Abstract
The effect of the concentration and proportion of growth regulators in an initial medium on Narcissus L. ‘Carlton’ morphogenesis in cultures of Pc stage flower bud explants was investigated. Ovary and flower stem explants were cultured on solid Murashige and Skoog (MS) media with cytokinin – 6-Benzyladenine (BA) or Zeatin (ZEA) (0.5–50 μM) – in combination with auxin – 4-Amino-3,5,6-trichloropicolinic acid (Picloram), 2,4-Dichlorophenoxyacetic acid (2,4-D) or α-Naphthaleneacetic acid (NAA) (1–50 μM). The ratios of cytokinin and auxin in the initial medium determined the morphogenetic pathways and the level of cytokinin proved to be a critical factor. Somatic embryogenesis was achieved for a cytokinin and auxin ratio of 1:1–10, bulb organogenesis – for a 1:0.4–1 ratio and rhizogenesis – for a 1:20–100 ratio. The minimum concentration of cytokinin in the initial medium for somatic embryo and bulb formation was 5 μM. A lower level of cytokinin (0.5 μM) resulted in rhizogenesis. Considering the effect of auxin, Picloram stimulated the initiation of somatic embryos and the 2,4-D enhanced embryo maturation and conversion.
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Claims 8
Claim · model-written summary
The cytokinin and auxin ratio in the initial medium determined the morphogenetic pathways of Narcissus explants, and the cytokinin level was a critical factor.
NarcissusNot reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The ratios of cytokinin and auxin in the initial medium determined the morphogenetic pathways and the level of cytokinin proved to be a critical factor”
t0“Narcissus L.”
Claim · model-written summary
Somatic embryogenesis was achieved at a cytokinin to auxin ratio of 1:1-10.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Somatic embryogenesis was achieved for a cytokinin and auxin ratio of 1:1–10”
t0“Narcissus L.”
Claim · model-written summary
Bulb organogenesis was achieved at a cytokinin to auxin ratio of 1:0.4-1.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“bulb organogenesis – for a 1:0.4–1 ratio”
t0“Narcissus L.”
Claim · model-written summary
Rhizogenesis was achieved at a cytokinin to auxin ratio of 1:20-100.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“rhizogenesis – for a 1:20–100 ratio”
t0“Narcissus L.”
Claim · model-written summary
The minimum cytokinin concentration in the initial medium for somatic embryo and bulb formation was 5 uM.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The minimum concentration of cytokinin in the initial medium for somatic embryo and bulb formation was 5 μM”
t0“Narcissus L.”
Claim · model-written summary
A lower cytokinin level (0.5 uM) resulted in rhizogenesis.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A lower level of cytokinin (0.5 μM) resulted in rhizogenesis”
t0“Narcissus L.”
Claim · model-written summary
Picloram stimulated the initiation of somatic embryos.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Picloram stimulated the initiation of somatic embryos”
t0“Narcissus L.”
Claim · model-written summary
2,4-D enhanced embryo maturation and conversion.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the 2,4-D enhanced embryo maturation and conversion”
t0“Narcissus L.”
The same questions in other papers
class · growth · NarcissusResults agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.