Abstract
Root endodermis, the innermost cortical layer surrounding the root vasculature, serves as the foremost barrier to water, solutes, and nutrients taken up from soil. Endodermis barrier functionality is achieved via its hydrophobic coating of lignified Casparian strips and the suberin lamellae; nonetheless the regulatory mechanisms underlying endodermis suberization are still elusive. Here, we discovered that the Arabidopsis SUBERMAN (SUB) transcription factor controls the establishment of the root suberin lamellae. Transient expression of SUB in Nicotiana benthamiana leaves resulted in the induction of heterologous suberin genes, the accumulation of suberin-type monomers, and consequent deposition of suberin-like lamellae. We demonstrate that SUB exerts its regulatory roles by transactivating promoters of suberin genes. In Arabidopsis, SUB is expressed in patchy and continuous suberization root endodermal cells, and thus roots with higher or lower expression of SUB display altered suberin polymer deposition patterns and modified composition. While these changes did not interfere with Casparian strip formation they had a substantial effect on root uptake capacity, resulting in varied root and leaf ionomic phenotypes. Gene expression profiling revealed that SUB function impacts transcriptional networks associated with suberin, phenylpropanoids, lignin, and cuticular lipid biosynthesis, as well as root transport activities, hormone signalling, and cell wall modification. Our findings highlight SUB as a regulator of root endodermis suberization during normal development, and its characterization is thus a key step towards dissecting the molecular mechanisms partaking in root endodermal barrier functionalities.
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Claims 13
Claim · model-written summary
SUB controls the establishment of the root suberin lamellae in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise1 - Organ / stage
- roote1
- Gene
- SUBERMAN (SUB) transcription factore1
- Outcome
- establishment of the root suberin lamellaee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Arabidopsis SUBERMAN (SUB) transcription factor controls the establishment of the root suberin lamellae”
Claim · model-written summary
Transient expression of SUB in Nicotiana benthamiana leaves induced heterologous suberin genes.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae2 - Organ / stage
- leavese2
- Treatment
- Transient expression of SUBe2
- Gene
- SUBe2
- Outcome
- heterologous suberin genese2
- Direction
- inductione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Transient expression of SUB in Nicotiana benthamiana leaves resulted in the induction of heterologous suberin genes, the accumulation of suberin-type monomers, and consequent deposition of suberin-like lamellae”
Claim · model-written summary
Transient expression of SUB in Nicotiana benthamiana leaves led to accumulation of suberin-type monomers.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae2
- Organ / stage
- leavese2
- Treatment
- Transient expression of SUBe2
- Gene
- SUBe2
- Outcome
- suberin-type monomerse2
- Direction
- accumulatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Transient expression of SUB in Nicotiana benthamiana leaves resulted in the induction of heterologous suberin genes, the accumulation of suberin-type monomers, and consequent deposition of suberin-like lamellae”
Claim · model-written summary
Transient expression of SUB in Nicotiana benthamiana leaves led to deposition of suberin-like lamellae.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae2
- Organ / stage
- leavese2
- Treatment
- Transient expression of SUBe2
- Gene
- SUBe2
- Outcome
- deposition of suberin-like lamellaee2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Transient expression of SUB in Nicotiana benthamiana leaves resulted in the induction of heterologous suberin genes, the accumulation of suberin-type monomers, and consequent deposition of suberin-like lamellae”
Claim · model-written summary
SUB exerts its regulatory role in Arabidopsis by transactivating promoters of suberin genes.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SUBe3
- Outcome
- transactivating promoters of suberin genese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Arabidopsis SUBERMAN (SUB) transcription factor controls the establishment of the root suberin lamellae”
e3“We demonstrate that SUB exerts its regulatory roles by transactivating promoters of suberin genes”
Claim · model-written summary
In Arabidopsis, SUB is expressed in patchy and continuous suberizing root endodermal cells.
- Taxon
- ArabidopsisArabidopsise4
- Organ / stage
- root endodermal cellse4
- Gene
- SUBe4
- Outcome
- expressione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“In Arabidopsis, SUB is expressed in patchy and continuous suberization root endodermal cells, and thus roots with higher or lower expression of SUB display altered suberin polymer deposition patterns and modified composition”
Claim · model-written summary
Roots with higher or lower expression of SUB display altered suberin polymer deposition patterns and modified composition.
- Taxon
- ArabidopsisArabidopsise4
- Organ / stage
- rootse4
- Treatment
- higher or lower expression of SUBe4
- Outcome
- suberin polymer deposition patterns and modified compositione4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“In Arabidopsis, SUB is expressed in patchy and continuous suberization root endodermal cells, and thus roots with higher or lower expression of SUB display altered suberin polymer deposition patterns and modified composition”
Claim · model-written summary
Altered SUB expression did not interfere with Casparian strip formation in Arabidopsis roots.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- higher or lower expression of SUBe4
- Outcome
- Casparian strip formatione5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Arabidopsis SUBERMAN (SUB) transcription factor controls the establishment of the root suberin lamellae”
e4“In Arabidopsis, SUB is expressed in patchy and continuous suberization root endodermal cells, and thus roots with higher or lower expression of SUB display altered suberin polymer deposition patterns and modified composition”
e5“While these changes did not interfere with Casparian strip formation”
Claim · model-written summary
Altered SUB expression had a substantial effect on root uptake capacity in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- higher or lower expression of SUBe4
- Outcome
- root uptake capacitye6
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Arabidopsis SUBERMAN (SUB) transcription factor controls the establishment of the root suberin lamellae”
e4“In Arabidopsis, SUB is expressed in patchy and continuous suberization root endodermal cells, and thus roots with higher or lower expression of SUB display altered suberin polymer deposition patterns and modified composition”
e6“they had a substantial effect on root uptake capacity, resulting in varied root and leaf ionomic phenotypes”
Claim · model-written summary
Altered SUB expression resulted in varied root and leaf ionomic phenotypes in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise1
- Outcome
- root and leaf ionomic phenotypese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Arabidopsis SUBERMAN (SUB) transcription factor controls the establishment of the root suberin lamellae”
e6“they had a substantial effect on root uptake capacity, resulting in varied root and leaf ionomic phenotypes”
Claim · model-written summary
SUB function impacts transcriptional networks associated with suberin, phenylpropanoids and lignin in Arabidopsis roots.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SUBe7
- Outcome
- transcriptional networks associated with suberin, phenylpropanoids, lignine7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Arabidopsis SUBERMAN (SUB) transcription factor controls the establishment of the root suberin lamellae”
e7“Gene expression profiling revealed that SUB function impacts transcriptional networks associated with suberin, phenylpropanoids, lignin, and cuticular lipid biosynthesis, as well as root transport activities, hormone signalling, and cell wall modification”
Claim · model-written summary
SUB function impacts cuticular lipid biosynthesis transcriptional networks in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SUBe7
- Outcome
- cuticular lipid biosynthesise7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Arabidopsis SUBERMAN (SUB) transcription factor controls the establishment of the root suberin lamellae”
e7“Gene expression profiling revealed that SUB function impacts transcriptional networks associated with suberin, phenylpropanoids, lignin, and cuticular lipid biosynthesis, as well as root transport activities, hormone signalling, and cell wall modification”
Claim · model-written summary
SUB function impacts transcriptional networks associated with root transport activities, hormone signalling and cell wall modification in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- SUBe7
- Outcome
- root transport activities, hormone signalling, and cell wall modificatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the Arabidopsis SUBERMAN (SUB) transcription factor controls the establishment of the root suberin lamellae”
e7“Gene expression profiling revealed that SUB function impacts transcriptional networks associated with suberin, phenylpropanoids, lignin, and cuticular lipid biosynthesis, as well as root transport activities, hormone signalling, and cell wall modification”
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See all 29 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.