Abstract
Crosses were attempted between accessions belonging to G. hirsutum and G. arboreum to develop interspecific hybrids through in-ovulo embryo culture. A mixture of growth regulators (GA3 @ 50 ppm + NAA @ 100 ppm) was applied at the base of pedicel of crossed buds for three successive days starting from day of pollination. The ovules were excised and cultured three days after pollination on various tissue culture media. Of the 26,300 ovules cultured, 33 germinated with germination percentage of 0.12. Germination was observed in seven crosses namely, LH 2108 x LD 327 (0.57%), F 2164 x LD 949 (0.33%), F 2164 x FDK 124 (0.30%), PIL 43 x LD 949 (0.083%), LH 2108 x LD 949 (0.066%), LH 2108 x FDK 124 (0.044%) and LH 1556 x LD 491 (0.083%). Two media namely MS4 (MS + Gamborg's B5 vitamins + 1.9 g/l KNO3) and MS5 (MS + 1.0 mg/l IAA + 0.2 mg/l Kin) were found to promote more embryo germination. Hybridity of the interspecific hybrid between LH 2108 x LD 327 was confirmed using microsatellite markers.
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Claims 11
Claim · model-written summary
Thirty-three of 26,300 cultured ovules germinated, for 0.12% germination.
- Taxon
- Gossypium hirsutum and Gossypium arboreum
Gossypium hirsutum
Gossypium arboreumt0 - Organ / stage
- ovulese0
- Outcome
- germination percentagee0
- Value
- 0.12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Of the 26,300 ovules cultured, 33 germinated with germination percentage of 0.12.”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
LH 2108 x LD 327 ovules germinated at 0.57%.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Material
- LH 2108 x LD 327e0
- Outcome
- Germinatione1
- Value
- 0.57%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LH 2108 x LD 327 (0.57%)”
e1“Germination was observed in seven crosses namely”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
F 2164 x LD 949 ovules germinated at 0.33%.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Material
- F 2164 x LD 949e0
- Outcome
- Germinatione1
- Value
- 0.33%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F 2164 x LD 949 (0.33%)”
e1“Germination was observed in seven crosses namely”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
F 2164 x FDK 124 ovules germinated at 0.30%.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Material
- F 2164 x FDK 124e0
- Outcome
- Germinatione1
- Value
- 0.30%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“F 2164 x FDK 124 (0.30%)”
e1“Germination was observed in seven crosses namely”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
PIL 43 x LD 949 ovules germinated at 0.083%.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Material
- PIL 43 x LD 949e0
- Outcome
- Germinatione1
- Value
- 0.083%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PIL 43 x LD 949 (0.083%)”
e1“Germination was observed in seven crosses namely”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
LH 2108 x LD 949 ovules germinated at 0.066%.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Material
- LH 2108 x LD 949e0
- Outcome
- Germinatione1
- Value
- 0.066%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LH 2108 x LD 949 (0.066%)”
e1“Germination was observed in seven crosses namely”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
LH 2108 x FDK 124 ovules germinated at 0.044%.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Material
- LH 2108 x FDK 124e0
- Outcome
- Germinatione1
- Value
- 0.044%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LH 2108 x FDK 124 (0.044%)”
e1“Germination was observed in seven crosses namely”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
LH 1556 x LD 491 ovules germinated at 0.083%.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Material
- LH 1556 x LD 491e0
- Outcome
- Germinatione1
- Value
- 0.083%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LH 1556 x LD 491 (0.083%)”
e1“Germination was observed in seven crosses namely”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
MS4 medium promoted more embryo germination.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Treatment
- MS4 (MS + Gamborg's B5 vitamins + 1.9 g/l KNO3)e0
- Outcome
- embryo germinatione0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two media namely MS4 (MS + Gamborg's B5 vitamins + 1.9 g/l KNO3) and MS5 (MS + 1.0 mg/l IAA + 0.2 mg/l Kin) were found to promote more embryo germination.”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
MS5 medium promoted more embryo germination.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Treatment
- MS5 (MS + 1.0 mg/l IAA + 0.2 mg/l Kin)e0
- Outcome
- embryo germinatione0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two media namely MS4 (MS + Gamborg's B5 vitamins + 1.9 g/l KNO3) and MS5 (MS + 1.0 mg/l IAA + 0.2 mg/l Kin) were found to promote more embryo germination.”
t0“Gossypium hirsutum and Gossypium arboreum”
Claim · model-written summary
Microsatellite markers confirmed hybridity of LH 2108 x LD 327.
- Taxon
- Gossypium hirsutum and Gossypium arboreumGossypium hirsutumGossypium arboreumt0
- Material
- LH 2108 x LD 327e0
- Outcome
- Hybriditye0
- Comparator
- microsatellite markerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hybridity of the interspecific hybrid between LH 2108 x LD 327 was confirmed using microsatellite markers.”
t0“Gossypium hirsutum and Gossypium arboreum”
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