Paper · 1997 · Acta Biologiae Experimentalis Sinica

CULTIVATION OF MICROSPORE MECHANICALLY ISOLATED FROM CEREAL FLORETS

Hui Wu

6
claims
7
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

A system of cultivation of micros-pores mechanically isolated from cereal spikes/florets (Segments of barley spikes, florets of rice and wheat) has been successfully established. A large number of green regenerats have been achieved repeatedly in two barley varieties, green plant regeneration was recovered in a wide compatible rice variety 02428. In three wheat varieties, microspore derived mul-ticellular structures (MCS) and early embryo-like structures (ELS) have been obtained. Long duration of cold pretreat-ment of spikes was beneficial to the production of ELS in barley and MCS in wheat. The efficiency of the procedure of microspore isolation, the effects of diploid tissue, air exchange condition and the advantage of early transfer of microspore derived calli to regeneration medium have been proven to improve the regeneration of green plants.

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Claims 6

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

The culture system yielded many green regenerants in two barley varieties.

Material
two barley varietiese0
Outcome
green regeneratse0
Value
A large numbere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A large number of green regenerats have been achieved repeatedly in two barley varieties”

✓ located verbatim in the source · abstract, characters 179–267 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Green plant regeneration was recovered in rice variety 02428.

Material
02428e0
Outcome
green plant regeneratione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

e0

“green plant regeneration was recovered in a wide compatible rice variety 02428”

✓ located verbatim in the source · abstract, characters 269–347 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Wheat microspores formed multicellular structures and early embryo-like structures.

Material
three wheat varietiese0
Outcome
microspore derived mul-ticellular structures (MCS) and early embryo-like structures (ELS)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“In three wheat varieties, microspore derived mul-ticellular structures (MCS) and early embryo-like structures (ELS) have been obtained”

✓ located verbatim in the source · abstract, characters 349–483 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Long cold pretreatment benefited early embryo-like structure production in barley.

Treatment
Long duration of cold pretreat-ment of spikese0
Outcome
production of ELSe0
Direction
beneficiale0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Long duration of cold pretreat-ment of spikes was beneficial to the production of ELS in barley”

✓ located verbatim in the source · abstract, characters 485–580 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Long cold pretreatment benefited multicellular structure production in wheat.

Treatment
Long duration of cold pretreat-ment of spikese0
Outcome
MCS in wheate0
Direction
beneficiale0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Long duration of cold pretreat-ment of spikes was beneficial to the production of ELS in barley and MCS in wheat”

✓ located verbatim in the source · abstract, characters 485–597 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Microspore isolation procedure and diploid tissue improved green plant regeneration.

Taxon
CEREALt0
Outcome
regeneration of green plantse0
Direction
improvee0

Not reportedValue

Verbatim

e0

“The efficiency of the procedure of microspore isolation, the effects of diploid tissue, air exchange condition and the advantage of early transfer of microspore derived calli to regeneration medium have been proven to improve the regeneration of green plants”

✓ located verbatim in the source · abstract, characters 599–857 · show in text ↑
t0

“CEREAL”

✓ located verbatim in the source · title, characters 53–59 · show in text ↑

cold · organism · Triticum aestivum (wheat)Positive and negative results

7 papers: 5 positive, 2 negative or no effect

4 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. no effect
  3. positive

3 papers more in this group

See all 7 papers →

cold · yield · Hordeum vulgare (barley)Positive and negative results

8 papers: 3 positive, 5 negative or no effect

2 papers in the same direction as this paper · 5 in the opposite direction

  1. negative
  2. negative
  3. negative

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.