Paper · 1986 · Journal of the American Society for Horticultural Science

Influence of Stock Plant Propagation Method on Tissue Culture and Leaf-bud Propagation of ‘Northblue’ Blueberry

Jeanne M. Grout; Paul E. Read

10
claims
20
verbatim quotes
12
citations
3
research areas
✓
Open access · cc-by-nc-nd

Abstract

Abstract The propagation method and vegetative condition of ‘Northblue’ blueberry ( Vaccinium corymbosum L.) stock plants influenced microshoot production in vitro and root formation on leaf-bud cuttings. In tissue culture (TC), explants from TC-derived stock plants produced longer shoots than explants from leaf-bud, standard- (ST-) derived stock on either Zimmerman’s (Zimm) medium at pH 4.8, Lloyd and McCown’s woody plant medium (WPM) at pH 4.8 or pH 5.2. Microshoots from explants of TC-stock plants also rooted more readily. Microshoot rootability decreased after 18 weeks on medium containing 68.6 μmol (12 mg/liter) 2iP. Microshoot production and rootability increased after 3 additional weeks on Zimm medium without 2iP present. Leaf-bud cuttings of ‘Northblue’ TC-stock plants treated with 5% and 10% concentrations of a commercial rooting compound (Dip-n-Grow) had a slightly higher rooting percentage and root rating than nontreated cuttings from ST-stock plants. However, cuttings from ST-stock plants of the same age showed larger increases in root formation and percentage of rooting in response to the same rooting compound treatments. Leaf-bud cuttings from vegetative TC-stock plants that developed shoots had more basal branches than those from floral ST-stock plants. Branch elongation was greatest and basal branches fewest on cuttings from floral ST stock plants. Successful propagation with cuttings and in vitro explants may be related to the condition of the stock plants, which have been altered by their own propagation methods and the plant growth regulators applied. Chemical names used: 1 H -indole-3-acetic acid (IAA); 1 H -indole-3-butanoic acid (IBA); 1-naphthaleneacetic acid (NAA); N -(3-methyl-2-butenyl)-2 H -purin-6-amine (2iP).

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Claims 10

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Explants from TC-derived stock plants produced longer shoots than explants from leaf-bud, standard-derived stock on Zimmerman medium at pH 4.8, woody plant medium at pH 4.8 or pH 5.2.

Taxon
Blueberryt0
Treatment
explants from TC-derived stock plantse0
Outcome
shootse0
Direction
longere0
Qualifier
on either Zimmerman’s (Zimm) medium at pH 4.8, Lloyd and McCown’s woody plant medium (WPM) at pH 4.8 or pH 5.2e0
Comparator
explants from leaf-bud, standard- (ST-) derived stocke0

Verbatim

e0

“In tissue culture (TC), explants from TC-derived stock plants produced longer shoots than explants from leaf-bud, standard- (ST-) derived stock on either Zimmerman’s (Zimm) medium at pH 4.8, Lloyd and McCown’s woody plant medium (WPM) at pH 4.8 or pH 5.2”

✓ located verbatim in the source · abstract, characters 205–459 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Microshoots from explants of TC-stock plants rooted more readily.

Taxon
Blueberryt0
Treatment
explants of TC-stock plantse0
Outcome
rootede0
Direction
more readilye0

Not reportedComparator

Verbatim

e0

“Microshoots from explants of TC-stock plants also rooted more readily”

✓ located verbatim in the source · abstract, characters 461–530 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Microshoot rootability decreased after 18 weeks on medium containing 68.6 μmol (12 mg/liter) 2iP.

Taxon
Blueberryt0
Treatment
18 weeks on medium containing 68.6 μmol (12 mg/liter) 2iPe0
Outcome
Microshoot rootabilitye0
Direction
decreasede0

Not reportedComparator

Verbatim

e0

“Microshoot rootability decreased after 18 weeks on medium containing 68.6 μmol (12 mg/liter) 2iP”

✓ located verbatim in the source · abstract, characters 532–628 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Microshoot production and rootability increased after 3 additional weeks on Zimm medium without 2iP.

Taxon
Blueberryt0
Treatment
3 additional weeks on Zimm medium without 2iP presente0
Outcome
Microshoot production and rootabilitye0
Direction
increasede0

Not reportedComparator

Verbatim

e0

“Microshoot production and rootability increased after 3 additional weeks on Zimm medium without 2iP present”

✓ located verbatim in the source · abstract, characters 630–737 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaf-bud cuttings of TC-stock plants treated with 5% and 10% rooting compound (Dip-n-Grow) had slightly higher rooting percentage and root rating than nontreated cuttings from ST-stock plants.

Taxon
Blueberryt0
Treatment
5% and 10% concentrations of a commercial rooting compound (Dip-n-Grow)e0
Outcome
rooting percentage and root ratinge0
Direction
highere0
Qualifier
slightlye0
Comparator
nontreated cuttings from ST-stock plantse0

Verbatim

e0

“Leaf-bud cuttings of ‘Northblue’ TC-stock plants treated with 5% and 10% concentrations of a commercial rooting compound (Dip-n-Grow) had a slightly higher rooting percentage and root rating than nontreated cuttings from ST-stock plants”

✓ located verbatim in the source · abstract, characters 739–975 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cuttings from ST-stock plants of the same age showed larger increases in root formation and rooting percentage in response to the same rooting compound treatments.

Taxon
Blueberryt0
Treatment
the same rooting compound treatmentse0
Outcome
root formation and percentage of rootinge0
Direction
larger increasese0

Not reportedComparator

Verbatim

e0

“cuttings from ST-stock plants of the same age showed larger increases in root formation and percentage of rooting in response to the same rooting compound treatments”

✓ located verbatim in the source · abstract, characters 986–1151 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Leaf-bud cuttings from vegetative TC-stock plants that developed shoots had more basal branches than those from floral ST-stock plants.

Taxon
Blueberryt0
Treatment
vegetative TC-stock plants that developed shootse0
Outcome
basal branchese0
Direction
moree0
Comparator
floral ST-stock plantse0

Verbatim

e0

“Leaf-bud cuttings from vegetative TC-stock plants that developed shoots had more basal branches than those from floral ST-stock plants”

✓ located verbatim in the source · abstract, characters 1153–1287 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Branch elongation was greatest on cuttings from floral ST stock plants.

Taxon
Blueberryt0
Treatment
floral ST stock plantse0
Outcome
Branch elongatione0
Direction
greateste0

Not reportedComparator

Verbatim

e0

“Branch elongation was greatest and basal branches fewest on cuttings from floral ST stock plants”

✓ located verbatim in the source · abstract, characters 1289–1385 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Basal branches were fewest on cuttings from floral ST stock plants.

Taxon
Blueberryt0
Treatment
floral ST stock plantse0
Outcome
basal branchese0
Direction
feweste0

Not reportedComparator

Verbatim

e0

“Branch elongation was greatest and basal branches fewest on cuttings from floral ST stock plants”

✓ located verbatim in the source · abstract, characters 1289–1385 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑
Mechanismhypothesisoriginal resultmeasured

Claim · model-written summary

The authors suggest successful propagation with cuttings and in vitro explants may be related to the condition of the stock plants.

Taxon
Blueberryt0
Outcome
Successful propagatione0
Qualifier
the condition of the stock plantse0

Verbatim

e0

“Successful propagation with cuttings and in vitro explants may be related to the condition of the stock plants”

✓ located verbatim in the source · abstract, characters 1387–1497 · show in text ↑
t0

“Blueberry”

✓ located verbatim in the source · title, characters 102–111 · show in text ↑

in vitro · yield · VacciniumPositive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive
See all 4 papers →

in vitro · growth · VacciniumPositive and negative results

12 papers: 10 positive, 2 negative or no effect

9 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

8 papers more in this group

See all 12 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.