Abstract
Somatic embryogenesis and plant regeneration are fundamental to tissue culture biotechnology in cotton (Gossypium hirsutum L.) cv. Coker 312. Callus proliferation was considered best on MS1a (2.0 mg/L NAA; 0.1 mg/L ZT; 0.1 mg/L KT) when 6 weeks old callus was cultured from MS1b (0.1 mg/L 2, 4-D; 0.5 mg/L KT) medium, there is no need to select embryogenic calli for somatic embryogenesis, as all of them were converted to somatic embryos. NH4NO3 play an important role in differentiation of callus into somatic embryos but is lethal for embryos just after two weeks. However, KNO3 is less efficient for somatic embryo induction but is best for embryo maturation. By this procedure 56.51% cotyledenary embryos were developed within 5 weeks. Of that, 82.05% cotyledenary embryos were developed not only into normal plantlets, but rooted simultaneously when cultured on MS (with 0.05 mg/L GA3) medium. A complete plant of Cocker-312 could be regenerated through somatic embryogenesis within 4 to 5 months.
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Claims 9
Claim · model-written summary
Callus proliferation of cotton was best on MS1a medium when 6-week-old callus was cultured from MS1b medium.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- MS1a (2.0 mg/L NAA; 0.1 mg/L ZT; 0.1 mg/L KT)e0
- Outcome
- Callus proliferatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus proliferation was considered best on MS1a (2.0 mg/L NAA; 0.1 mg/L ZT; 0.1 mg/L KT) when 6 weeks old callus was cultured from MS1b (0.1 mg/L 2, 4-D; 0.5 mg/L KT) medium”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
There was no need to select embryogenic calli for somatic embryogenesis in cotton because all calli converted to somatic embryos.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- all of them were converted to somatic embryose0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there is no need to select embryogenic calli for somatic embryogenesis, as all of them were converted to somatic embryos”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
NH4NO3 plays an important role in differentiation of cotton callus into somatic embryos.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- NH4NO3e0
- Outcome
- differentiation of callus into somatic embryose0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NH4NO3 play an important role in differentiation of callus into somatic embryos but is lethal for embryos just after two weeks”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
NH4NO3 is lethal for cotton somatic embryos just after two weeks.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- NH4NO3e0
- Outcome
- lethal for embryose0
- Qualifier
- just after two weekse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NH4NO3 play an important role in differentiation of callus into somatic embryos but is lethal for embryos just after two weeks”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
KNO3 was less efficient than other treatments for somatic embryo induction in cotton.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- KNO3e0
- Outcome
- somatic embryo inductione0
- Direction
- less efficiente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“KNO3 is less efficient for somatic embryo induction but is best for embryo maturation”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
KNO3 was best for somatic embryo maturation in cotton.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- KNO3e0
- Outcome
- embryo maturatione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“KNO3 is less efficient for somatic embryo induction but is best for embryo maturation”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
56.51% of cotton somatic embryos developed to the cotyledonary stage within 5 weeks.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- cotyledenary embryose0
- Value
- 56.51%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“By this procedure 56.51% cotyledenary embryos were developed within 5 weeks”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
82.05% of cotyledonary embryos developed into normal plantlets that rooted simultaneously on MS medium with 0.05 mg/L GA3.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- normal plantletse0
- Value
- 82.05%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Of that, 82.05% cotyledenary embryos were developed not only into normal plantlets, but rooted simultaneously when cultured on MS (with 0.05 mg/L GA3) medium”
t0“cotton (Gossypium hirsutum L.)”
Claim · model-written summary
A complete plant of cotton Coker-312 could be regenerated through somatic embryogenesis within 4 to 5 months.
- Taxon
- cottonGossypium hirsutumt0
- Material
- Cocker-312e0
- Outcome
- complete plante0
- Qualifier
- within 4 to 5 monthse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A complete plant of Cocker-312 could be regenerated through somatic embryogenesis within 4 to 5 months”
t0“cotton (Gossypium hirsutum L.)”
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