Abstract
The omega3 fatty-acid desaturases: FAD7 and FAD8 (plastid) and FAD3 (reticular) are responsible for trienoic fatty-acid (TA) production in plants. The expression of these enzymes seemed to be regulated differently in response to light. Darkness leads to a decrease in total TA level. Under such conditions, FAD3 and FAD8 transcript levels were undetectable but increased after re-illumination concomitant with TA levels, indicating a transcriptional control. On the contrary, FAD7 transcript levels were similar to illuminated control cells, suggesting the presence of a post-transcriptional control mechanism. Furthermore, FAD7 mRNA stability increased dramatically in darkness. Analysis of FAD7 protein accumulation using specific antibodies revealed that FAD7 was very stable whatever the light or darkness conditions. These results indicate that FAD7 enzyme availability is not limiting for 18:3 production in darkness. Our data point to an additional post-translational regulatory mechanism that controls the activity of FAD7 in response to light.
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Claims 7
Claim · model-written summary
Darkness decreased the total trienoic fatty-acid level in soybean photosynthetic cell suspensions.
- Taxon
- soybean
Glycine maxt0 - Material
- photosynthetic cell suspensionst0
- Treatment
- Darknesse1
- Outcome
- total TA levele1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean photosynthetic cell suspensions”
e1“Darkness leads to a decrease in total TA level”
Claim · model-written summary
FAD3 and FAD8 transcript levels were undetectable in darkness but increased after re-illumination.
- Taxon
- soybeanGlycine maxt0
- Material
- photosynthetic cell suspensionst0
- Treatment
- re-illuminatione2
- Gene
- FAD3 and FAD8e2
- Outcome
- transcript levelse2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean photosynthetic cell suspensions”
e2“FAD3 and FAD8 transcript levels were undetectable but increased after re-illumination”
Claim · model-written summary
FAD7 transcript levels in darkness were similar to those of illuminated control cells.
- Taxon
- soybeanGlycine maxt0
- Material
- photosynthetic cell suspensionst0
- Treatment
- Darknesse1
- Gene
- FAD7e3
- Outcome
- transcript levelse3
- Direction
- no_changeinferrede3
- Comparator
- illuminated control cellse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean photosynthetic cell suspensions”
e1“Darkness leads to a decrease in total TA level”
e3“FAD7 transcript levels were similar to illuminated control cells”
Claim · model-written summary
FAD7 mRNA stability increased dramatically in darkness.
- Taxon
- soybeanGlycine maxt0
- Material
- photosynthetic cell suspensionst0
- Treatment
- darknesse4
- Gene
- FAD7e4
- Outcome
- mRNA stabilitye4
- Direction
- increasede4
- Qualifier
- dramaticallye4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean photosynthetic cell suspensions”
e4“FAD7 mRNA stability increased dramatically in darkness”
Claim · model-written summary
FAD7 protein accumulation was very stable under both light and darkness.
- Taxon
- soybeanGlycine maxt0
- Material
- photosynthetic cell suspensionst0
- Treatment
- light or darkness conditionse5
- Gene
- FAD7e5
- Outcome
- FAD7 protein accumulatione5
- Direction
- no_changeinferrede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean photosynthetic cell suspensions”
e5“Analysis of FAD7 protein accumulation using specific antibodies revealed that FAD7 was very stable whatever the light or darkness conditions”
Claim · model-written summary
FAD7 enzyme availability is not limiting for 18:3 production in darkness.
- Taxon
- soybeanGlycine maxt0
- Treatment
- darknesse6
- Gene
- FAD7e6
- Outcome
- 18:3 productione6
- Qualifier
- enzyme availability is not limitinge6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean photosynthetic cell suspensions”
e6“FAD7 enzyme availability is not limiting for 18:3 production in darkness”
Claim · model-written summary
An additional post-translational regulatory mechanism controls FAD7 activity in response to light.
- Taxon
- soybeanGlycine maxt0
- Treatment
- lighte7
- Gene
- FAD7e7
- Outcome
- activity of FAD7e7
- Qualifier
- post-translational regulatory mechanisme7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“soybean photosynthetic cell suspensions”
e7“Our data point to an additional post-translational regulatory mechanism that controls the activity of FAD7 in response to light”
The same questions in other papers
light · molecular: expression · Glycine max (soybean)Positive and negative results
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- positive
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