Abstract
OBJECTIVE: To optimize the culture condition of adventitious roots of Panax ginseng. METHOD: The adventitious roots were obtained through tissue culture by manipulation of inoculum, various sucrose concentrations and salt strength. The contents of ginsenosides Re, Rb1 and Rg1 were determined by HPLC while the contents of polysaccharides were determined by ultraviolet spectrophotometry. RESULT: The multiplication of adventitious roots reached the peak when the inoculum was 20 g x L(-1). The effects of sucrose concentration and salt strength on adventitious roots were observed. The contents of polysaccharides were higher when the medium contained more sucrose. 40 g x L(-1) sucrose was favorable for roots growth and biosynthesis of Re, while 30 g x L(-1) was favorable for the biosynthesis of Rb1 and Rg1. 3/4MS medium was benefit for the growth of adventitious roots and the biosynthesis of ginsenosides. The contents of polysaccharides were decreased with the increase of salt strength. CONCLUSION: The results showed that inoculum, various sucrose concentrations and salt strength have significant influences on adventitious roots growth, secondary metabolite and polysaccharide synthesis in P. ginseng.
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Claims 6
Claim · model-written summary
Multiplication of adventitious roots of Panax ginseng peaked at an inoculum of 20 g x L(-1).
- Taxon
- Panax ginseng
Panax ginsengt0 - Treatment
- inoculume0
- Outcome
- multiplication of adventitious rootse0
- Direction
- increaseinferrede0
- Value
- 20 g x L(-1)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adventitious roots culture in Panax ginseng”
e0“The multiplication of adventitious roots reached the peak when the inoculum was 20 g x L(-1)”
Claim · model-written summary
40 g x L(-1) sucrose was favorable for root growth and ginsenoside Re biosynthesis in Panax ginseng adventitious roots.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- sucrosee1
- Outcome
- roots growth and biosynthesis of Ree1
- Direction
- increaseinferrede1
- Value
- 40 g x L(-1)e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adventitious roots culture in Panax ginseng”
e1“40 g x L(-1) sucrose was favorable for roots growth and biosynthesis of Re”
Claim · model-written summary
30 g x L(-1) sucrose was favorable for Rb1 and Rg1 biosynthesis in Panax ginseng adventitious roots.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- sucrosee1
- Outcome
- biosynthesis of Rb1 and Rg1e2
- Direction
- increaseinferrede2
- Value
- 30 g x L(-1)e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adventitious roots culture in Panax ginseng”
e1“40 g x L(-1) sucrose was favorable for roots growth and biosynthesis of Re”
e2“30 g x L(-1) was favorable for the biosynthesis of Rb1 and Rg1”
Claim · model-written summary
Polysaccharide contents of Panax ginseng adventitious roots were higher when the medium contained more sucrose.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- sucrosee3
- Outcome
- contents of polysaccharidese3
- Direction
- highere3
- Qualifier
- when the medium contained more sucrosee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adventitious roots culture in Panax ginseng”
e3“The contents of polysaccharides were higher when the medium contained more sucrose”
Claim · model-written summary
3/4MS medium was favorable for the growth of Panax ginseng adventitious roots and ginsenoside biosynthesis.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- 3/4MS mediume4
- Outcome
- growth of adventitious roots and the biosynthesis of ginsenosidese4
- Direction
- increaseinferrede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adventitious roots culture in Panax ginseng”
e4“3/4MS medium was benefit for the growth of adventitious roots and the biosynthesis of ginsenosides”
Claim · model-written summary
Polysaccharide contents of Panax ginseng adventitious roots decreased with increasing salt strength.
- Taxon
- Panax ginsengPanax ginsengt0
- Treatment
- salt strengthe5
- Outcome
- contents of polysaccharidese5
- Direction
- decreasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adventitious roots culture in Panax ginseng”
e5“The contents of polysaccharides were decreased with the increase of salt strength”
The same questions in other papers
sucrose · growth · Panax ginseng (ginseng)Results agree
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- positive
- positive
- positive
salinity · molecular: metabolites · Panax ginseng (ginseng)Positive and negative results
1 in the opposite direction
- positive
in vitro · growth · Panax ginseng (ginseng)Positive and negative results
9 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.