Abstract
DNA binding with one finger (Dof) proteins constitute a ubiquitous plant-specific transcription factor (TF) family associated with diverse biological processes, including ripening. We conducted a genome-wide analysis of durian (Durio zibethinus Murr.) and identified 24 durian Dofs (DzDofs), 15 of which were expressed in fruit pulp. Gene expression analysis revealed differential expression of DzDofs during ripening in two commercial durian cultivars from Thailand, Monthong and Chanee. Comparing the expression levels of fruit pulp-expressed DzDofs between cultivars revealed ten potential cultivar-dependent Dofs, among which DzDof2.2 showed a significantly greater fold increase at every ripening stage in Chanee than in Monthong. The prediction of DzDof2.2's function based on its orthologue in Arabidopsis revealed its possible role in regulating auxin biosynthesis. We observed significantly higher auxin levels during ripening of Chanee than Monthong which concurred with the greater expression of auxin biosynthetic genes. Transient expression of DzDof2.2 in Nicotiana benthamiana significantly upregulated the expression levels of auxin biosynthetic genes. Higher expression levels of DzDof2.2 in Chanee would enhance auxin levels through transcriptional regulation of auxin biosynthetic genes. Higher auxin levels in Chanee could activate auxin-mediated transcription, contributing to its faster ripening compared to Monthong through earlier initiation of the ethylene response (auxin-ethylene crosstalk).
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Claims 11
Claim · model-written summary
The genome analysis identified 24 durian Dof genes.
- Taxon
- durian
Durio zibethinust0 - Gene
- durian Dofse0
- Outcome
- durian Dofse0
- Value
- 24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified 24 durian Dofs (DzDofs), 15 of which were expressed in fruit pulp.”
t0“durian”
Claim · model-written summary
Fifteen DzDofs were expressed in fruit pulp.
- Taxon
- durianDurio zibethinust0
- Organ / stage
- fruit pulpe0
- Gene
- DzDofse0
- Outcome
- expressed in fruit pulpe0
- Value
- 15e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified 24 durian Dofs (DzDofs), 15 of which were expressed in fruit pulp.”
t0“durian”
Claim · model-written summary
DzDofs showed differential expression during ripening in Monthong and Chanee.
- Taxon
- durianDurio zibethinust0
- Material
- Monthong and Chaneee0
- Organ / stage
- ripeninge0
- Gene
- DzDofse0
- Outcome
- differential expressione0
- Setting
- Thailande0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene expression analysis revealed differential expression of DzDofs during ripening in two commercial durian cultivars from Thailand, Monthong and Chanee.”
t0“durian”
Claim · model-written summary
Ten fruit pulp Dofs were potentially cultivar dependent.
- Taxon
- durianDurio zibethinust0
- Organ / stage
- fruit pulpe0
- Gene
- DzDofse0
- Outcome
- cultivar-dependent Dofse0
- Value
- tene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparing the expression levels of fruit pulp-expressed DzDofs between cultivars revealed ten potential cultivar-dependent Dofs”
t0“durian”
Claim · model-written summary
DzDof2.2 increased more at each ripening stage in Chanee than Monthong.
- Taxon
- durianDurio zibethinust0
- Material
- Chaneee0
- Organ / stage
- ripening stagee0
- Gene
- DzDof2.2e0
- Outcome
- fold increasee0
- Direction
- greatere0
- Comparator
- Monthonge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DzDof2.2 showed a significantly greater fold increase at every ripening stage in Chanee than in Monthong.”
t0“durian”
Claim · model-written summary
Orthologue prediction suggested a possible DzDof2.2 role in auxin biosynthesis.
- Taxon
- durianDurio zibethinust0
- Gene
- DzDof2.2e0
- Outcome
- regulating auxin biosynthesise0
- Qualifier
- possible rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The prediction of DzDof2.2's function based on its orthologue in Arabidopsis revealed its possible role in regulating auxin biosynthesis.”
t0“durian”
Claim · model-written summary
Auxin levels were higher during Chanee ripening than Monthong.
- Taxon
- durianDurio zibethinust0
- Material
- Chaneee0
- Organ / stage
- ripeninge0
- Outcome
- auxin levelse0
- Direction
- highere0
- Comparator
- Monthonge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We observed significantly higher auxin levels during ripening of Chanee than Monthong which concurred with the greater expression of auxin biosynthetic genes.”
t0“durian”
Claim · model-written summary
Auxin biosynthetic genes had greater expression alongside higher auxin in Chanee.
- Taxon
- durianDurio zibethinust0
- Organ / stage
- ripeninge0
- Gene
- auxin biosynthetic genese0
- Outcome
- expression of auxin biosynthetic genese0
- Direction
- greatere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We observed significantly higher auxin levels during ripening of Chanee than Monthong which concurred with the greater expression of auxin biosynthetic genes.”
t0“durian”
Claim · model-written summary
Transient DzDof2.2 expression in Nicotiana benthamiana increased auxin biosynthetic gene expression.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Transient expression of DzDof2.2e0
- Gene
- DzDof2.2e0
- Outcome
- expression levels of auxin biosynthetic genese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of DzDof2.2 in Nicotiana benthamiana significantly upregulated the expression levels of auxin biosynthetic genes.”
t0“durian”
Claim · model-written summary
The authors infer that DzDof2.2 promotes auxin through transcriptional regulation in Chanee.
- Taxon
- durianDurio zibethinust0
- Material
- Chaneee0
- Gene
- DzDof2.2e0
- Outcome
- auxin levelse0
- Qualifier
- transcriptional regulation of auxin biosynthetic genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher expression levels of DzDof2.2 in Chanee would enhance auxin levels through transcriptional regulation of auxin biosynthetic genes.”
t0“durian”
Claim · model-written summary
The authors propose higher auxin contributes to faster Chanee ripening through earlier ethylene response.
- Taxon
- durianDurio zibethinust0
- Material
- Chaneee0
- Compound
- auxine0
- Outcome
- faster ripeninge0
- Qualifier
- earlier initiation of the ethylene responsee0
- Comparator
- Monthonge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Higher auxin levels in Chanee could activate auxin-mediated transcription, contributing to its faster ripening compared to Monthong through earlier initiation of the ethylene response (auxin-ethylene crosstalk).”
t0“durian”
The same questions in other papers
transgenic · molecular: expression · Nicotiana benthamianaPositive and negative results
19 papers in the same direction as this paper · 9 in the opposite direction
- positive
- negative
- negative
25 papers more in this group
See all 29 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.