Abstract
The Agrobacterium-mediated transient expression analysis in intact tissues has emerged as a rapid and useful method to study gene silencing in plants.Potato virus X vector induced silencing of the phytoene desaturase gene(PDS) by Agrobacterium in Nicotiana benthamiana is reported in this paper.The vector construct carried a 409-nucleotide cDNA fragment of the PDS gene.The results showed that the expression of PDS endogenous gene could be inactivated by the expression of 409-bp homologous cDNA fragment carried in the virus vector.
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Claims 1
Claim · model-written summary
A virus-vector 409-bp homologous PDS cDNA fragment inactivated endogenous PDS expression in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- expression of 409-bp homologous cDNA fragment carried in the virus vectore1
- Interactor
- Potato virus XPotato virus Xe0pathogen
- Second interactor
- Agrobacterium
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Agrobacteriume0other - Gene
- PDSe1
- Outcome
- expression of PDS endogenous genee1
- Direction
- inactivatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“Potato virus X vector induced silencing of the phytoene desaturase gene(PDS) by Agrobacterium in Nicotiana benthamiana”
e1“The results showed that the expression of PDS endogenous gene could be inactivated by the expression of 409-bp homologous cDNA fragment carried in the virus vector”
The same questions in other papers
transgenic · Potato virus X · Nicotiana benthamianaPositive and negative results
1 paper in the same direction as this paper · 12 in the opposite direction
- negative
- negative
- negative
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.