Abstract
RNA silencing is a mechanism which higher plants and animals have evolved to defend against viral infection in addition to regulation of gene expression for growth and development. As a counterdefense, many plant and some animal viruses studied to date encode RNA silencing suppressors (RSS) that interfere with various steps of the silencing pathway. In this study, we report the first identification of an RSS from a plant double-stranded RNA (dsRNA) virus. Pns10, encoded by S10 of Rice dwarf phytoreovirus (RDV), exhibited RSS activity in coinfiltration assays with the reporter green fluorescent protein (GFP) in transgenic Nicotiana benthamiana line 16c carrying GFP. The other gene segments of the RDV genome did not have such a function. Pns10 suppressed local and systemic silencing induced by sense RNA but did not interfere with local and systemic silencing induced by dsRNA. Expression of Pns10 also increased the expression of beta-glucuronidase in transient assays and enhanced Potato virus X pathogenicity in N. benthamiana. Collectively, our results establish Pns10 as an RSS encoded by a plant dsRNA virus and further suggest that Pns10 targets an upstream step of dsRNA formation in the RNA silencing pathway.
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Claims 7
Claim · model-written summary
RDV Pns10 showed RNA silencing suppressor activity in GFP reporter assays in transgenic Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Material
- line 16ce0
- Interactor
- Rice dwarf phytoreovirusRice dwarf viruse0pathogen
- Gene
- Pns10e0
- Outcome
- RSS activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pns10, encoded by S10 of Rice dwarf phytoreovirus (RDV), exhibited RSS activity in coinfiltration assays with the reporter green fluorescent protein (GFP) in transgenic Nicotiana benthamiana line 16c carrying GFP”
Claim · model-written summary
Other RDV genome segments lacked RNA silencing suppressor activity.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- RDVe0pathogen
- Outcome
- did not have such a functione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The other gene segments of the RDV genome did not have such a function”
t0“Plant Double-Stranded RNA Virus”
e1“transgenic Nicotiana benthamiana line 16c carrying GFP”
Claim · model-written summary
Pns10 suppressed local and systemic silencing triggered by sense RNA.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Pns10e0
- Gene
- Pns10e0
- Outcome
- local and systemic silencing induced by sense RNAe0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pns10 suppressed local and systemic silencing induced by sense RNA but did not interfere with local and systemic silencing induced by dsRNA”
e1“in transgenic Nicotiana benthamiana line 16c carrying GFP”
Claim · model-written summary
Pns10 did not interfere with local or systemic silencing triggered by dsRNA.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Pns10e0
- Gene
- Pns10e0
- Outcome
- local and systemic silencing induced by dsRNAe0
- Direction
- did not interferee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pns10 suppressed local and systemic silencing induced by sense RNA but did not interfere with local and systemic silencing induced by dsRNA”
e1“in transgenic Nicotiana benthamiana line 16c carrying GFP”
Claim · model-written summary
Pns10 expression increased beta-glucuronidase expression in transient assays.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Expression of Pns10e0
- Gene
- Pns10e0
- Outcome
- expression of beta-glucuronidasee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of Pns10 also increased the expression of beta-glucuronidase in transient assays”
e1“in transgenic Nicotiana benthamiana line 16c carrying GFP”
Claim · model-written summary
Pns10 expression enhanced Potato virus X pathogenicity in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Expression of Pns10e0
- Interactor
- Potato virus XPotato virus Xe0pathogen
- Gene
- Pns10e0
- Outcome
- Potato virus X pathogenicitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of Pns10 also increased the expression of beta-glucuronidase in transient assays and enhanced Potato virus X pathogenicity in N. benthamiana”
Claim · model-written summary
The authors suggest Pns10 acts upstream of dsRNA formation in the silencing pathway.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Gene
- Pns10e0
- Outcome
- RNA silencing pathwaye0
- Qualifier
- an upstream step of dsRNA formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pns10 targets an upstream step of dsRNA formation in the RNA silencing pathway”
e1“N. benthamiana”
The same questions in other papers
transgenic · Potato virus X · Nicotiana benthamianaPositive and negative results
11 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- negative
10 papers more in this group
See all 14 papers →transgenic · molecular: expression · Nicotiana benthamianaPositive and negative results
19 papers in the same direction as this paper · 9 in the opposite direction
- negative
- negative
- no effect
25 papers more in this group
See all 29 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.