Abstract
The jujube witches' broom (JWB) phytoplasma is associated with witches' broom, dwarfism, and smaller leaves in jujube, resulting in yield losses. In this study, eight putative JWB effector proteins were identified from potential mobile units of the JWB genome. Among them, Zaofeng6 induced witches' broom symptoms in Arabidopsis and jujube. Zaofeng6-overexpressing Arabidopsis and unrooted jujube transformants displayed witches' broom-like shoot proliferation. Transient expression of Zaofeng6 induced hypersensitive response like cell death and expression of hypersensitive response marker genes, like harpin-induced gene 1 (H1N1), and the pathogenesis-related genes PR1, PR2, and PR3 in transformed Nicotiana benthamiana leaves, suggesting that Zaofeng6 could be a virulence effector. Yeast two-hybrid library screening and bimolecular fluorescence complementation confirmed that Zaofeng6 interacts with ZjTCP7 through its first two α-helix domains in the cell nuclei. ZjTCP7 mRNA and protein abundance decreased in Zaofeng6 transgenic jujube seedlings. The expression of some genes in the strigolactone signaling pathway (ZjCCD7, ZjCCD8, and CYP711A1) were down-regulated in jujube shoots overexpressing Zaofeng6 and in zjtcp7 CRISPR/Cas9 mutants. Zaofeng6 induces shoot proliferation through decreased expression of ZjTCP7 at the transcriptional and translational levels.
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Claims 11
Claim · model-written summary
Eight putative JWB effector proteins were identified.
- Taxon
- Ziziphus jujuba
Ziziphus jujubat0 - Interactor
- JWBe0pathogen
- Outcome
- eight putative JWB effector proteinse0
- Value
- eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“eight putative JWB effector proteins were identified from potential mobile units of the JWB genome”
t0“Ziziphus jujuba”
Claim · model-written summary
Zaofeng6 induced witches’ broom symptoms in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Zaofeng6e0
- Gene
- Zaofeng6e0
- Outcome
- witches' broom symptomse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zaofeng6 induced witches' broom symptoms in Arabidopsis and jujube”
Claim · model-written summary
Zaofeng6 induced witches’ broom symptoms in jujube.
Not reportedComparator
Verbatim
e0“Zaofeng6 induced witches' broom symptoms in Arabidopsis and jujube”
Claim · model-written summary
Zaofeng6 overexpression caused shoot proliferation in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Zaofeng6-overexpressinge0
- Outcome
- witches' broom-like shoot proliferatione0
- Direction
- displayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zaofeng6-overexpressing Arabidopsis and unrooted jujube transformants displayed witches' broom-like shoot proliferation”
Claim · model-written summary
Zaofeng6 jujube transformants showed shoot proliferation.
Verbatim
e0“Zaofeng6-overexpressing Arabidopsis and unrooted jujube transformants displayed witches' broom-like shoot proliferation”
Claim · model-written summary
Transient Zaofeng6 expression induced hypersensitive-like cell death in Nicotiana benthamiana leaves.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Organ / stage
- leavese0
- Treatment
- Transient expression of Zaofeng6e0
- Outcome
- hypersensitive response like cell deathe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of Zaofeng6 induced hypersensitive response like cell death and expression of hypersensitive response marker genes, like harpin-induced gene 1 (H1N1), and the pathogenesis-related genes PR1, PR2, and PR3 in transformed Nicotiana benthamiana leaves”
Claim · model-written summary
Transient Zaofeng6 expression induced defense marker gene expression in Nicotiana benthamiana leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Organ / stage
- leavese0
- Treatment
- Transient expression of Zaofeng6e0
- Outcome
- expression of hypersensitive response marker genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of Zaofeng6 induced hypersensitive response like cell death and expression of hypersensitive response marker genes, like harpin-induced gene 1 (H1N1), and the pathogenesis-related genes PR1, PR2, and PR3 in transformed Nicotiana benthamiana leaves”
Claim · model-written summary
Zaofeng6 interacted with ZjTCP7 through its first two alpha-helix domains in nuclei.
- Taxon
- Ziziphus jujubaZiziphus jujubat0
- Gene
- ZjTCP7e0
- Outcome
- interacts with ZjTCP7e0
- Qualifier
- through its first two α-helix domains in the cell nucleie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zaofeng6 interacts with ZjTCP7 through its first two α-helix domains in the cell nuclei”
t0“Ziziphus jujuba”
Claim · model-written summary
ZjTCP7 mRNA and protein abundance decreased in Zaofeng6 transgenic jujube seedlings.
- Taxon
- jujubee0
- Organ / stage
- seedlingse0
- Treatment
- Zaofeng6 transgenice0
- Gene
- ZjTCP7e0
- Outcome
- ZjTCP7 mRNA and protein abundancee0
- Direction
- decreasede0
Not reportedComparator
Verbatim
e0“ZjTCP7 mRNA and protein abundance decreased in Zaofeng6 transgenic jujube seedlings”
Claim · model-written summary
Strigolactone signaling genes were downregulated in jujube shoots overexpressing Zaofeng6.
- Taxon
- jujubee0
- Organ / stage
- shootse0
- Treatment
- overexpressing Zaofeng6e0
- Gene
- ZjCCD7, ZjCCD8, and CYP711A1e0
- Outcome
- expression of some genes in the strigolactone signaling pathwaye0
- Direction
- down-regulatede0
Not reportedComparator
Verbatim
e0“The expression of some genes in the strigolactone signaling pathway (ZjCCD7, ZjCCD8, and CYP711A1) were down-regulated in jujube shoots overexpressing Zaofeng6 and in zjtcp7 CRISPR/Cas9 mutants”
Claim · model-written summary
Strigolactone signaling genes were downregulated in zjtcp7 mutants.
- Taxon
- jujubee0
- Treatment
- zjtcp7 CRISPR/Cas9 mutantse0
- Gene
- ZjCCD7, ZjCCD8, and CYP711A1e0
- Outcome
- expression of some genes in the strigolactone signaling pathwaye0
- Direction
- down-regulatede0
Not reportedComparator
Verbatim
e0“The expression of some genes in the strigolactone signaling pathway (ZjCCD7, ZjCCD8, and CYP711A1) were down-regulated in jujube shoots overexpressing Zaofeng6 and in zjtcp7 CRISPR/Cas9 mutants”
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See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.