Abstract
Cell suspensions of twelve citrus genotypes were successfully cryopreserved by vitrification. All genotypes survived cryopreservation with >90% viability and the surviving cells of some genotypes regenerated somatic embryos better than the controls. Single-cell sibling lines of cultivar Newhall were used for cytological and molecular examination. It was found that the ploidy constitution remained genetically stable and that no DNA sequence variation was detected by randomly amplified polymorphic DNA (RAPD) assay after cryopreservation. In addition, the methylation sensitive amplified polymorphism (MSAP) assay indicated that cryopreservation caused a significant change in DNA methylation status.
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Claims 5
Claim · model-written summary
All citrus genotypes survived cryopreservation with more than 90% viability.
CitrusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All genotypes survived cryopreservation with >90% viability”
t0“citrus”
e1“citrus”
Claim · model-written summary
Surviving cells of some genotypes regenerated somatic embryos better than controls.
- Treatment
- cryopreservationt0
- Outcome
- regenerated somatic embryose0
- Direction
- better thane0
- Qualifier
- some genotypese0
- Comparator
- the controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the surviving cells of some genotypes regenerated somatic embryos better than the controls.”
t0“cryopreservation”
t1“citrus”
e1“Cell suspensions of twelve citrus genotypes”
Claim · model-written summary
Ploidy constitution remained stable following cryopreservation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ploidy constitution remained genetically stable”
t0“cryopreservation”
t1“citrus”
e1“Cell suspensions of twelve citrus genotypes”
Claim · model-written summary
No DNA sequence variation was detected after cryopreservation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no DNA sequence variation was detected by randomly amplified polymorphic DNA (RAPD) assay after cryopreservation.”
t0“citrus”
e1“citrus”
Claim · model-written summary
Cryopreservation significantly changed DNA methylation status.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cryopreservation caused a significant change in DNA methylation status.”
t0“citrus”
e1“citrus”
The same questions in other papers
in vitro · growth · CitrusPositive and negative results
13 papers in the same direction as this paper · 7 in the opposite direction
- negative
- no effect
- negative
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.