Abstract
The influence of nitrogen form on bentazon uptake and toxicity to soybean cell cultures and medium pH was examined. Soybean cell growth was completely inhibited at 40 μM bentazon when cells were cultured in a Murashige and Skoog medium containing 20 mM ammonium and 38 mM nitrate. However, bentazon, up to 100 μM, did not affect the growth of cells cultured in B5 medium (Gamborg B5 medium containing 2 mM ammonium and 25 mM nitrate). Twenty-minute 14 C-bentazon uptake experiments on 48- and 96-h-old cells demonstrated that 14 C uptake was nitrogen-type dependent, with cells cultured in AMM medium (B5 medium containing 38 mM nitrate and 12 mM ammonium) accumulating 5 to 10 times more 14 C than those in B5 or NIT media (B5 containing 50 mM nitrate). Differential bentazon uptake between ammonium and nitrate-containing media appears to be attributable to the effect of nitrogen form on pH. From an initial pH of 5.5, the pH of AMM medium decreased to 4 by 48 h. The pH of NIT or B5 medium increased to approximately 7 from 5.5. Changes in pH are likely attributable to cellular maintenance of ionic balance; NH 4 + and NO 3 − uptake results in H+ and OH- efflux, respectively. Methylamine at 12 mM did not result in a decrease in medium pH. Bentazon, a weak acid (pKa 3.3), would be expected to penetrate the plasma-membrane faster in the molecular form at low pH. Support for a nitrogen pH theory is provided by the finding that 14 C-bentazon uptake was similar for ammonium and nitrate-containing media when media were buffered.
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Claims 7
Claim · model-written summary
At 40 μM bentazon, soybean cell growth was completely inhibited in Murashige and Skoog medium.
- Taxon
- Soybean
Glycine maxe0 - Treatment
- 40 μM bentazone0
- Outcome
- cell growthe0
- Direction
- completely inhibitede0
- Setting
- Murashige and Skoog mediume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Soybean cell growth was completely inhibited at 40 μM bentazon when cells were cultured in a Murashige and Skoog medium containing 20 mM ammonium and 38 mM nitrate.”
Claim · model-written summary
Bentazon up to 100 μM did not affect soybean cell growth in B5 medium.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- bentazon, up to 100 μMe0
- Outcome
- growth of cellse0
- Direction
- did not affecte0
- Setting
- B5 mediume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bentazon, up to 100 μM, did not affect the growth of cells cultured in B5 medium (Gamborg B5 medium containing 2 mM ammonium and 25 mM nitrate).”
t0“Soybean (Glycine max)”
Claim · model-written summary
Soybean cells grown in AMM medium accumulated 5 to 10 times more radiolabeled bentazon than cells in B5 or NIT media.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- AMM mediume0
- Outcome
- 14 Ce0
- Direction
- moree0
- Value
- 5 to 10 timese0
- Comparator
- B5 or NIT mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cells cultured in AMM medium (B5 medium containing 38 mM nitrate and 12 mM ammonium) accumulating 5 to 10 times more 14 C than those in B5 or NIT media (B5 containing 50 mM nitrate)”
t0“Soybean (Glycine max)”
Claim · model-written summary
AMM medium pH fell from 5.5 to 4 by 48 hours with soybean cells.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“From an initial pH of 5.5, the pH of AMM medium decreased to 4 by 48 h.”
t0“Soybean (Glycine max)”
Claim · model-written summary
NIT and B5 medium pH rose from 5.5 to approximately 7 with soybean cells.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- pH of NIT or B5 mediume0
- Direction
- increasede0
- Value
- approximately 7e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The pH of NIT or B5 medium increased to approximately 7 from 5.5.”
t0“Soybean (Glycine max)”
Claim · model-written summary
Methylamine at 12 mM did not decrease soybean culture medium pH.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Methylamine at 12 mMe0
- Outcome
- medium pHe0
- Direction
- did not result in a decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methylamine at 12 mM did not result in a decrease in medium pH.”
t0“Soybean (Glycine max)”
Claim · model-written summary
Buffered ammonium and nitrate media produced similar radiolabeled bentazon uptake in soybean cells.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- ammoniume0
- Outcome
- 14 C-bentazon uptakee0
- Direction
- similare0
- Qualifier
- when media were bufferede0
- Comparator
- nitrate-containing mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“14 C-bentazon uptake was similar for ammonium and nitrate-containing media when media were buffered.”
t0“Soybean (Glycine max)”
The same questions in other papers
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See all 94 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.