Abstract
The changes in content and form of polyamines(PAs) in the leaves of two soybean (Glycine max L.) cultivar seedlings differing in drought tolerance were investigated with HPLC under osmotic stress.Yudou No.24 cv.(drought-tolerant) showed higher increases magnitude in free-spermidine(fSpd) and free-spermine(fSpm) in leaves than Yudou No.6 cv.(drought-sensitive) after treated with polyethylene glycol(PEG) 6,000 for 7 d,while Yudou No.6 cv.showed a higher increased magnitude in free putrescine(fPut).Thus Yudou No.24 cv.gave a higher ratio of(fSpd + fSpm) / fPut than Yudou No.6 cv.in leaves under osmotic stress.Exogenous treatment of seedlings with methylglyoxal-bis(guanylhydrazone)(MGBG),an S-adenosylmethionine decarboxylase(SAMDC,which catalyzes the Spd and Sam biosynthesis) inhibitor,resulted in a reduction of fSpd and fSpm in leaves and aggravation of PEG-induced injury to Yudou No.24 cv.seedlings,while exogenous Spd treatment resulted in an increase in fSpd + fSpm in leaves,furthermore retarded PEG-induced injury in Yudou No.6 cv.seedling.There was no statistically significant difference between the contents of acid soluble covalently conjugated PAs(ASCC-PAs) in leaves of both cultivars under PEG osmotic stress,suggesting that the level of ASCC-PAs in leaves had no relationship with the osmotic stress tolerance of wheat seedlings.Osmotic stress induced a significant increase in acid insoluble covalently conjugated polyamines(AISCC-PAs) level in seedling leaves of Yudou No.24 cv.,whereas it had a slightly influence on AISCC-PAs in Yudou No.6 cv.seedlings.Exogenous treatment of seedlings with phenanthrolin(o-Phen),an inhibitor of transglutaminase(TGase,which catalyzes AISCC-PAs biosynthesis),also aggravated PEG-induced injury to Yudou No.24 cv.seedlings,combined with a decrease in AISCC-PAs levels.The results suggested that fSpd,fSpm and PIS-Pas,which strengthened the osmotic stress tolerant capacity of soybean seedlings.
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Claims 13
Claim · model-written summary
Yudou No.24 had a greater rise in free spermidine than Yudou No.6 after PEG treatment.
- Material
- Yudou No.24 cv.e0
- Treatment
- polyethylene glycol(PEG) 6,000 for 7 de0
- Outcome
- free-spermidine(fSpd)e0
- Direction
- higher increasese0
- Comparator
- Yudou No.6 cv.e0
Not reportedTaxon
Verbatim
e0“Yudou No.24 cv.(drought-tolerant) showed higher increases magnitude in free-spermidine(fSpd) and free-spermine(fSpm) in leaves than Yudou No.6 cv.(drought-sensitive) after treated with polyethylene glycol(PEG) 6,000 for 7 d”
Claim · model-written summary
Yudou No.24 had a greater rise in free spermine than Yudou No.6 after PEG treatment.
- Material
- Yudou No.24 cv.e0
- Treatment
- polyethylene glycol(PEG) 6,000 for 7 de0
- Outcome
- free-spermine(fSpm)e0
- Direction
- higher increasese0
- Comparator
- Yudou No.6 cv.e0
Not reportedTaxon
Verbatim
e0“Yudou No.24 cv.(drought-tolerant) showed higher increases magnitude in free-spermidine(fSpd) and free-spermine(fSpm) in leaves than Yudou No.6 cv.(drought-sensitive) after treated with polyethylene glycol(PEG) 6,000 for 7 d”
Claim · model-written summary
Yudou No.6 had the greater rise in free putrescine.
Not reportedTaxon
Verbatim
e0“Yudou No.6 cv.showed a higher increased magnitude in free putrescine(fPut)”
Claim · model-written summary
Yudou No.24 had a higher leaf (fSpd + fSpm) / fPut ratio under osmotic stress than Yudou No.6.
- Material
- Yudou No.24 cv.e0
- Outcome
- ratio of(fSpd + fSpm) / fPute0
- Direction
- highere0
- Comparator
- Yudou No.6 cv.e0
Not reportedTaxon
Verbatim
e0“Yudou No.24 cv.gave a higher ratio of(fSpd + fSpm) / fPut than Yudou No.6 cv.in leaves under osmotic stress”
Claim · model-written summary
MGBG reduced fSpd and fSpm in Yudou No.24 leaves.
- Treatment
- methylglyoxal-bis(guanylhydrazone)(MGBG)e0
- Outcome
- fSpd and fSpm in leavese0
- Direction
- reductione0
Not reportedTaxonComparator
Verbatim
e0“Exogenous treatment of seedlings with methylglyoxal-bis(guanylhydrazone)(MGBG),an S-adenosylmethionine decarboxylase(SAMDC,which catalyzes the Spd and Sam biosynthesis) inhibitor,resulted in a reduction of fSpd and fSpm in leaves and aggravation of PEG-induced injury to Yudou No.24 cv.seedlings”
Claim · model-written summary
MGBG aggravated PEG injury in Yudou No.24 seedlings.
- Treatment
- methylglyoxal-bis(guanylhydrazone)(MGBG)e0
- Outcome
- PEG-induced injurye0
- Direction
- aggravatione0
Not reportedTaxonComparator
Verbatim
e0“Exogenous treatment of seedlings with methylglyoxal-bis(guanylhydrazone)(MGBG),an S-adenosylmethionine decarboxylase(SAMDC,which catalyzes the Spd and Sam biosynthesis) inhibitor,resulted in a reduction of fSpd and fSpm in leaves and aggravation of PEG-induced injury to Yudou No.24 cv.seedlings”
Claim · model-written summary
Exogenous Spd increased leaf fSpd + fSpm in Yudou No.6.
Not reportedTaxonComparator
Verbatim
e0“exogenous Spd treatment resulted in an increase in fSpd + fSpm in leaves,furthermore retarded PEG-induced injury in Yudou No.6 cv.seedling”
Claim · model-written summary
Exogenous Spd retarded PEG injury in Yudou No.6.
Not reportedTaxonComparator
Verbatim
e0“exogenous Spd treatment resulted in an increase in fSpd + fSpm in leaves,furthermore retarded PEG-induced injury in Yudou No.6 cv.seedling”
Claim · model-written summary
Leaf ASCC-PAs did not differ significantly between the two cultivars under PEG stress.
- Outcome
- contents of acid soluble covalently conjugated PAs(ASCC-PAs)e0
- Direction
- no statistically significant differencee0
Not reportedTaxon
Verbatim
e0“There was no statistically significant difference between the contents of acid soluble covalently conjugated PAs(ASCC-PAs) in leaves of both cultivars under PEG osmotic stress”
Claim · model-written summary
Osmotic stress significantly increased AISCC-PAs in Yudou No.24 leaves.
- Treatment
- Osmotic stresse0
- Outcome
- acid insoluble covalently conjugated polyamines(AISCC-PAs) levele0
- Direction
- increasee0
Not reportedTaxonComparator
Verbatim
e0“Osmotic stress induced a significant increase in acid insoluble covalently conjugated polyamines(AISCC-PAs) level in seedling leaves of Yudou No.24 cv.”
Claim · model-written summary
Osmotic stress had a slight effect on AISCC-PAs in Yudou No.6.
- Outcome
- AISCC-PAse0
Not reportedTaxonTreatmentComparatorDirection
Verbatim
e0“it had a slightly influence on AISCC-PAs in Yudou No.6 cv.seedlings”
Claim · model-written summary
Phenanthrolin aggravated PEG injury in Yudou No.24.
Not reportedTaxonComparator
Verbatim
e0“Exogenous treatment of seedlings with phenanthrolin(o-Phen),an inhibitor of transglutaminase(TGase,which catalyzes AISCC-PAs biosynthesis),also aggravated PEG-induced injury to Yudou No.24 cv.seedlings,combined with a decrease in AISCC-PAs levels”
Claim · model-written summary
Phenanthrolin decreased AISCC-PAs in Yudou No.24.
Not reportedTaxonComparator
Verbatim
e0“Exogenous treatment of seedlings with phenanthrolin(o-Phen),an inhibitor of transglutaminase(TGase,which catalyzes AISCC-PAs biosynthesis),also aggravated PEG-induced injury to Yudou No.24 cv.seedlings,combined with a decrease in AISCC-PAs levels”