Abstract
Soybean mosaic virus (SMV) causes one of the most destructive viral diseases in soybean (Glycine max). The soybean cultivar Dabaima carries the Rsc4 gene for SMV resistance. The genomic region containing Rsc4 was previously localised within a 100-kb region on chromosome 14. The corresponding region contains three complete nucleotide-binding site (NB) and leucine-rich repeat (LRR) type genes and one incomplete gene that is likely non-functional. Quantitative real-time polymerase chain reaction analysis revealed that three candidate genes encoding NB-LRR proteins were differentially expressed in resistant and susceptible lines when the plants were inoculated with SMV strain SC4. To test the involvement of the three candidate genes in Rsc4 mediated resistance, the three genes were silenced using a Bean pod mottle virus (BPMV)-based vector construct. Silencing of three candidate genes attenuated the Rsc4-mediated resistance and induced SMV symptoms in Dabaima plants. Moreover, Rsc4 candidate genes were 78% downregulated when compared with the empty BPMV vector-treated plants. From these results, we concluded that at least one of the three candidate genes encoding NB-LRR proteins is required for Rsc4 resistance to SMV.
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Claims 4
Claim · model-written summary
Three candidate genes encoding NB-LRR proteins were differentially expressed in resistant and susceptible soybean lines inoculated with SMV strain SC4.
- Taxon
- soybean
Glycine maxe0 - Material
- resistant and susceptible linese1
- Interactor
- SMV strain SC4e1pathogen
- Gene
- NB-LRR proteinse1
- Outcome
- three candidate genes encoding NB-LRR proteinse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean mosaic virus (SMV) causes one of the most destructive viral diseases in soybean (Glycine max)”
e1“Quantitative real-time polymerase chain reaction analysis revealed that three candidate genes encoding NB-LRR proteins were differentially expressed in resistant and susceptible lines when the plants were inoculated with SMV strain SC4”
Claim · model-written summary
Silencing of three candidate genes attenuated Rsc4-mediated resistance and induced SMV symptoms in Dabaima soybean plants.
- Taxon
- soybeanGlycine maxe0
- Material
- Dabaimae1
- Treatment
- Silencing of three candidate genese1
- Interactor
- SMVe1pathogen
- Gene
- Rsc4e1
- Outcome
- Rsc4-mediated resistancee1
- Direction
- attenuatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean mosaic virus (SMV) causes one of the most destructive viral diseases in soybean (Glycine max)”
e1“Silencing of three candidate genes attenuated the Rsc4-mediated resistance and induced SMV symptoms in Dabaima plants”
Claim · model-written summary
Silencing with a BPMV-based vector downregulated Rsc4 candidate genes by 78% compared with the empty vector.
- Taxon
- soybeanGlycine maxe0
- Treatment
- silenced using a Bean pod mottle virus (BPMV)-based vector constructe1
- Outcome
- Rsc4 candidate genese2
- Direction
- downregulatede2
- Value
- 78%e2
- Comparator
- empty BPMV vector-treated plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean mosaic virus (SMV) causes one of the most destructive viral diseases in soybean (Glycine max)”
e1“the three genes were silenced using a Bean pod mottle virus (BPMV)-based vector construct”
e2“Moreover, Rsc4 candidate genes were 78% downregulated when compared with the empty BPMV vector-treated plants”
Claim · model-written summary
At least one of the three NB-LRR candidate genes is required for Rsc4 resistance to SMV in soybean.
- Taxon
- soybeanGlycine maxe0
- Interactor
- SMVe1pathogen
- Gene
- NB-LRR proteinse1
- Outcome
- Rsc4 resistance to SMVe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean mosaic virus (SMV) causes one of the most destructive viral diseases in soybean (Glycine max)”
e1“we concluded that at least one of the three candidate genes encoding NB-LRR proteins is required for Rsc4 resistance to SMV”
The same questions in other papers
loss of function · disease · Glycine max (soybean)Positive and negative results
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See all 37 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.