Abstract
In order to improve the anther culture efficiency of alfalfa,effects of glutamine,proline and exogenous hormones on anther culture were studied.Alfalfa anthers were used as explants with pollen in the middle development stage and /or in the side development stage of single nucleus.The results indicate that culture medium supplemented with glutamine or proline was benefit for the callus induction,proliferation and green plantlets differentiation and concentration of glutamine at 700 mg/L or proline at 600 mg/L had better effect.Callus proliferation medium with 2,4-D at 2 mg/L and 6-BA at 0.5 mg/L in combination obviously enhanced the callus growth and green plantlets formation than singly used.Green plantlets differentiation rate was better with addition of NAA at 0.5 mg/L and KT at 2 mg/L to callus differentiation medium.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher.
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Glutamine supplementation benefited callus induction, proliferation and green plantlet differentiation.
- Treatment
- glutaminee0
- Outcome
- callus induction,proliferation and green plantlets differentiatione0
- Direction
- benefite0
Not reportedTaxonComparator
Verbatim
e0“culture medium supplemented with glutamine or proline was benefit for the callus induction,proliferation and green plantlets differentiation”
Claim · model-written summary
Proline supplementation benefited callus induction, proliferation and green plantlet differentiation.
- Treatment
- prolinee0
- Outcome
- callus induction,proliferation and green plantlets differentiatione0
- Direction
- benefite0
Not reportedTaxonComparator
Verbatim
e0“culture medium supplemented with glutamine or proline was benefit for the callus induction,proliferation and green plantlets differentiation”
Claim · model-written summary
Glutamine at 700 mg/L had a better effect on culture outcomes.
- Taxon
- Alfalfa
Medicago sativat0 - Treatment
- glutamine at 700 mg/Le0
- Outcome
- better effecte0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“concentration of glutamine at 700 mg/L or proline at 600 mg/L had better effect”
t0“Alfalfa”
Claim · model-written summary
Proline at 600 mg/L had a better effect on culture outcomes.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- proline at 600 mg/Le0
- Outcome
- better effecte0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“concentration of glutamine at 700 mg/L or proline at 600 mg/L had better effect”
t0“Alfalfa”
Claim · model-written summary
Combined 2,4-D and 6-BA enhanced callus growth relative to individual use.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- 2,4-D at 2 mg/L and 6-BA at 0.5 mg/L in combinatione0
- Outcome
- callus growthe0
- Direction
- enhancede0
- Comparator
- singly usede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus proliferation medium with 2,4-D at 2 mg/L and 6-BA at 0.5 mg/L in combination obviously enhanced the callus growth and green plantlets formation than singly used.”
t0“Alfalfa”
Claim · model-written summary
Combined 2,4-D and 6-BA enhanced green plantlet formation relative to individual use.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- 2,4-D at 2 mg/L and 6-BA at 0.5 mg/L in combinatione0
- Outcome
- green plantlets formatione0
- Direction
- enhancede0
- Comparator
- singly usede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus proliferation medium with 2,4-D at 2 mg/L and 6-BA at 0.5 mg/L in combination obviously enhanced the callus growth and green plantlets formation than singly used.”
t0“Alfalfa”
Claim · model-written summary
NAA and KT supplementation improved green plantlet differentiation rate.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- addition of NAA at 0.5 mg/L and KT at 2 mg/Le0
- Outcome
- Green plantlets differentiation ratee0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Green plantlets differentiation rate was better with addition of NAA at 0.5 mg/L and KT at 2 mg/L to callus differentiation medium.”
t0“Alfalfa”
The same questions in other papers
proline · growth · Medicago sativa (alfalfa)Results differ
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
N-benzyladenine · growth · Medicago sativa (alfalfa)Positive and negative results
9 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
7 papers more in this group
See all 11 papers →2,4-D · growth · Medicago sativa (alfalfa)Positive and negative results
15 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.