Abstract
The research concerned of the regeneration of plants from embryos obtained from anther cultures of ginseng (Panax ginseng C. A. Meyer). The aim was to determine the influence of the regeneration medium on the efficiency of the regeneration process. We conducted to determine the optimum conditions such as cold pretreatment, plant growth regulators and carbon sources on anther culture of P. ginseng. Highest callus formation rate was obtained when flower buds pretreated at $4^{\circ}C$ for 1 day. Among the treated growth regulators with various degrees of concentration in Murashige and Skoog's (MS) medium, 4.53 ${\mu}M$ of 2.4-dichlorophenoxyacetic acid and 4.44 ${\mu}M$ of 6-benzylaminopurine gives the most responsive callus with the frequency of 73.89% and 129.53 g of fresh weight. When we used 3-9% of sucrose and maltose among the different kinds and various concentrations of carbohydrates, callus was formed highest 67.29% in the medium with 3% of sucrose. Shoots induced from callus supplemented with 28.9 ${\mu}M$ of gibberellic acid and rooted in Gamborg's B5 medium supplemented with 14.7 ${\mu}M$ of indole-3-butyric acid.
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Claims 6
Claim · model-written summary
Cold pretreatment at 4°C for one day gave the highest callus formation.
- Taxon
- Panax ginseng
Panax ginsengt0 - Organ / stage
- flower budse0
- Treatment
- pretreated at $4^{\circ}C$ for 1 daye0
- Outcome
- callus formation ratee0
- Direction
- Higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest callus formation rate was obtained when flower buds pretreated at $4^{\circ}C$ for 1 day”
t0“Panax ginseng”
Claim · model-written summary
The auxin and cytokinin combination yielded 73.89% responsive callus.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- 4.53 ${\mu}M$ of 2.4-dichlorophenoxyacetic acid and 4.44 ${\mu}M$ of 6-benzylaminopurinee0
- Outcome
- responsive calluse0
- Direction
- moste0
- Value
- 73.89%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“4.53 ${\mu}M$ of 2.4-dichlorophenoxyacetic acid and 4.44 ${\mu}M$ of 6-benzylaminopurine gives the most responsive callus with the frequency of 73.89% and 129.53 g of fresh weight”
t0“Panax ginseng”
Claim · model-written summary
The same regulator combination produced 129.53 g fresh weight.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- 4.53 ${\mu}M$ of 2.4-dichlorophenoxyacetic acid and 4.44 ${\mu}M$ of 6-benzylaminopurinee1
- Outcome
- fresh weighte0
- Value
- 129.53 ge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“129.53 g of fresh weight”
t0“Panax ginseng”
e1“4.53 ${\mu}M$ of 2.4-dichlorophenoxyacetic acid and 4.44 ${\mu}M$ of 6-benzylaminopurine”
Claim · model-written summary
Three percent sucrose gave the highest callus formation frequency, 67.29%.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- 3% of sucrosee0
- Outcome
- callus was formede0
- Direction
- higheste0
- Value
- 67.29%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“callus was formed highest 67.29% in the medium with 3% of sucrose”
t0“Panax ginseng”
Claim · model-written summary
Gibberellic acid induced shoots from callus.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- 28.9 ${\mu}M$ of gibberellic acide0
- Outcome
- Shoots induced from calluse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoots induced from callus supplemented with 28.9 ${\mu}M$ of gibberellic acid”
t0“Panax ginseng”
Claim · model-written summary
Indole-3-butyric acid in Gamborg medium rooted shoots.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- Gamborg's B5 medium supplemented with 14.7 ${\mu}M$ of indole-3-butyric acide0
- Outcome
- rootede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rooted in Gamborg's B5 medium supplemented with 14.7 ${\mu}M$ of indole-3-butyric acid”
t0“Panax ginseng”
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.