Abstract
Crossing different ploidy grapes is an effective way to obtain new seedless cultivars. Although embryo rescue has been extensively applied in breeding seedless and triploid grapes, only a few improved cultivars have been developed. Based on preliminary studies, we set five crosses between tetraploid and diploid grape varieties to obtain new hybrid triploid germplasms. Additionally, we compared two different methods of performing in vitro embryo rescue and sowing in the development of hybrid triploid grape plants. The results showed that the germination rate of hybrid seeds was much lower (0-22.8%) than that of the self-pollinated seeds (50.9-61.2%) obtained though the same method of in vitro culture. Meanwhile, the seed germination rates of all crosses obtain through in vitro culture (0-61.2%) were higher than those obtained through sowing (0-42.0%). Identification of ploidy level confirmed that three lines obtained from the crosses of 'Ruby Seedless (2x) x Black Olympia (4x)' and 'Big black (2x) x Kyoho (4x)' were triploid, and one line from the cross of 'Big black (2x) x Kyoho (4x)' was haploid, and the others were diploid, tetraploid, or aneuploidy plants. Moreover, 4 haploid and 42 triploid surviving grape seedlings were planted in a vineyard after propagation. Therefore, an efficient system of breeding triploid seedless grapes using embryo rescue was established and 42 hybrid triploid germplasms were obtained for use in future studies.
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Claims 8
Claim · model-written summary
Hybrid grape seeds germinated less often than self-pollinated seeds in vitro.
Verbatim
e0“The results showed that the germination rate of hybrid seeds was much lower (0-22.8%) than that of the self-pollinated seeds (50.9-61.2%) obtained though the same method of in vitro culture.”
t0“grapes”
Claim · model-written summary
In vitro culture gave higher grape seed germination rates than sowing.
Verbatim
e0“Meanwhile, the seed germination rates of all crosses obtain through in vitro culture (0-61.2%) were higher than those obtained through sowing (0-42.0%).”
t0“grapes”
Claim · model-written summary
Three grape lines from the named crosses were triploid.
Verbatim
e0“Identification of ploidy level confirmed that three lines obtained from the crosses of 'Ruby Seedless (2x) x Black Olympia (4x)' and 'Big black (2x) x Kyoho (4x)' were triploid”
t0“grapes”
Claim · model-written summary
One line from the Big black × Kyoho cross was haploid.
Verbatim
e0“one line from the cross of 'Big black (2x) x Kyoho (4x)' was haploid”
t0“grapes”
Claim · model-written summary
Other progeny were diploid, tetraploid, or aneuploid.
Verbatim
e0“the others were diploid, tetraploid, or aneuploidy plants”
t0“grapes”
Claim · model-written summary
Four haploid grape seedlings survived and were planted in a vineyard after propagation.
Verbatim
e0“4 haploid and 42 triploid surviving grape seedlings were planted in a vineyard after propagation”
t0“grapes”
Claim · model-written summary
Forty-two triploid grape seedlings survived and were planted in a vineyard after propagation.
Verbatim
e0“4 haploid and 42 triploid surviving grape seedlings were planted in a vineyard after propagation”
t0“grapes”
Claim · model-written summary
The embryo rescue breeding system yielded 42 hybrid triploid germplasms.
Verbatim
e0“Therefore, an efficient system of breeding triploid seedless grapes using embryo rescue was established and 42 hybrid triploid germplasms were obtained for use in future studies.”
t0“grapes”
The same questions in other papers
in vitro · growth · VitisPositive and negative results
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