Abstract
Different explants of alfalfa(Medicago Sativa L.)SANDITI seedling were cultured on the media of different phytohormone combinations.The results indicated that MS+24-D 2.0 mg/L +6-BA 0.5 mg/L medium was the best to induce callus with the highest induction frequency from hypocotyl.Reducing the concentrations of auxin and raising the concentrations of kinin was benefit for the reducing of browing rate.The calli from hypocotyls were cultured in liquid medium SH+24-D 1.0 mg/L+6-BA 0.5 mg/L+2 000 mg/L,somatic embryos appesred.The higher regeneration rate can be get from MS medium with 6-BA 0.5 mg/L,NAA 0.1 mg/L and GA3 1.0 mg/L.
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Claims 4
Claim · model-written summary
MS with 24-D and 6-BA gave the highest callus induction frequency from alfalfa hypocotyl.
- Taxon
- Medicago Sativa L.t0
- Organ / stage
- hypocotyle0
- Treatment
- MS+24-D 2.0 mg/L +6-BA 0.5 mg/L mediume0
- Outcome
- induction frequencye0
- Direction
- higheste0
Not reportedComparator
Verbatim
e0“MS+24-D 2.0 mg/L +6-BA 0.5 mg/L medium was the best to induce callus with the highest induction frequency from hypocotyl”
t0“Medicago Sativa L.”
Claim · model-written summary
Lower auxin and higher kinin concentrations reduced browning.
- Taxon
- Medicago Sativa L.t0
- Treatment
- Reducing the concentrations of auxin and raising the concentrations of kinine0
- Outcome
- browing ratee0
- Direction
- reducinge0
Not reportedComparator
Verbatim
e0“Reducing the concentrations of auxin and raising the concentrations of kinin was benefit for the reducing of browing rate.”
t0“Medicago Sativa L.”
Claim · model-written summary
Hypocotyl calli formed somatic embryos in the specified SH liquid medium.
Verbatim
e0“The calli from hypocotyls were cultured in liquid medium SH+24-D 1.0 mg/L+6-BA 0.5 mg/L+2 000 mg/L,somatic embryos appesred.”
t0“Medicago Sativa L.”
Claim · model-written summary
MS medium with 6-BA, NAA and GA3 gave a higher regeneration rate.
- Taxon
- Medicago Sativa L.t0
- Treatment
- MS medium with 6-BA 0.5 mg/L,NAA 0.1 mg/L and GA3 1.0 mg/Le0
- Outcome
- regeneration ratee0
- Direction
- highere0
Not reportedComparator
Verbatim
e0“The higher regeneration rate can be get from MS medium with 6-BA 0.5 mg/L,NAA 0.1 mg/L and GA3 1.0 mg/L.”
t0“Medicago Sativa L.”
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