Abstract
The efficiency of maize pollen induced doubled haploid (DH) production in durum wheat is low mainly because of poor germination of haploid embryos on culture media. The objective of this research was to investigate the effect of culture media supplements on DH production in durum wheat. Haploid durum embryos generated by the wheat × maize method from the cultivar Transcend and F1 plants of a cross were cultured on a basic medium supplemented with 1 mg L−1 3,6-dichloro-o-anisic acid (dicamba), 1 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D), 100 mg L−1 silver nitrate (AgNO3), 1 mg L−1 6-benzylaminopurine (BAP), 1 mg L−1 indole acetic acid (IAA), 1 mg L−1 naphthalene acetic acid (NAA), or 10 mg L−1 ascorbic acid. The proportion of haploid plantlets per embryo increased significantly by 33% with the AgNO3 supplemented medium over the basic medium, while the increase in proportion of doubled plants per embryo of 20% was not significant. The proportion of haploid plantlets was improved with BAP in the embryos of the F1 plants, but the proportion of haploid plantlets and DHs per embryo produced with ascorbic acid and IAA were not different from the basic medium. Dicamba and NAA produced results less favorable than the control, and no germination occurred with 2,4-D. The advantage of AgNO3 was attributed to a reduction in the microbial contamination of embryos.
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Claims 9
Claim · model-written summary
Silver nitrate supplementation increased haploid plantlets per embryo by 33% over basic medium.
- Taxon
- durum wheat
Triticum turgidum subsp. durumt0 - Treatment
- AgNO3 supplemented mediume0
- Outcome
- proportion of haploid plantlets per embryoe0
- Direction
- increasede0
- Value
- 33%e0
- Comparator
- basic mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proportion of haploid plantlets per embryo increased significantly by 33% with the AgNO3 supplemented medium over the basic medium”
t0“durum wheat”
Claim · model-written summary
Silver nitrate raised doubled plants per embryo by 20%, without statistical significance.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Treatment
- AgNO3 supplemented mediume0
- Outcome
- proportion of doubled plants per embryoe0
- Direction
- increasee0
- Value
- 20%e0
- Comparator
- basic mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proportion of haploid plantlets per embryo increased significantly by 33% with the AgNO3 supplemented medium over the basic medium, while the increase in proportion of doubled plants per embryo of 20% was not significant.”
t0“durum wheat”
Claim · model-written summary
BAP improved haploid plantlet proportion in F1 embryos.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Material
- F1 plantse0
- Organ / stage
- embryose0
- Treatment
- BAPe0
- Outcome
- proportion of haploid plantletse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proportion of haploid plantlets was improved with BAP in the embryos of the F1 plants”
t0“durum wheat”
Claim · model-written summary
Ascorbic acid did not alter haploid plantlets or doubled haploids per embryo relative to basic medium.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Treatment
- ascorbic acide0
- Outcome
- proportion of haploid plantlets and DHs per embryoe0
- Direction
- not differente0
- Comparator
- basic mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the proportion of haploid plantlets and DHs per embryo produced with ascorbic acid and IAA were not different from the basic medium.”
t0“durum wheat”
Claim · model-written summary
IAA did not alter haploid plantlets or doubled haploids per embryo relative to basic medium.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Treatment
- IAAe0
- Outcome
- proportion of haploid plantlets and DHs per embryoe0
- Direction
- not differente0
- Comparator
- basic mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the proportion of haploid plantlets and DHs per embryo produced with ascorbic acid and IAA were not different from the basic medium.”
t0“durum wheat”
Claim · model-written summary
Dicamba performed less favorably than control.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Treatment
- Dicambae0
- Outcome
- results less favorablee0
- Direction
- less favorablee0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dicamba and NAA produced results less favorable than the control”
t0“durum wheat”
Claim · model-written summary
NAA performed less favorably than control.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Treatment
- NAAe0
- Outcome
- results less favorablee0
- Direction
- less favorablee0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dicamba and NAA produced results less favorable than the control”
t0“durum wheat”
Claim · model-written summary
No embryos germinated with 2,4-D.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Treatment
- 2,4-De0
- Outcome
- no germinatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no germination occurred with 2,4-D.”
t0“durum wheat”
Claim · model-written summary
The authors attributed the silver nitrate advantage to reduced microbial embryo contamination.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Treatment
- AgNO3e0
- Outcome
- advantagee0
- Qualifier
- reduction in the microbial contamination of embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The advantage of AgNO3 was attributed to a reduction in the microbial contamination of embryos.”
t0“durum wheat”
The same questions in other papers
2,4-D · growth · Triticum turgidum (rivet wheat)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.