Abstract
The cold tolerance of rice (Oryza sativa L.) seedlings could be markedly improved by mild oxidative pretreatment (10 mumol/L methyl viologen (MV). The enhancement of chilling resistance induced by MV pretreatment could be inhibited by Ca(2+)-chelate ethyleneglycol-bis-(beta-aminoethylether) N,N-tetraacetic acid (EGTA, 10 mmol/L) and the calmodulin inhibitor chlorpromazine (CPZ, 0.5 mmol/L). The Ca(2+)-ATPase activity of root plasmolemma and tonoplast membrane as well as the Fe(CN)6(3-) reduction of plasmolemma were also enhanced by MV pretreatment. However, the above MV pretreatment effect could be inhibited by EGTA and CPZ, respectively. In vitro, Ca(2+)-ATPase activities in the two types of membrane vesicles were reduced by reactive oxygen species (H2O2, O2-., .OH). Results indicated that the enhancement of chilling resistance in rice seedlings with MV pretreatment was likely related to the effective activation of Ca(2+)-ATPase induced by calcium messenger of CaM, and the improved cold tolerance of the activities might be associated with the augmentation of cellular membrane protective ability caused by the MV pretreatment.
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Claims 9
Claim · model-written summary
Mild methyl viologen pretreatment improved rice seedling cold tolerance.
- Taxon
- rice
Oryza sativae0 - Treatment
- 10 mumol/L methyl viologen (MV)e0
- Outcome
- cold tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold tolerance of rice (Oryza sativa L.) seedlings could be markedly improved by mild oxidative pretreatment (10 mumol/L methyl viologen (MV)”
Claim · model-written summary
EGTA inhibited the increased chilling resistance induced by methyl viologen.
Not reportedTaxonComparator
Verbatim
e0“enhancement of chilling resistance induced by MV pretreatment could be inhibited by Ca(2+)-chelate ethyleneglycol-bis-(beta-aminoethylether) N,N-tetraacetic acid (EGTA, 10 mmol/L)”
Claim · model-written summary
Chlorpromazine inhibited the increased chilling resistance induced by methyl viologen.
- Treatment
- chlorpromazine (CPZ, 0.5 mmol/L)e0
- Outcome
- enhancement of chilling resistancee0
- Direction
- inhibitede0
Not reportedTaxonComparator
Verbatim
e0“enhancement of chilling resistance induced by MV pretreatment could be inhibited by Ca(2+)-chelate ethyleneglycol-bis-(beta-aminoethylether) N,N-tetraacetic acid (EGTA, 10 mmol/L) and the calmodulin inhibitor chlorpromazine (CPZ, 0.5 mmol/L)”
Claim · model-written summary
MV increased Ca(2+)-ATPase activity in root plasmolemma and tonoplast membranes.
- Treatment
- MV pretreatmente0
- Gene
- Ca(2+)-ATPasee0
- Outcome
- Ca(2+)-ATPase activity of root plasmolemma and tonoplast membranee0
- Direction
- enhancede0
Not reportedTaxonComparator
Verbatim
e0“Ca(2+)-ATPase activity of root plasmolemma and tonoplast membrane as well as the Fe(CN)6(3-) reduction of plasmolemma were also enhanced by MV pretreatment”
Claim · model-written summary
MV increased plasmolemma ferricyanide reduction.
Not reportedTaxonComparator
Verbatim
e0“Fe(CN)6(3-) reduction of plasmolemma were also enhanced by MV pretreatment”
Claim · model-written summary
EGTA inhibited the MV pretreatment effect on membrane activity.
Not reportedTaxonComparator
Verbatim
e0“the above MV pretreatment effect could be inhibited by EGTA and CPZ, respectively”
Claim · model-written summary
CPZ inhibited the MV pretreatment effect on membrane activity.
Not reportedTaxonComparator
Verbatim
e0“the above MV pretreatment effect could be inhibited by EGTA and CPZ, respectively”
Claim · model-written summary
Reactive oxygen species reduced Ca(2+)-ATPase activity in membrane vesicles in vitro.
- Treatment
- reactive oxygen species (H2O2, O2-., .OH)e0
- Outcome
- Ca(2+)-ATPase activitiese0
- Direction
- reducede0
Not reportedTaxonComparator
Verbatim
e0“In vitro, Ca(2+)-ATPase activities in the two types of membrane vesicles were reduced by reactive oxygen species (H2O2, O2-., .OH)”
Claim · model-written summary
The authors proposed calcium messenger activation of Ca(2+)-ATPase as related to MV-induced chilling resistance.
- Taxon
- riceOryza sativae0
- Gene
- Ca(2+)-ATPasee0
- Outcome
- enhancement of chilling resistancee0
- Qualifier
- likely related to the effective activatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhancement of chilling resistance in rice seedlings with MV pretreatment was likely related to the effective activation of Ca(2+)-ATPase induced by calcium messenger of CaM”
The same questions in other papers
reactive oxygen species · nutrient: content · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- positive
hydrogen peroxide · nutrient: content · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.