Paper · 2005 · Current Science

Optimization of organic nutrients for ginseng hairy roots production in large-scale bioreactors

Ganapathy Sivakumar; K. W. Yu; Eun-Joo Hahn

4
claims
8
verbatim quotes
69
citations
1
research areas
—
Not open access

Abstract

Macro and micro-elements, carbon sources and elicitors play a central role during Panax ginseng hairy root growth, biomass and ginsenoside production and also influence numerous root developmental stages in large scale bioreactor culture. Due to the lack of biosynthetic nutrients and signalling-inducing nutrients, the regulatory role of mineral elements is not well understood. We have genetically engineered Agrobacterium rhizogenes KCTC 2703 to have the rol C gene in T -DNA of ginseng calluses that produce hairy roots. A normal MS medium nutrient in the bioreactor gave low hairy roots growth, biomass and ginsenoside content, indicating that an optimal mineral element ratio is required to increase hairy root growth and biomass. In addition, elicitors also play an important role in increas ing ginsenoside production. These results suggest that mineral elements are an important regulatory factor in hairy root growth and biomass, while elicitors decrease biomass and increase ginsenoside content.

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Claims 4

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Normal MS medium nutrient in the bioreactor gave lower hairy root growth, biomass and ginsenoside content than an optimal mineral element ratio.

Taxon
Panax ginsengPanax ginsenge1
Treatment
normal MS medium nutriente2
Outcome
hairy roots growth, biomass and ginsenoside contente2
Direction
lowe2
Comparator
optimal mineral element ratioe2
Setting
bioreactore2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Macro and micro-elements, carbon sources and elicitors play a central role during Panax ginseng hairy root growth, biomass and ginsenoside production”

✓ located verbatim in the source · abstract, characters 0–149 · show in text ↑
e2

“A normal MS medium nutrient in the bioreactor gave low hairy roots growth, biomass and ginsenoside content, indicating that an optimal mineral element ratio is required to increase hairy root growth and biomass”

✓ located verbatim in the source · abstract, characters 525–735 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Elicitors decreased biomass of Panax ginseng hairy roots.

Taxon
Panax ginsengPanax ginsenge1
Treatment
elicitorse4
Outcome
biomasse4
Direction
decreasee4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Macro and micro-elements, carbon sources and elicitors play a central role during Panax ginseng hairy root growth, biomass and ginsenoside production”

✓ located verbatim in the source · abstract, characters 0–149 · show in text ↑
e4

“while elicitors decrease biomass and increase ginsenoside content”

✓ located verbatim in the source · abstract, characters 940–1005 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Elicitors increased ginsenoside content of Panax ginseng hairy roots.

Taxon
Panax ginsengPanax ginsenge1
Treatment
elicitorse4
Outcome
ginsenoside contente4
Direction
increasee4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Macro and micro-elements, carbon sources and elicitors play a central role during Panax ginseng hairy root growth, biomass and ginsenoside production”

✓ located verbatim in the source · abstract, characters 0–149 · show in text ↑
e4

“while elicitors decrease biomass and increase ginsenoside content”

✓ located verbatim in the source · abstract, characters 940–1005 · show in text ↑
Associationauthor interpretationoriginal resultmeasured

Claim · model-written summary

Mineral elements are suggested to be an important regulatory factor in hairy root growth and biomass of Panax ginseng.

Taxon
Panax ginsengPanax ginsenge1
Treatment
mineral elementse3
Outcome
hairy root growth and biomasse3

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Panax ginseng
Panax ginseng · TaxonFloraFarm GmbH / Katharina Lohrie · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e1

“Macro and micro-elements, carbon sources and elicitors play a central role during Panax ginseng hairy root growth, biomass and ginsenoside production”

✓ located verbatim in the source · abstract, characters 0–149 · show in text ↑
e3

“These results suggest that mineral elements are an important regulatory factor in hairy root growth and biomass”

✓ located verbatim in the source · abstract, characters 827–938 · show in text ↑

resistance inducer · growth · Panax ginseng (ginseng)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

in vitro · growth · Panax ginseng (ginseng)Positive and negative results

11 papers: 10 positive, 1 negative or no effect

10 in the opposite direction

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

resistance inducer · molecular: metabolites · Panax ginseng (ginseng)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.