Abstract
【Objective】 The present study was aimed at investigating the differences of mutant phenotypes resulting from different mutations in base sequence of rice divinyl reductase gene OsDVR(Os03g22780),and evaluating application potential of this gene in rice breeding as a leaf color marker gene.【Method】The yellow-green leaf mutants isolated from Indica rice variety G46B and japonica rice variety Nipponbare via ethyl methanesulfonate mutagenesis were detected using high-performance liquid chromatography(HPLC) to screen the mutants accumulating divinyl chlorophyll.Then genetic analysis,gene mapping and allelism test of the target mutants were performed.Subsequently,DNA sequencing of OsDVR gene in these mutants and alignment of the deduced amino acid sequences of homologous DVR proteins were conducted,and leaf photosynthetic pigments,plant phenotypes and main agronomic traits of the mutants were investigated.【Result】590ys and 525ys,two novel mutants accumulating divinyl chlorophyll,were obtained by screening 53 yellow-green leaf mutants isolated from ethyl methanesulfonate mutagenesis.Their mutant phenotypes were all controlled by a pair of recessive nuclear gene and moreover,the mutant genes were all mapped on the chromosomal region harbouring the 824ys mutant gene reported.Furthermore,allelism test confirmed that the mutant genes of 590ys and 525ys were allelic to that of 824ys.So 590ys,525ys and 824ys mutants were all OsDVR mutants.However,mutational sites of OsDVR mutant gene and their encoded protein product in the three mutants were different,which resulted in extremely significant differences of chlorophyll contents and compositions,plant phenotypes,main agronomic traits and yields per plant among the three mutants.Among them,525ys mutant had slight yellow leaves,and its growth and development,main agronomic traits and yield per plant were affected relatively less,indicating that 525ys mutant gene can basically meet the requirements as a leaf color marker gene introduced into rice male sterile lines.【Conclusion】Different mutations in base sequence of OsDVR gene could result in extremely significant differences of mutant phenotype and yield per plant.The OsDVR mutant gene could be applicable in hybrid rice breeding as a leaf color marker gene.
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Claims 12
Claim · model-written summary
Screening identified two new divinyl chlorophyll accumulating mutants, 590ys and 525ys.
- Taxon
- rice
Oryza sativae1 - Material
- 590ys and 525yse0
- Outcome
- two novel mutants accumulating divinyl chlorophylle0
- Value
- twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“590ys and 525ys,two novel mutants accumulating divinyl chlorophyll,were obtained by screening 53 yellow-green leaf mutants”
e1“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The mutant phenotypes were controlled by one recessive nuclear gene pair.
- Taxon
- riceOryza sativae1
- Gene
- a pair of recessive nuclear genee0
- Outcome
- mutant phenotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Their mutant phenotypes were all controlled by a pair of recessive nuclear gene”
e1“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The 590ys and 525ys genes mapped to the chromosome region containing the 824ys gene.
- Taxon
- riceOryza sativae1
- Gene
- mutant genese0
- Outcome
- mapped on the chromosomal region harbouring the 824ys mutant genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutant genes were all mapped on the chromosomal region harbouring the 824ys mutant gene reported”
e1“rice divinyl reductase gene OsDVR”
Claim · model-written summary
Allelism testing found 590ys and 525ys allelic to 824ys.
- Taxon
- riceOryza sativae1
- Gene
- mutant genes of 590ys and 525yse0
- Outcome
- allelic to that of 824yse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“allelism test confirmed that the mutant genes of 590ys and 525ys were allelic to that of 824ys”
e1“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The three mutants were identified as OsDVR mutants.
- Taxon
- riceOryza sativae1
- Gene
- OsDVRe0
- Outcome
- 590ys,525ys and 824ys mutants were all OsDVR mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“590ys,525ys and 824ys mutants were all OsDVR mutants”
e1“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The three mutants had different OsDVR mutation sites and encoded products.
- Taxon
- riceOryza sativae1
- Gene
- OsDVR mutant genee0
- Outcome
- mutational sites of OsDVR mutant gene and their encoded protein producte0
- Direction
- differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutational sites of OsDVR mutant gene and their encoded protein product in the three mutants were different”
e1“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The three mutations produced extremely significant differences in chlorophyll content and composition.
- Taxon
- riceOryza sativae2
- Gene
- OsDVR mutant genee1
- Outcome
- chlorophyll contents and compositionse0
- Direction
- differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which resulted in extremely significant differences of chlorophyll contents and compositions,plant phenotypes,main agronomic traits and yields per plant among the three mutants”
e1“mutational sites of OsDVR mutant gene and their encoded protein product in the three mutants were different”
e2“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The three mutations produced extremely significant differences in plant phenotypes and agronomic traits.
- Taxon
- riceOryza sativae2
- Gene
- OsDVR mutant genee1
- Outcome
- plant phenotypes,main agronomic traitse0
- Direction
- differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extremely significant differences of chlorophyll contents and compositions,plant phenotypes,main agronomic traits and yields per plant among the three mutants”
e1“mutational sites of OsDVR mutant gene”
e2“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The three mutations produced extremely significant differences in per plant yield.
- Taxon
- riceOryza sativae2
- Gene
- OsDVR mutant genee1
- Outcome
- yields per plante0
- Direction
- differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“extremely significant differences of chlorophyll contents and compositions,plant phenotypes,main agronomic traits and yields per plant among the three mutants”
e1“mutational sites of OsDVR mutant gene”
e2“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The 525ys mutant had slightly yellow leaves.
- Taxon
- riceOryza sativae1
- Material
- 525yse0
- Outcome
- slight yellow leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“525ys mutant had slight yellow leaves”
e1“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The 525ys mutant had relatively little impairment of growth, agronomic traits and yield.
- Taxon
- riceOryza sativae1
- Material
- 525yse0
- Outcome
- growth and development,main agronomic traits and yield per plante0
- Direction
- affected relatively lesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“525ys mutant had slight yellow leaves,and its growth and development,main agronomic traits and yield per plant were affected relatively less”
e1“rice divinyl reductase gene OsDVR”
Claim · model-written summary
The authors judged 525ys suitable as a leaf color marker in rice male sterile lines.
- Taxon
- riceOryza sativae0
- Gene
- 525ys mutant genee0
- Outcome
- leaf color marker gene introduced into rice male sterile linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“525ys mutant gene can basically meet the requirements as a leaf color marker gene introduced into rice male sterile lines”