Paper · 2009 · Acta Agriculturae Boreali-Sinica

The Physiological,Biochemical and Chloroplast Proteomic Changes of Wheat Under the Induction of Copper

Baoli Fan

7
claims
13
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

The aim of studying the effects of heavy metal copper which is one of the heavy metal pollutants on the physiological,biochemical characteristics and chloroplast proteome of the wheat seedlings is to explore its influence on the growth of wheat seedlings and chloroplast proteome.Treat the wheat seedlings with CuCl2 for 5 days,measure the length of seedlings and determine the content of chlorophyll and soluble protein,the results indicated that with the concentration of Cu2+increased,the height of wheat seedlings significantly decreased,the height of seedlings under each concentration was significantly lower than the control,54.47% decreased with the concentration of 5 mmol/L in contrast to the control;the chlorophyll content showed the trend of decrease-increase-decrease,but all less than the control;the soluble protein content decreased 0.68-1.23 mg/g in contrast to the control with no significant distinction.Analyze the change of chloroplast proteome by two-dimensional electrophoresis(2-DE) with the Cu2+ concentration of 1 mmol/L,the result showed that 17 protein spots significantly changed,seven spots disappeared,one spot appeared,and nine spots down-expressed.

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Claims 7

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Increasing copper concentration significantly reduced wheat seedling height; at 5 mmol/L it fell 54.47% versus control.

Treatment
Cu2+e0
Outcome
height of wheat seedlingse0
Direction
decreasede0
Value
54.47%e0
Qualifier
5 mmol/Le0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“with the concentration of Cu2+increased,the height of wheat seedlings significantly decreased,the height of seedlings under each concentration was significantly lower than the control,54.47% decreased with the concentration of 5 mmol/L in contrast to the control”

✓ located verbatim in the source · abstract, characters 448–710 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Chlorophyll content fluctuated across copper concentrations but remained below control.

Treatment
Coppert0
Outcome
chlorophyll contente0
Direction
less thane0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the chlorophyll content showed the trend of decrease-increase-decrease,but all less than the control”

✓ located verbatim in the source · abstract, characters 711–811 · show in text ↑
t0

“Wheat Under the Induction of Copper”

✓ located verbatim in the source · title, characters 67–102 · show in text ↑
Intervention effectobservedoriginal resultnegativenot significant (as stated)measured

Claim · model-written summary

Soluble protein content declined by 0.68-1.23 mg/g versus control without a significant distinction.

Treatment
Coppert0
Outcome
soluble protein contente0
Direction
decreasede0
Value
0.68-1.23 mg/ge0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the soluble protein content decreased 0.68-1.23 mg/g in contrast to the control with no significant distinction”

✓ located verbatim in the source · abstract, characters 812–923 · show in text ↑
t0

“Wheat Under the Induction of Copper”

✓ located verbatim in the source · title, characters 67–102 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

At 1 mmol/L Cu2+, 17 chloroplast protein spots changed significantly.

Treatment
Cu2+ concentration of 1 mmol/Le0
Outcome
protein spotse0
Value
17 protein spotse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“with the Cu2+ concentration of 1 mmol/L,the result showed that 17 protein spots significantly changed”

✓ located verbatim in the source · abstract, characters 1008–1109 · show in text ↑
t0

“Wheat Under the Induction of Copper”

✓ located verbatim in the source · title, characters 67–102 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Seven chloroplast protein spots disappeared after copper exposure.

Treatment
Coppert0
Outcome
spots disappearede0
Value
seven spotse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“seven spots disappeared”

✓ located verbatim in the source · abstract, characters 1110–1133 · show in text ↑
t0

“Wheat Under the Induction of Copper”

✓ located verbatim in the source · title, characters 67–102 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

One chloroplast protein spot appeared after copper exposure.

Treatment
Coppert0
Outcome
spot appearede0
Value
one spote0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“one spot appeared”

✓ located verbatim in the source · abstract, characters 1134–1151 · show in text ↑
t0

“Wheat Under the Induction of Copper”

✓ located verbatim in the source · title, characters 67–102 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Nine chloroplast protein spots were down expressed after copper exposure.

Treatment
Coppert0
Outcome
spotse0
Direction
down-expressede0
Value
nine spotse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“nine spots down-expressed”

✓ located verbatim in the source · abstract, characters 1156–1181 · show in text ↑
t0

“Wheat Under the Induction of Copper”

✓ located verbatim in the source · title, characters 67–102 · show in text ↑

copper atom · physiology: osmolytes · Triticum aestivum (wheat)Results differ

7 papers: 1 positive, 6 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

copper atom · physiology: pigments · Triticum aestivum (wheat)Results differ

16 papers: 9 positive, 7 negative or no effect

6 papers in the same direction as this paper · 9 in the opposite direction

  1. positive
  2. positive
  3. positive

12 papers more in this group

See all 16 papers →

copper(2+) · growth · Triticum aestivum (wheat)Positive and negative results

8 papers: 4 positive, 4 negative or no effect

3 papers in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.