Abstract
In the previous study,3 virulence mutants of wheat stripe rust pathogen,Puccinia striiformis,with GUS insertion were obtained.In this study,expression of GUS gene was analyzed by histochemical staining with X-Gluc,and confirmed by specific PCR and Southern blot.The results indicated that the alien gene GUS was integrated and could inherit stably in the stripe rust genome.The pathogenicity of the 3 mutants on 17 wheat stripe rust differentials and 37 extension cultivars was different from that of the wild isolate.The inserted alien fragment resulted in the pathogenic variation of the mutants.The insertion sites of foreign gene were relevant to the virulent gene of Puccinia striiformis.This study provides a basis for the cloning of the avirulent genes.
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Claims 5
Claim · model-written summary
The inserted GUS gene was integrated into the stripe rust genome.
- Taxon
- wheat
Triticum aestivumt0 - Interactor
- stripe rustt0pathogen
- Gene
- GUSe0
- Outcome
- integratede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the alien gene GUS was integrated and could inherit stably in the stripe rust genome”
t0“wheat stripe rust”
Claim · model-written summary
The GUS insertion was stably inherited in the stripe rust genome.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- stripe rustt0pathogen
- Gene
- GUSe0
- Outcome
- inherit stablye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the alien gene GUS was integrated and could inherit stably in the stripe rust genome”
t0“wheat stripe rust”
Claim · model-written summary
Three mutants differed from the wild isolate in pathogenicity on 17 wheat stripe rust differentials and 37 extension cultivars.
- Taxon
- wheatTriticum aestivume0
- Material
- wheat stripe rust differentialse0
- Interactor
- stripe rustt0pathogen
- Outcome
- pathogenicitye0
- Direction
- differente0
- Comparator
- wild isolatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pathogenicity of the 3 mutants on 17 wheat stripe rust differentials and 37 extension cultivars was different from that of the wild isolate”
t0“stripe rust”
Claim · model-written summary
The inserted fragment caused pathogenic variation in the mutants.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- stripe rustt0pathogen
- Outcome
- pathogenic variation of the mutantse0
- Qualifier
- inserted alien fragmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inserted alien fragment resulted in the pathogenic variation of the mutants”
t0“wheat stripe rust”
Claim · model-written summary
Foreign gene insertion sites were associated with a virulence gene of Puccinia striiformis.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- Puccinia striiformis
Puccinia striiformise0pathogen - Gene
- virulent gene of Puccinia striiformise0
- Outcome
- insertion sites of foreign genee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The insertion sites of foreign gene were relevant to the virulent gene of Puccinia striiformis”
t0“wheat”