Paper · 2010 · Journal of Jilin University

Effect of extrinsic regulators on growth and ginsenoside content of hairy roots of Panax ginseng

Jin Shi-kun

6
claims
15
verbatim quotes
1
citations
1
research areas
—
Not open access

Abstract

The effect of adding 2,4-D,NAA,IBA,MJA and SA to cell suspension cultures on the growth,content of total saponins and ginsenoside Rb1of Panax ginsenghairy roots were investigated.Results show that three phytohormones could improve the growth of the hairy roots and promote saponin accumulation in different degrees.Addition of IBA at the level of 0.5mg/L could significantly promote the growth(50.73g/L per month) ,and saponin accumulation(saponin content of 3.31%) of the hairy roots and raise remarkably the proportion of ginsenoside Rb1(Rb1content of 2.293%) in the total saponins.Other two elicitors could not significantly promote the growth,but,with suitable concentrations,could promote saponin accumulation of the hairy roots.The two elicitors have good synergistic effect on the saponin accumulation.With the combination of 0.001mmol/L MJA and 0.001mmol/L SA,the accumulation of total ginsenosides of the hairy roots is higher than that with either elicitor alone.

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Claims 6

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Adding IBA at 0.5 mg/L significantly promoted the growth of Panax ginseng hairy roots (50.73 g/L per month).

Taxon
Panax ginsengPanax ginsengt0
Treatment
Addition of IBA at the level of 0.5mg/Le1
Outcome
growthe1
Direction
promotee1
Value
50.73g/L per monthe1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“hairy roots of Panax ginseng”

✓ located verbatim in the source · title, characters 68–96 · show in text ↑
e1

“Addition of IBA at the level of 0.5mg/L could significantly promote the growth(50.73g/L per month) ,and saponin accumulation(saponin content of 3.31%) of the hairy roots”

✓ located verbatim in the source · abstract, characters 315–484 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Adding IBA at 0.5 mg/L significantly promoted saponin accumulation in Panax ginseng hairy roots (saponin content 3.31%).

Taxon
Panax ginsengPanax ginsengt0
Treatment
Addition of IBA at the level of 0.5mg/Le1
Outcome
saponin accumulatione1
Direction
promotee1
Value
3.31%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“hairy roots of Panax ginseng”

✓ located verbatim in the source · title, characters 68–96 · show in text ↑
e1

“Addition of IBA at the level of 0.5mg/L could significantly promote the growth(50.73g/L per month) ,and saponin accumulation(saponin content of 3.31%) of the hairy roots”

✓ located verbatim in the source · abstract, characters 315–484 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding IBA at 0.5 mg/L raised the proportion of ginsenoside Rb1 in total saponins (Rb1 content 2.293%).

Taxon
Panax ginsengPanax ginsengt0
Treatment
Addition of IBA at the level of 0.5mg/Le1
Outcome
proportion of ginsenoside Rb1e2
Direction
raisee2
Value
2.293%e2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“hairy roots of Panax ginseng”

✓ located verbatim in the source · title, characters 68–96 · show in text ↑
e1

“Addition of IBA at the level of 0.5mg/L could significantly promote the growth(50.73g/L per month) ,and saponin accumulation(saponin content of 3.31%) of the hairy roots”

✓ located verbatim in the source · abstract, characters 315–484 · show in text ↑
e2

“raise remarkably the proportion of ginsenoside Rb1(Rb1content of 2.293%) in the total saponins”

✓ located verbatim in the source · abstract, characters 489–583 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The other two elicitors (MJA and SA) did not significantly promote growth of Panax ginseng hairy roots.

Taxon
Panax ginsengPanax ginsengt0
Treatment
MJA and SAe0
Outcome
growthe3
Direction
could not significantly promotee3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“hairy roots of Panax ginseng”

✓ located verbatim in the source · title, characters 68–96 · show in text ↑
e0

“2,4-D,NAA,IBA,MJA and SA to cell suspension cultures”

✓ located verbatim in the source · abstract, characters 21–73 · show in text ↑
e3

“Other two elicitors could not significantly promote the growth,but,with suitable concentrations,could promote saponin accumulation of the hairy roots”

✓ located verbatim in the source · abstract, characters 584–733 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At suitable concentrations, the other two elicitors (MJA and SA) promoted saponin accumulation in Panax ginseng hairy roots.

Taxon
Panax ginsengPanax ginsengt0
Treatment
MJA and SAe0
Outcome
saponin accumulatione3
Direction
could promotee3
Qualifier
with suitable concentrationse3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“hairy roots of Panax ginseng”

✓ located verbatim in the source · title, characters 68–96 · show in text ↑
e0

“2,4-D,NAA,IBA,MJA and SA to cell suspension cultures”

✓ located verbatim in the source · abstract, characters 21–73 · show in text ↑
e3

“Other two elicitors could not significantly promote the growth,but,with suitable concentrations,could promote saponin accumulation of the hairy roots”

✓ located verbatim in the source · abstract, characters 584–733 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The combination of 0.001 mmol/L MJA and 0.001 mmol/L SA gave higher total ginsenoside accumulation in hairy roots than either elicitor alone.

Taxon
Panax ginsengPanax ginsengt0
Treatment
combination of 0.001mmol/L MJA and 0.001mmol/L SAe4
Outcome
accumulation of total ginsenosidese4
Direction
highere4
Comparator
either elicitor alonee4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

t0

“hairy roots of Panax ginseng”

✓ located verbatim in the source · title, characters 68–96 · show in text ↑
e4

“With the combination of 0.001mmol/L MJA and 0.001mmol/L SA,the accumulation of total ginsenosides of the hairy roots is higher than that with either elicitor alone”

✓ located verbatim in the source · abstract, characters 809–972 · show in text ↑

salicylic acid · molecular: metabolites · Panax ginseng (ginseng)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

indole-3-butyric acid · growth · Panax ginseng (ginseng)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.