Abstract
A pot experiment including four treatments,i.e.CK(neither urea nor cattle manure was applied),CF(100% of nitrogen was provided by urea),CM(100% of nitrogen was provided by cattle manure),and CM+CF(50% and 50% of nitrogen was provided by cattle manure and urea,respectively) was conducted to evaluate the effects of cattle manure co-applied with inorganic fertilizer on root morphological characteristics and microorganism population,enzyme activtiy in the rhizosphere soil of Platycladus orientalis.The results indicated that compared with CF treatment,CM+CF treatment significantly increased the root surface area,specific root surface area,total root length,root tip number and root activity,showing 110.01%,59.27% and 28.54% increases in root surface area,respectively,in comparison to the treatments of CK,CF and CM.At the same time,the microbial populaton,microbial biomass carbon(MBC) and microbial biomass nitrogen(MBN) in CM+CF treatment was also increased,demonstrating 62.22% and 45.35% increases in MBC and MBN contents compared to the CF treatment,respectively.The microbial biodiversity indices and microbial population had the same variation tendency between CM and CM+CF treatments,while the CK and CF treatments had the opposite variation tendency.In additon,the activity of urea enzyme,catalase enzyme,polyphenol oxidase enzyme and invertase enzyme in CM+CF treatment was still improved,and the increase amplitude of polyphenol oxidase enzyme was the maximum.In contrast to CM+CF treatment,the CM treatment had less effect on the rhizosphere environment of Platycladus orientalis.In conclusion,cattle manure co-applied inorganic fertilizer can be beneficial to increase the number of soil microorganism and enzyme activities,and create a good miro-ecological environment for the growth of Platycladus orientalis.
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Claims 13
Claim · model-written summary
Combined manure and urea significantly increased root surface area, specific root surface area and total root length relative to urea.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Treatment
- CM+CF treatmente0
- Outcome
- root surface area,specific root surface area,total root lengthe0
- Direction
- increasede0
- Comparator
- CF treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“compared with CF treatment,CM+CF treatment significantly increased the root surface area,specific root surface area,total root length”
e1“rhizosphere soil of Platycladus orientalis”
Claim · model-written summary
Combined manure and urea significantly increased root tip number and activity relative to urea.
- Taxon
- Platycladus orientalis
Platycladus orientalise2 - Treatment
- CM+CF treatmente1
- Outcome
- root tip number and root activitye0
- Direction
- increasede1
- Comparator
- CF treatmente1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“root tip number and root activity”
e1“compared with CF treatment,CM+CF treatment significantly increased the root surface area”
e2“rhizosphere soil of Platycladus orientalis”
Claim · model-written summary
Combined treatment increased root surface area by 110.01% against CK.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Treatment
- CM+CF treatmente2
- Outcome
- root surface areae0
- Direction
- increasese0
- Value
- 110.01%e0
- Comparator
- CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“110.01%,59.27% and 28.54% increases in root surface area,respectively,in comparison to the treatments of CK,CF and CM”
e1“rhizosphere soil of Platycladus orientalis”
e2“CM+CF treatment significantly increased the root surface area”
Claim · model-written summary
Combined treatment increased root surface area by 59.27% against CF.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Treatment
- CM+CF treatmente2
- Outcome
- root surface areae0
- Direction
- increasese0
- Value
- 59.27%e0
- Comparator
- CFe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“110.01%,59.27% and 28.54% increases in root surface area,respectively,in comparison to the treatments of CK,CF and CM”
e1“rhizosphere soil of Platycladus orientalis”
e2“CM+CF treatment significantly increased the root surface area”
Claim · model-written summary
Combined treatment increased root surface area by 28.54% against CM.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Treatment
- CM+CF treatmente2
- Outcome
- root surface areae0
- Direction
- increasese0
- Value
- 28.54%e0
- Comparator
- CMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“110.01%,59.27% and 28.54% increases in root surface area,respectively,in comparison to the treatments of CK,CF and CM”
e1“rhizosphere soil of Platycladus orientalis”
e2“CM+CF treatment significantly increased the root surface area”
Claim · model-written summary
Combined treatment increased rhizosphere microbial population.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Treatment
- CM+CF treatmente0
- Outcome
- microbial populatone0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the microbial populaton,microbial biomass carbon(MBC) and microbial biomass nitrogen(MBN) in CM+CF treatment was also increased”
e1“rhizosphere soil of Platycladus orientalis”
Claim · model-written summary
Combined treatment increased microbial biomass carbon by 62.22% over CF.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Treatment
- CM+CF treatmente2
- Outcome
- MBCe0
- Direction
- increasese0
- Value
- 62.22%e0
- Comparator
- CF treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“62.22% and 45.35% increases in MBC and MBN contents compared to the CF treatment,respectively”
e1“rhizosphere soil of Platycladus orientalis”
e2“CM+CF treatment was also increased”
Claim · model-written summary
Combined treatment increased microbial biomass nitrogen by 45.35% over CF.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Treatment
- CM+CF treatmente2
- Outcome
- MBNe0
- Direction
- increasese0
- Value
- 45.35%e0
- Comparator
- CF treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“62.22% and 45.35% increases in MBC and MBN contents compared to the CF treatment,respectively”
e1“rhizosphere soil of Platycladus orientalis”
e2“CM+CF treatment was also increased”
Claim · model-written summary
Microbial diversity indices and populations varied similarly under CM and CM+CF but oppositely under CK and CF.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Outcome
- microbial biodiversity indices and microbial populatione0
- Comparator
- CK and CF treatmentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The microbial biodiversity indices and microbial population had the same variation tendency between CM and CM+CF treatments,while the CK and CF treatments had the opposite variation tendency”
e1“rhizosphere soil of Platycladus orientalis”
Claim · model-written summary
Combined treatment improved rhizosphere urease, catalase and polyphenol oxidase activity.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Treatment
- CM+CF treatmente0
- Outcome
- activity of urea enzyme,catalase enzyme,polyphenol oxidase enzymee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the activity of urea enzyme,catalase enzyme,polyphenol oxidase enzyme and invertase enzyme in CM+CF treatment was still improved”
e1“rhizosphere soil of Platycladus orientalis”
Claim · model-written summary
Combined treatment improved rhizosphere invertase activity.
- Taxon
- Platycladus orientalis
Platycladus orientalise1 - Treatment
- CM+CF treatmente0
- Outcome
- invertase enzymee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“invertase enzyme in CM+CF treatment was still improved”
e1“rhizosphere soil of Platycladus orientalis”
Claim · model-written summary
Polyphenol oxidase activity had the largest increase under combined treatment.
- Taxon
- Platycladus orientalis
Platycladus orientalise2 - Treatment
- CM+CF treatmente1
- Outcome
- polyphenol oxidase enzymee0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“the increase amplitude of polyphenol oxidase enzyme was the maximum”
e1“CM+CF treatment was still improved”
e2“rhizosphere soil of Platycladus orientalis”
Claim · model-written summary
Manure alone affected the rhizosphere less than combined treatment.
- Taxon
- Platycladus orientalis
Platycladus orientalise0 - Treatment
- CM treatmente0
- Outcome
- rhizosphere environmente0
- Direction
- lesse0
- Comparator
- CM+CF treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“In contrast to CM+CF treatment,the CM treatment had less effect on the rhizosphere environment of Platycladus orientalis”