Abstract
Based on the property of Fe(Ⅲ) with thiocyanate and tannin can occur specific reaction to form a stable three element metal ionic compounds,a new method for the determination of tannin in sorghum grain sorghum was established. The results showed that the color of liquid changed significantly after the reaction of ferric thiocyanate and tannin,and there was an obvious absorption peak at 575nm. Under the optimized conditions, the concentration of tannins between 0. 001 ~0. 06g / L was obeyed the Lambert-Beer ' s law. Correspondingly,the regression equation was Y=13.664X+0.0389,and the correlation coefficient R2was 0.9993. The average recovery of this new method was in the range of 95.77%~101.60%,and the RSD1%. This new method was used to determined tannins content in sorghum samples,and the results were consisted with national standard test method(GBT 15686-2008). The method,which is simple,rapid,high sensitivity,specificity and strong anti-interference ability,can be applied to the sorghum tannins rapid detection and identification of sorghum varieties.
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Claims 9
Claim · model-written summary
The reaction produced a significant liquid color change.
SorghumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the color of liquid changed significantly after the reaction of ferric thiocyanate and tannin”
t0“sorghum grain”
Claim · model-written summary
The reaction had an absorption peak at 575nm.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was an obvious absorption peak at 575nm”
t0“sorghum grain”
Claim · model-written summary
Tannin concentration followed Lambert-Beer law from 0. 001 to 0. 06g / L.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the concentration of tannins between 0. 001 ~0. 06g / L was obeyed the Lambert-Beer ' s law”
t0“sorghum grain”
Claim · model-written summary
The calibration regression equation was Y=13.664X+0.0389.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the regression equation was Y=13.664X+0.0389”
t0“sorghum grain”
Claim · model-written summary
The calibration correlation coefficient was R2 0.9993.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the correlation coefficient R2was 0.9993”
t0“sorghum grain”
Claim · model-written summary
Average recovery ranged from 95.77% to 101.60%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The average recovery of this new method was in the range of 95.77%~101.60%”
t0“sorghum grain”
Claim · model-written summary
The method had RSD1%.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the RSD1%”
t0“sorghum grain”
Claim · model-written summary
Sorghum tannin results agreed with the national standard method.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tannins content in sorghum samples,and the results were consisted with national standard test method(GBT 15686-2008)”
Claim · model-written summary
The authors propose the method for rapid detection of sorghum tannins and identification of varieties.
- Outcome
- sorghum tannins rapid detection and identification of sorghum varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“can be applied to the sorghum tannins rapid detection and identification of sorghum varieties”