Abstract
In this report we provide evidence that cytosolic phosphoenolpyruvate carboxylase (PEPC) in soybean (Glycine max L.) root nodules is regulated in vivo by a seryl-phosphorylation cycle, as with the C4, Crassulacean acid metabolism, and C3 leaf isoforms. Pretreatment of parent plants by stem girdling for 5 or 14 h caused a significant decrease in the apparent phosphorylation state of nodule PEPC, as indicated by the 50% inhibition constant (L-malate) and specific activity values assayed at suboptimal conditions, whereas short-term darkness alone was without effect. However, extended (26 h) darkness led to the formation of a relatively dephosphorylated nodule PEPC, an effect that was reversed by illuminating the darkened plants for 3 h. This reversal of the apparent phosphorylation state in the light was prevented by concomitant stem girdling. In contrast, the optimal activity of nodule PEPC and its protein level showed little or no change in all pretreated plants. These results suggest that the phosphorylation state of PEPC in soybean root nodules is possibly modulated by photosynthate transported recently from the shoots. In situ [32P]orthophosphate labeling, immunoprecipitation, and phosphoamino acid analyses confirmed directly that PEPC in detached intact soybean nodules is phosphorylated on a serine residue(s).
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Claims 9
Claim · model-written summary
Stem girdling for 5 or 14 h significantly decreased the apparent phosphorylation state of nodule PEPC.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- nodulee1
- Treatment
- stem girdling for 5 or 14 he1
- Gene
- PEPCe1
- Outcome
- apparent phosphorylation state of nodule PEPCe1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytosolic phosphoenolpyruvate carboxylase (PEPC) in soybean (Glycine max L.) root nodules is regulated in vivo by a seryl-phosphorylation cycle”
e1“Pretreatment of parent plants by stem girdling for 5 or 14 h caused a significant decrease in the apparent phosphorylation state of nodule PEPC”
Claim · model-written summary
Short-term darkness alone had no effect on the apparent phosphorylation state of nodule PEPC.
- Taxon
- soybeanGlycine maxe0
- Treatment
- short-term darkness alonee2
- Gene
- PEPCe1
- Outcome
- apparent phosphorylation state of nodule PEPCe1
- Direction
- no_changeinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytosolic phosphoenolpyruvate carboxylase (PEPC) in soybean (Glycine max L.) root nodules is regulated in vivo by a seryl-phosphorylation cycle”
e1“Pretreatment of parent plants by stem girdling for 5 or 14 h caused a significant decrease in the apparent phosphorylation state of nodule PEPC”
e2“whereas short-term darkness alone was without effect”
Claim · model-written summary
Extended (26 h) darkness led to a relatively dephosphorylated nodule PEPC.
- Taxon
- soybeanGlycine maxe0
- Treatment
- extended (26 h) darknesse3
- Outcome
- nodule PEPCe3
- Direction
- relatively dephosphorylatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytosolic phosphoenolpyruvate carboxylase (PEPC) in soybean (Glycine max L.) root nodules is regulated in vivo by a seryl-phosphorylation cycle”
e3“extended (26 h) darkness led to the formation of a relatively dephosphorylated nodule PEPC, an effect that was reversed by illuminating the darkened plants for 3 h”
Claim · model-written summary
Illuminating the darkened plants for 3 h reversed the dephosphorylation of nodule PEPC caused by extended darkness.
- Taxon
- soybeanGlycine maxe0
- Treatment
- illuminating the darkened plants for 3 he3
- Outcome
- relatively dephosphorylated nodule PEPCe3
- Direction
- reversede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytosolic phosphoenolpyruvate carboxylase (PEPC) in soybean (Glycine max L.) root nodules is regulated in vivo by a seryl-phosphorylation cycle”
e3“extended (26 h) darkness led to the formation of a relatively dephosphorylated nodule PEPC, an effect that was reversed by illuminating the darkened plants for 3 h”
Claim · model-written summary
Stem girdling prevented the reversal of the apparent phosphorylation state in the light.
- Taxon
- soybeanGlycine maxe0
- Treatment
- concomitant stem girdlinge4
- Outcome
- reversal of the apparent phosphorylation state in the lighte4
- Direction
- preventede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytosolic phosphoenolpyruvate carboxylase (PEPC) in soybean (Glycine max L.) root nodules is regulated in vivo by a seryl-phosphorylation cycle”
e4“This reversal of the apparent phosphorylation state in the light was prevented by concomitant stem girdling”
Claim · model-written summary
Pretreated plants showed little or no change in the optimal activity of nodule PEPC and its protein level.
- Taxon
- soybeanGlycine maxe0
- Treatment
- pretreated plantse5
- Outcome
- the optimal activity of nodule PEPC and its protein levele5
- Direction
- little or no changee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytosolic phosphoenolpyruvate carboxylase (PEPC) in soybean (Glycine max L.) root nodules is regulated in vivo by a seryl-phosphorylation cycle”
e5“In contrast, the optimal activity of nodule PEPC and its protein level showed little or no change in all pretreated plants”
Claim · model-written summary
The phosphorylation state of PEPC is possibly modulated by photosynthate transported recently from the shoots.
- Taxon
- soybeanGlycine maxe6
- Gene
- PEPCe6
- Outcome
- phosphorylation state of PEPCe6
- Qualifier
- photosynthate transported recently from the shootse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“These results suggest that the phosphorylation state of PEPC in soybean root nodules is possibly modulated by photosynthate transported recently from the shoots”
Claim · model-written summary
PEPC in detached intact soybean nodules is phosphorylated on a serine residue(s).
- Taxon
- soybeanGlycine maxe7
- Organ / stage
- detached intact soybean nodulese7
- Gene
- PEPCe7
- Outcome
- phosphorylated on a serine residue(s)e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“In situ [32P]orthophosphate labeling, immunoprecipitation, and phosphoamino acid analyses confirmed directly that PEPC in detached intact soybean nodules is phosphorylated on a serine residue(s)”
Claim · model-written summary
Cytosolic PEPC in soybean root nodules is regulated in vivo by a seryl-phosphorylation cycle.
- Taxon
- soybeanGlycine maxe0
- Gene
- cytosolic phosphoenolpyruvate carboxylase (PEPC)e0
- Outcome
- regulated in vivoe0
- Qualifier
- by a seryl-phosphorylation cyclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cytosolic phosphoenolpyruvate carboxylase (PEPC) in soybean (Glycine max L.) root nodules is regulated in vivo by a seryl-phosphorylation cycle”
The same questions in other papers
light · molecular: expression · Glycine max (soybean)Positive and negative results
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- positive
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See all 20 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.