Abstract
In order to verify the efficiency of DONtest-HPLC in breeding wheat with low DON,and to reveal the variation of DON content in wheat,8 cultivars with different resistance to fusarium head blight were used for analysis when fusarium head blight epidemic severely occurred in the middle and lower stretches of Yangtze River in 2003.The results indicated that DONtest-HPLC was a kind of quick and accurate method in detection of DON content with a recovery rate 78.2% in average.Based on the preliminary investigation,DON content varied in wheat with different resistance.Of the three high resistance cultivars,Wangshuibai showed the lowest amount,while in the medium resistance type,Yangmai158 was the lowest.This variation was further confirmed by test of single floret inoculation with the high DON-yielding isolates of Fusarium graminearum.The results also demonstrated that 12 samples collected from 4 regions of Jiangsu Province were totally contaminated in different degree with DON content ranged from 0.175 mg·kg~-1 to 7.239 mg·kg~-1.Percentage of diseased grains significantly correlated with DON content,which might be an effective indirect parameter in rough selection of wheat with low DON content.
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Claims 8
Claim · model-written summary
DONtest-HPLC detected DON with 78.2% average recovery.
- Taxon
- common wheat
Triticum aestivumt0 - Outcome
- recovery ratee0
- Value
- 78.2%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DONtest-HPLC was a kind of quick and accurate method in detection of DON content with a recovery rate 78.2% in average”
t0“common wheat”
Claim · model-written summary
DON content varied among wheat with different resistance.
- Taxon
- wheatTriticum aestivume0
- Material
- wheat with different resistancee0
- Outcome
- DON contente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DON content varied in wheat with different resistance”
Claim · model-written summary
Wangshuibai had the lowest DON amount among highly resistant cultivars.
- Taxon
- wheatTriticum aestivumt0
- Material
- Wangshuibaie0
- Outcome
- DON contente1
- Direction
- loweste0
- Comparator
- three high resistance cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Of the three high resistance cultivars,Wangshuibai showed the lowest amount”
e1“DON content varied in wheat with different resistance”
t0“wheat”
Claim · model-written summary
Yangmai158 had the lowest DON among medium resistance cultivars.
- Taxon
- wheatTriticum aestivumt0
- Material
- Yangmai158e0
- Outcome
- DON contente1
- Direction
- loweste0
- Comparator
- medium resistance typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the medium resistance type,Yangmai158 was the lowest”
e1“DON content varied in wheat with different resistance”
t0“wheat”
Claim · model-written summary
Single floret inoculation with Fusarium graminearum confirmed DON variation.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- single floret inoculatione0
- Interactor
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Outcome
- This variatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“This variation was further confirmed by test of single floret inoculation with the high DON-yielding isolates of Fusarium graminearum”
t0“wheat”
Claim · model-written summary
All 12 wheat samples from four Jiangsu regions were contaminated with DON at 0.175 to 7.239 mg per kg.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- DON contente0
- Value
- 0.175 mg·kg~-1 to 7.239 mg·kg~-1e0
- Qualifier
- 12 samplese0
- Setting
- Jiangsu Provincee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“12 samples collected from 4 regions of Jiangsu Province were totally contaminated in different degree with DON content ranged from 0.175 mg·kg~-1 to 7.239 mg·kg~-1”
t0“wheat”
Claim · model-written summary
Percentage of diseased grains correlated significantly with DON content.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- DON contente0
- Comparator
- Percentage of diseased grainse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Percentage of diseased grains significantly correlated with DON content”
t0“wheat”
Claim · model-written summary
The authors propose diseased grain percentage as an indirect selection parameter for low DON wheat.
- Taxon
- wheatTriticum aestivume0
- Outcome
- effective indirect parameter in rough selection of wheat with low DON contente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Percentage of diseased grains significantly correlated with DON content,which might be an effective indirect parameter in rough selection of wheat with low DON content”