Abstract
To reveal the molecular mechanism underlying the expression of soybean PLP1( GmPLP1) gene that contains PAS /LOV domain,its 5' regulatory region was analyzed. The1. 5 kb fragment upstream of the GmPLP1 gene was isolated from soybean cultivar‘Dongnong L13'by genome walking. There were 107 cis-acting elements in the promoter predicted by online software Plant CARE and PLACE. the promoter fragment deletions were inserted into the 5' region of the gus. A reporter gene in vector pBI121( replacing the 35S promoter). In order to determine the promoter characteristic of GmPLP1. The constructs were transferred into Nicotiana tabacum and Glycine max. The GUS activity was up-regulated under dark,blue light,gibberellin A3and abscisic acid driven by GmPLP1 promoter.
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Claims 6
Claim · model-written summary
A 1.5 kb upstream fragment of GmPLP1 was isolated from soybean cultivar Dongnong L13.
- Taxon
- soybean
Glycine maxe0 - Material
- Dongnong L13e0
- Gene
- GmPLP1e0
- Outcome
- fragment upstream of the GmPLP1 genee0
- Value
- 1. 5 kbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The1. 5 kb fragment upstream of the GmPLP1 gene was isolated from soybean cultivar‘Dongnong L13'by genome walking.”
Claim · model-written summary
The promoter was predicted to contain 107 cis-acting elements.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- cis-acting elements in the promotere0
- Value
- 107 cis-acting elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were 107 cis-acting elements in the promoter predicted by online software Plant CARE and PLACE.”
t0“Soybean”
Claim · model-written summary
The GmPLP1 promoter increased GUS activity under dark conditions.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GUS activity was up-regulated under dark,blue light,gibberellin A3and abscisic acid driven by GmPLP1 promoter.”
t0“Soybean”
Claim · model-written summary
The GmPLP1 promoter increased GUS activity under blue light.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- blue lighte0
- Gene
- GmPLP1e0
- Outcome
- GUS activitye0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GUS activity was up-regulated under dark,blue light,gibberellin A3and abscisic acid driven by GmPLP1 promoter.”
t0“Soybean”
Claim · model-written summary
The GmPLP1 promoter increased GUS activity under gibberellin A3.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- gibberellin A3e0
- Gene
- GmPLP1e0
- Outcome
- GUS activitye0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GUS activity was up-regulated under dark,blue light,gibberellin A3and abscisic acid driven by GmPLP1 promoter.”
t0“Soybean”
Claim · model-written summary
The GmPLP1 promoter increased GUS activity under abscisic acid.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- abscisic acide0
- Gene
- GmPLP1e0
- Outcome
- GUS activitye0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GUS activity was up-regulated under dark,blue light,gibberellin A3and abscisic acid driven by GmPLP1 promoter.”
t0“Soybean”
The same questions in other papers
light · molecular: expression · Glycine max (soybean)Positive and negative results
11 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- no effect
- no effect
16 papers more in this group
See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.