Abstract
Ginsenoside is the most important secondary metabolite of ginseng. Natural sources of wild ginseng have been overexploited. Although root culture could reduce the length of the growth cycle of ginseng, the number of ginsenosides is fewer and their contents are lower in adventitious roots of ginseng than that in ginseng cultivated in the field. In this study, we investigated the effects of endophytic bacterial elicitors on biomass and ginsenoside production in adventitious roots cultures of Panax ginseng. Endophyte LB 5-3 as an elicitor could increase biomass and ginsenoside accumulation in ginseng adventitious root culture. After 6 days elicitation with a 10.0 mL of strain LB 5-3, the content of total ginsenoside was 2.026 mg g−1 which was four times more than that in unchallenged roots. The combination of methyl jasmonate and strain LB 5-3 had a negative effect on ginseng adventitious root growth and ginsenoside production. The genomic DNA of strain LB 5-3 was sequenced, and was found to be most closely related to Bacillus altitudinis (KX230132.1). The challenged ginseng adventitious root extracts exerted inhibitory effect against the HepG2 cells, which IC50 value was 0.94 mg mL−1.
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Claims 7
Claim · model-written summary
Endophyte LB 5-3 increased ginseng adventitious root biomass.
- Taxon
- ginseng
Panax ginsenge0 - Organ / stage
- adventitious roote0
- Treatment
- Endophyte LB 5-3 as an elicitore0
- Interactor
- Endophyte LB 5-3e0symbiont
- Outcome
- biomasse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Endophyte LB 5-3 as an elicitor could increase biomass and ginsenoside accumulation in ginseng adventitious root culture.”
Claim · model-written summary
Endophyte LB 5-3 increased ginsenoside accumulation in ginseng adventitious roots.
- Taxon
- ginsengPanax ginsenge0
- Organ / stage
- adventitious roote0
- Treatment
- Endophyte LB 5-3 as an elicitore0
- Interactor
- Endophyte LB 5-3e0symbiont
- Outcome
- ginsenoside accumulatione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Endophyte LB 5-3 as an elicitor could increase biomass and ginsenoside accumulation in ginseng adventitious root culture.”
Claim · model-written summary
After six days with 10.0 mL of LB 5-3, total ginsenoside reached 2.026 mg g−1, four times the unchallenged root level.
- Taxon
- ginsengPanax ginsenge0
- Treatment
- 10.0 mL of strain LB 5-3e1
- Interactor
- strain LB 5-3e1symbiont
- Outcome
- content of total ginsenosidee1
- Direction
- four times moree1
- Value
- 2.026 mg g−1e1
- Comparator
- unchallenged rootse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ginseng adventitious root culture”
e1“After 6 days elicitation with a 10.0 mL of strain LB 5-3, the content of total ginsenoside was 2.026 mg g−1 which was four times more than that in unchallenged roots.”
Claim · model-written summary
Combining methyl jasmonate and LB 5-3 negatively affected ginseng adventitious root growth.
- Taxon
- ginsengPanax ginsenge0
- Treatment
- combination of methyl jasmonate and strain LB 5-3e0
- Interactor
- strain LB 5-3e0symbiont
- Outcome
- adventitious root growthe0
- Direction
- negative effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combination of methyl jasmonate and strain LB 5-3 had a negative effect on ginseng adventitious root growth and ginsenoside production.”
Claim · model-written summary
Combining methyl jasmonate and LB 5-3 negatively affected ginseng ginsenoside production.
- Taxon
- ginsengPanax ginsenge0
- Treatment
- combination of methyl jasmonate and strain LB 5-3e0
- Interactor
- strain LB 5-3e0symbiont
- Outcome
- ginsenoside productione0
- Direction
- negative effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The combination of methyl jasmonate and strain LB 5-3 had a negative effect on ginseng adventitious root growth and ginsenoside production.”
Claim · model-written summary
Sequenced LB 5-3 DNA was most closely related to Bacillus altitudinis.
- Taxon
- ginsengPanax ginsenge0
- Interactor
- strain LB 5-3e1symbiont
- Outcome
- strain LB 5-3 was sequenced, and was found to be most closely related to Bacillus altitudinise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Endophyte LB 5-3 as an elicitor could increase biomass and ginsenoside accumulation in ginseng adventitious root culture.”
e1“The genomic DNA of strain LB 5-3 was sequenced, and was found to be most closely related to Bacillus altitudinis (KX230132.1).”
Claim · model-written summary
Challenged ginseng root extracts inhibited HepG2 cells with IC50 of 0.94 mg mL−1.
- Taxon
- ginsengPanax ginsenge0
- Organ / stage
- adventitious roote0
- Outcome
- inhibitory effect against the HepG2 cellse0
- Value
- 0.94 mg mL−1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The challenged ginseng adventitious root extracts exerted inhibitory effect against the HepG2 cells, which IC50 value was 0.94 mg mL−1.”
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