-cell potassium current after five minutes.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- 2 mmol/L H 2O 2e0
- Outcome
- inward K + channel currente0
- Direction
- decreasede0
- Qualifier
- 5 min after H 2O 2 treatmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On adding 2 mmol/L H 2O 2, voltage clamp measurements showed that the inward K + channel current significantly decreased 5 min after H 2O 2 treatment”
t0“Vicia faba Guard Cell”
Claim · model-written summary
Peroxide at 2 mmol/L markedly raised outward guard-cell potassium current after about 30 minutes.
- Taxon
- Vicia fabaVicia fabat0
- Treatment
- 2 mmol/L H 2O 2e1
- Outcome
- outward K + channel currente0
- Direction
- rosee0
- Qualifier
- about 30 min after H 2O 2 treatmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the outward K + channel current rose markedly about 30 min after H 2O 2 treatment”
e1“2 mmol/L H 2O 2”
t0“Vicia faba Guard Cell”
Claim · model-written summary
The authors suggest that altered potassium uptake and release through guard-cell channels may explain peroxide-induced stomatal closure.
- Taxon
- Vicia fabaVicia fabat0
- Outcome
- H 2O 2 induced stomatal closuree0
- Qualifier
- inhibition of K + uptake and the evocation of K + release through K + channels on plasma membrane in guard cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This suggests that H 2O 2 induced stomatal closure may be the result of the inhibition of K + uptake and the evocation of K + release through K + channels on plasma membrane in guard cells.”
t0“Vicia faba Guard Cell”
The same questions in other papers
hydrogen peroxide · physiology: gas exchange · Vicia faba (faba bean)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.