Abstract
Developing somatic and zygotic embryos of alfalfa (Medicago sativa L.) exhibited endogenous protein kinase activity and protein acceptors of phosphate groups using both cell-free translational extracts and oligo(dT)-cellulose-column-purified mRNPs. The cell-free-translation extracts from pre-cotyledonary-stage somatic embryos had approximately 50- and 100-fold more protein kinase activity than cotyledonary-stage somatic and zygotic embryos. Several polypeptides were phosphorylated; some of them were unique to the early stage and some to the late-stage developing embryos. A 65 kDa protein was phosphorylated heavily in pre-cotyledonary-stage somatic embryos. This phosphorylated protein was comprised of three main components, two of which were phosphorylated heavily. Heat-shock treated-embryos lost their exitant kinase activity and at the same time another form of protein kinase activity was activated which phosphorylated a novel 28 kDa protein. Endogenous protein kinase activity was also observed within the mRNPs of polysomal and non-polysomal fractions of developing embryos, and this phosphorylated only 65, 43 and 30 kDa proteins within these fractions. A 30 kDa protein from the pre-cotyledonary-stage somatic embryos showed a higher affinity for accepting phosphate groups than the proteins from cotyledonary-stage somatic or zygotic embryos. The activity of protein kinase was largely c-AMP-independent, but was dependent on Ca2+, phospholipid and phorbol ester. The enzyme belongs to the protein kinase-C family; the 65 kDa protein cross-reacts with antibodies made against protein kinase-C (alpha- and beta-isoforms) and it may be an autophosphorylated protein.
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Claims 15
Claim · model-written summary
Developing alfalfa embryos exhibited endogenous kinase activity and phosphate acceptors.
- Taxon
- alfalfa
Medicago sativae0 - Organ / stage
- somatic and zygotic embryose0
- Outcome
- endogenous protein kinase activity and protein acceptors of phosphate groupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Developing somatic and zygotic embryos of alfalfa (Medicago sativa L.) exhibited endogenous protein kinase activity and protein acceptors of phosphate groups using both cell-free translational extracts and oligo(dT)-cellulose-column-purified mRNPs”
Claim · model-written summary
Precotyledonary somatic extracts had roughly 50-fold more kinase activity than cotyledonary somatic extracts.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- pre-cotyledonary-stage somatic embryose0
- Outcome
- protein kinase activitye0
- Direction
- moree0
- Value
- 50-e0
- Comparator
- cotyledonary-stage somatice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cell-free-translation extracts from pre-cotyledonary-stage somatic embryos had approximately 50- and 100-fold more protein kinase activity than cotyledonary-stage somatic and zygotic embryos”
t0“alfalfa”
Claim · model-written summary
Precotyledonary somatic extracts had roughly 100-fold more kinase activity than zygotic embryo extracts.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- pre-cotyledonary-stage somatic embryose0
- Outcome
- protein kinase activitye0
- Direction
- moree0
- Value
- 100-folde0
- Comparator
- zygotic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cell-free-translation extracts from pre-cotyledonary-stage somatic embryos had approximately 50- and 100-fold more protein kinase activity than cotyledonary-stage somatic and zygotic embryos”
t0“alfalfa”
Claim · model-written summary
Some phosphorylated polypeptides were stage-specific.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- polypeptides were phosphorylatede0
- Qualifier
- unique to the early stage and some to the late-stagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Several polypeptides were phosphorylated; some of them were unique to the early stage and some to the late-stage developing embryos”
t0“alfalfa”
Claim · model-written summary
A 65 kDa protein was heavily phosphorylated in precotyledonary somatic embryos.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- pre-cotyledonary-stage somatic embryose0
- Gene
- 65 kDa proteine0
- Outcome
- phosphorylated heavilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 65 kDa protein was phosphorylated heavily in pre-cotyledonary-stage somatic embryos”
t0“alfalfa”
Claim · model-written summary
The 65 kDa phosphorylated protein comprised three components, two heavily phosphorylated.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- three main components, two of which were phosphorylated heavilye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This phosphorylated protein was comprised of three main components, two of which were phosphorylated heavily”
t0“alfalfa”
Claim · model-written summary
Heat shock abolished existing kinase activity in embryos.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- Heat-shocke0
- Outcome
- exitant kinase activitye0
- Direction
- loste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heat-shock treated-embryos lost their exitant kinase activity and at the same time another form of protein kinase activity was activated which phosphorylated a novel 28 kDa protein”
t0“alfalfa”
Claim · model-written summary
Heat shock activated another kinase form that phosphorylated a novel 28 kDa protein.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- Heat-shocke0
- Gene
- 28 kDa proteine0
- Outcome
- another form of protein kinase activity was activated which phosphorylated a novel 28 kDa proteine0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Heat-shock treated-embryos lost their exitant kinase activity and at the same time another form of protein kinase activity was activated which phosphorylated a novel 28 kDa protein”
t0“alfalfa”
Claim · model-written summary
Polysomal and non-polysomal mRNP fractions showed kinase activity on 65, 43 and 30 kDa proteins.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- Endogenous protein kinase activitye0
- Qualifier
- phosphorylated only 65, 43 and 30 kDa proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Endogenous protein kinase activity was also observed within the mRNPs of polysomal and non-polysomal fractions of developing embryos, and this phosphorylated only 65, 43 and 30 kDa proteins within these fractions”
t0“alfalfa”
Claim · model-written summary
A precotyledonary 30 kDa protein had greater phosphate-accepting affinity than later-stage proteins.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- pre-cotyledonary-stage somatic embryose0
- Gene
- 30 kDa proteine0
- Outcome
- affinity for accepting phosphate groupse0
- Direction
- highere0
- Comparator
- proteins from cotyledonary-stage somatic or zygotic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 30 kDa protein from the pre-cotyledonary-stage somatic embryos showed a higher affinity for accepting phosphate groups than the proteins from cotyledonary-stage somatic or zygotic embryos”
t0“alfalfa”
Claim · model-written summary
Kinase activity was largely independent of c-AMP.
- Taxon
- alfalfaMedicago sativat0
- Compound
- c-AMPe0
- Outcome
- activity of protein kinasee0
- Direction
- c-AMP-independente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of protein kinase was largely c-AMP-independent, but was dependent on Ca2+, phospholipid and phorbol ester”
t0“alfalfa”
Claim · model-written summary
Kinase activity depended on Ca2+, phospholipid and phorbol ester.
- Taxon
- alfalfaMedicago sativat0
- Compound
- Ca2+, phospholipid and phorbol estere0
- Outcome
- activity of protein kinasee0
- Direction
- dependente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of protein kinase was largely c-AMP-independent, but was dependent on Ca2+, phospholipid and phorbol ester”
t0“alfalfa”
Claim · model-written summary
The enzyme belongs to the protein kinase-C family.
- Taxon
- alfalfaMedicago sativat0
- Gene
- protein kinase-Ce0
- Outcome
- belongs to the protein kinase-C familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme belongs to the protein kinase-C family”
t0“alfalfa”
Claim · model-written summary
The 65 kDa protein cross-reacted with kinase-C antibodies.
- Taxon
- alfalfaMedicago sativat0
- Gene
- 65 kDa proteine0
- Outcome
- cross-reacts with antibodies made against protein kinase-C (alpha- and beta-isoforms)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 65 kDa protein cross-reacts with antibodies made against protein kinase-C (alpha- and beta-isoforms)”
t0“alfalfa”
Claim · model-written summary
The 65 kDa protein may be autophosphorylated.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- autophosphorylated proteine0
- Qualifier
- may bee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it may be an autophosphorylated protein”
t0“alfalfa”
The same questions in other papers
heat · physiology: enzyme activity · Medicago sativa (alfalfa)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.