Abstract
A full-length cDNA encoding PAL was cloned from leaves of plantain (Musa ABB cv. Dongguandajiao) by RT-PCR and RACE. The cDNA was 1 300 bp in length and contained a complete ORF of 1 191 bp which encodes a protein of 397 amino acid residues. Its deduced amino acid sequence had 89% identity with rice PAL and then this gene was hence designated as M-PAL. Southern hybridization analysis demonstrated that a small family of four to five PAL genes exists in plantain banana. The complete coding sequence of M-PAL was cloned into an expression vector pET32 (a+) and a protein about 43 KD expressed in Escherichia coli BL21 (DE3) was the same as deduced molecular weight and showed phenylalanine ammonia-lyase activity. The transcript of M-PAL gene in the leaves was increased when the plantlets of plantain were inoculated with Fusarium oxysporum f. sp. cubense, indicating that the expression of M-PAL may be related to resistance of banana Fusarium wilt.
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Claims 9
Claim · model-written summary
A full-length PAL cDNA was cloned from plantain leaves.
- Taxon
- plantainMusa ABB Groupe0
- Gene
- PALe0
- Outcome
- full-length cDNA encoding PALe0
- Value
- 1 300 bpe1
Verbatim
e0“A full-length cDNA encoding PAL was cloned from leaves of plantain (Musa ABB cv. Dongguandajiao)”
e1“The cDNA was 1 300 bp in length”
Claim · model-written summary
The PAL cDNA contained a 1 191 bp complete ORF.
- Taxon
- plantainMusa ABB Groupe0
- Gene
- ORFe1
- Outcome
- complete ORFe1
- Value
- 1 191 bpe1
Verbatim
e0“plantain (Musa ABB cv. Dongguandajiao)”
e1“contained a complete ORF of 1 191 bp”
Claim · model-written summary
The PAL ORF encoded a 397 amino-acid protein.
- Taxon
- plantainMusa ABB Groupe0
- Outcome
- proteine1
- Value
- 397 amino acid residuese1
Verbatim
e0“plantain (Musa ABB cv. Dongguandajiao)”
e1“encodes a protein of 397 amino acid residues”
Claim · model-written summary
The deduced PAL sequence showed 89% identity with rice PAL.
- Taxon
- plantainMusa ABB Groupe0
- Gene
- PALe1
- Outcome
- identity with rice PALe1
- Value
- 89%e1
Verbatim
e0“plantain (Musa ABB cv. Dongguandajiao)”
e1“deduced amino acid sequence had 89% identity with rice PAL”
Claim · model-written summary
Plantain has a small family of four to five PAL genes.
Verbatim
e0“a small family of four to five PAL genes exists in plantain banana”
Claim · model-written summary
Recombinant plantain PAL protein was about 43 KD and matched its predicted molecular weight.
- Taxon
- plantainMusa ABB Groupe0
- Gene
- proteine1
- Outcome
- deduced molecular weighte1
- Value
- 43 KDe1
Verbatim
e0“plantain (Musa ABB cv. Dongguandajiao)”
e1“a protein about 43 KD expressed in Escherichia coli BL21 (DE3) was the same as deduced molecular weight”
Claim · model-written summary
The recombinant plantain protein showed phenylalanine ammonia-lyase activity.
- Taxon
- plantainMusa ABB Groupe0
- Outcome
- phenylalanine ammonia-lyase activitye1
Verbatim
e0“plantain (Musa ABB cv. Dongguandajiao)”
e1“showed phenylalanine ammonia-lyase activity”
Claim · model-written summary
M-PAL transcript increased after plantain inoculation with Fusarium oxysporum f. sp. cubense.
- Taxon
- plantainMusa ABB Groupe0
- Organ / stage
- leavese1
- Treatment
- inoculated with Fusarium oxysporum f. sp. cubensee0
- Interactor
- Fusarium oxysporum f. sp. cubense
Fusarium oxysporum f. sp. cubensee0pathogen - Gene
- M-PALe1
- Outcome
- transcript of M-PAL genee1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plantlets of plantain were inoculated with Fusarium oxysporum f. sp. cubense”
e1“The transcript of M-PAL gene in the leaves was increased when the plantlets of plantain were inoculated with Fusarium oxysporum f. sp. cubense”
Claim · model-written summary
The authors suggest M-PAL expression may relate to Fusarium wilt resistance.
- Taxon
- plantainMusa ABB Groupe0
- Interactor
- Fusarium oxysporum f. sp. cubenseFusarium oxysporum f. sp. cubensee2pathogen
- Gene
- M-PALe1
- Outcome
- resistance of banana Fusarium wilte1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plantain (Musa ABB cv. Dongguandajiao)”
e1“expression of M-PAL may be related to resistance of banana Fusarium wilt”
e2“Fusarium oxysporum f. sp. cubense”