Claim · model-written summary
Expression of TMV ORF6 failed to suppress gene silencing in Nicotiana benthamiana.
- Taxon
- N. benthamiana
Nicotiana benthamianae6 - Treatment
- expression of TMV ORF6e6
- Outcome
- gene silencinge6
- Direction
- failed to suppresse6
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“In three different types of assay carried out in N. benthamiana plants, expression of TMV ORF6 failed to suppress gene silencing”
Title
ORF6 of Tobacco mosaic virus is a determinant of viral pathogenicity in Nicotiana benthamiana
Tomas Canto; Stuart A. MacFarlane; Peter Palukaitis Journal of General Virology 2004. doi:10.1099/vir.0.80270-0
Other claims from this paper (10)
- Intervention effect Eliminating ORF6 from TMV attenuated host responses in Nicotiana benthamiana.
- Intervention effect Eliminating ORF6 from TMV did not change virus accumulation in Nicotiana benthamiana.
- Intervention effect Heterologous expression of TMV ORF6 from PVX enhanced PVX virulence in Nicotiana benthamiana.
- Intervention effect Heterologous expression of TMV ORF6 from TRV enhanced TRV virulence in Nicotiana benthamiana.
- Intervention effect Heterologous expression of TMV ORF6 did not change accumulation of PVX or TRV in Nicotiana benthamiana.
- Intervention effect The presence or absence of TMV ORF6 had no effect on host response in N. tabacum infected with TMV or PVX.
- Intervention effect TMV ORF6 had no effect on the synergism between TMV and PVX in N. tabacum.
- Intervention effect The presence of TMV ORF6 had an effect on the pathogenicity of a TRV vector in N. tabacum.
- Observation The encoded 4.8 kDa ORF6 protein fused to GFP showed plasmodesmatal targeting in N. benthamiana epidermal cells.
- Observation The encoded 4.8 kDa ORF6 protein fused to GFP showed widespread cytosolic fluorescence in N. benthamiana epidermal cells.