Abstract
Aegilops variabilis (UUSvSv), an important sources for wheat improvement, originated from chromosome doubling of a natural hybrid between Ae. umbellulata (UU) with Ae. longissima (SlSl). The Ae. variabilis karyotype was poorly characterized by fluorescent in situ hybridization (FISH). The FISH probe combination of pSc119.2, pTa71 and pTa-713 identified each of the 14 pairs of Ae. variabilis chromosomes. Our FISH ideogram was further used to detect an Ae. variabilis chromosome carrying stripe rust resistance in the background of wheat lines developed from crosses of the stripe rust susceptible bread wheat cultivar Yiyuan 2 with a resistant Ae. variabilis accession. Among the 15 resistant BC1F7 lines, three were 2Sv + 4Sv addition lines (2n = 46) and 12 were 2Sv(2B) or 2Sv(2D) substitution lines that were confirmed with SSR markers. SSR marker gwm148 can be used to trace 2Sv in common wheat background. Chromosome 2Sv probably carries gametocidal(Gc) gene(s) since cytological instability and chromosome structural variations, including non-homologous translocations, were observed in some lines with this chromosome. Due to the effects of photoperiod genes, substitution lines 2Sv(2D) and 2Sv(2B) exhibited late heading with 2Sv(2D) lines being later than 2Sv(2B) lines. 2Sv(2D) substitution lines were also taller and exhibited higher spikelet numbers and longer spikes.
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Claims 9
Claim · model-written summary
The FISH probes identified all 14 Aegilops variabilis chromosome pairs.
- Taxon
- Aegilops variabilisAegilops peregrinat0
- Outcome
- Ae. variabilis chromosomese0
- Value
- 14 pairse0
Verbatim
e0“The FISH probe combination of pSc119.2, pTa71 and pTa-713 identified each of the 14 pairs of Ae. variabilis chromosomes.”
t0“Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat”
Claim · model-written summary
The FISH ideogram detected a resistance-carrying Aegilops chromosome in wheat lines.
- Taxon
- wheat
Triticum aestivume0 - Outcome
- Ae. variabilis chromosome carrying stripe rust resistancee0
- Comparator
- wheat linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our FISH ideogram was further used to detect an Ae. variabilis chromosome carrying stripe rust resistance in the background of wheat lines”
t0“Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat”
Claim · model-written summary
Three resistant BC1F7 lines were 2Sv + 4Sv addition lines.
- Taxon
- wheatTriticum aestivumt0
- Material
- BC1F7 linese0
- Outcome
- 2Sv + 4Sv addition linese0
- Value
- threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the 15 resistant BC1F7 lines, three were 2Sv + 4Sv addition lines (2n = 46)”
t0“Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat”
Claim · model-written summary
Twelve resistant BC1F7 lines were 2Sv substitution lines.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- 2Sv(2B) or 2Sv(2D) substitution linese0
- Value
- 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“12 were 2Sv(2B) or 2Sv(2D) substitution lines that were confirmed with SSR markers.”
t0“Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat”
Claim · model-written summary
SSR marker gwm148 can trace 2Sv in common wheat.
- Taxon
- common wheatTriticum aestivume0
- Gene
- gwm148e0
- Outcome
- trace 2Sve0
- Comparator
- common wheat backgrounde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SSR marker gwm148 can be used to trace 2Sv in common wheat background.”
t0“Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat”
Claim · model-written summary
Chromosome 2Sv probably carries gametocidal genes, based on cytological instability and structural variation.
- Taxon
- wheatTriticum aestivumt0
- Gene
- gametocidal(Gc) gene(s)e0
- Outcome
- cytological instability and chromosome structural variationse0
- Qualifier
- probablye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chromosome 2Sv probably carries gametocidal(Gc) gene(s) since cytological instability and chromosome structural variations, including non-homologous translocations, were observed in some lines with this chromosome.”
t0“Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat”
Claim · model-written summary
2Sv(2D) and 2Sv(2B) substitution lines headed late.
- Taxon
- wheatTriticum aestivumt0
- Material
- 2Sv(2D) and 2Sv(2B)e0
- Outcome
- late headinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“substitution lines 2Sv(2D) and 2Sv(2B) exhibited late heading”
t0“Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat”
Claim · model-written summary
2Sv(2D) lines headed later than 2Sv(2B) lines.
- Taxon
- wheatTriticum aestivumt0
- Material
- 2Sv(2D)e0
- Outcome
- latere0
- Comparator
- 2Sv(2B)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2Sv(2D) lines being later than 2Sv(2B) lines.”
t0“Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat”
Claim · model-written summary
2Sv(2D) lines were taller and had higher spikelet numbers and longer spikes.
- Taxon
- wheatTriticum aestivumt0
- Material
- 2Sv(2D)e0
- Outcome
- taller and exhibited higher spikelet numbers and longer spikese0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“2Sv(2D) substitution lines were also taller and exhibited higher spikelet numbers and longer spikes.”
t0“Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat”