Abstract
The risk assessment of Bar gene transfer from B and D genomes of transformed wheat lines to jointed goatgrass was done by investigating the seed set of hybrids of crosses and back crossess.Transformed Bobwhite 31 line with Bar gene in B genome,71 line with Bar gene in D genome and jointed goatgrass served as the parents.Three ways of pollination,including non emasculation and spontaneous pollination,emasculation and spontaneous pollination,and emasculation and artificial pollination were adopted in order to verify the effects to bar gene transfer under the different pollination conditions.By testing the ratio of resistant plants in backcross population the safety assessment to Bar gene locating on B genome and on D genome was done.The results showed that the seed set was very poor using the way of non emasculation and spontaneous pollination,actually no seed was obtained in this experiment,however,the hybrids were available under the condition of artificial pollination.As to Bar gene transfer there was significant difference between the gene loci in B genome and in D genome.The former was more difficult to be transferred from transformed wheat to jointed goatgrass than the later,because wheat consists of AABBDD genomes and jointed goatgrass is CCDD genomes,which share a common D genome,so the chromosomes of D genome will not become univalent and be lost in forming gamete.In this experiment,a higher ratio of resistant plants was found in population of (71 line×jointed goatgrass)×jointed goatgrass,and no resistant plants existed in the population of (31 line×jointed goatgrass)×jointed goatgrass.Therefore the transformed wheat with Bar gene in B or A genome can be used to control grass in order to mitigate the risk of gene transfer from transformed wheat to jointed goatgrass.
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Claims 6
Claim · model-written summary
Non-emasculated spontaneous pollination produced no hybrid seed.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- non emasculation and spontaneous pollinatione0
- Outcome
- seed sete0
- Direction
- very poore0
- Comparator
- artificial pollinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed set was very poor using the way of non emasculation and spontaneous pollination,actually no seed was obtained in this experiment,however,the hybrids were available under the condition of artificial pollination”
t0“wheat”
Claim · model-written summary
Artificial pollination yielded hybrids.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- artificial pollinatione0
- Outcome
- hybrids were availablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the hybrids were available under the condition of artificial pollination”
t0“wheat”
Claim · model-written summary
Bar gene transfer differed significantly by genome location, with B harder to transfer than D.
- Taxon
- wheatTriticum aestivume0
- Gene
- Bar genee1
- Outcome
- transferred from transformed wheat to jointed goatgrasse0
- Direction
- more difficulte0
- Comparator
- D genomee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was significant difference between the gene loci in B genome and in D genome.The former was more difficult to be transferred from transformed wheat to jointed goatgrass than the later”
e1“transformed wheat with Bar gene in B or A genome can be used to control grass in order to mitigate the risk of gene transfer from transformed wheat to jointed goatgrass”
Claim · model-written summary
The D genome cross yielded a higher ratio of resistant plants.
- Taxon
- wheatTriticum aestivumt0
- Material
- 71 linee0
- Interactor
- jointed goatgrasse0weed
- Outcome
- ratio of resistant plantse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a higher ratio of resistant plants was found in population of (71 line×jointed goatgrass)×jointed goatgrass”
t0“wheat”
Claim · model-written summary
The B genome cross yielded no resistant plants.
- Taxon
- wheatTriticum aestivumt0
- Material
- 31 linee0
- Interactor
- jointed goatgrasse0weed
- Outcome
- resistant plantse0
- Direction
- noe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no resistant plants existed in the population of (31 line×jointed goatgrass)×jointed goatgrass”
t0“wheat”
Claim · model-written summary
The authors recommend transformed wheat carrying Bar in the B or A genome to control grass while mitigating gene transfer.
- Taxon
- wheatTriticum aestivume0
- Interactor
- jointed goatgrasse0weed
- Gene
- Bar genee0
- Outcome
- control grass in order to mitigate the risk of gene transfere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transformed wheat with Bar gene in B or A genome can be used to control grass in order to mitigate the risk of gene transfer from transformed wheat to jointed goatgrass”
The same questions in other papers
pollination · yield · Triticum aestivum (wheat)Positive and negative results
3 papers in the same direction as this paper · 8 in the opposite direction
- positive
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.