Abstract
The relationship between aluminium (Al) uptake and growth inhibition was studied in tobacco cells (Nicotiana tabacum L. cv. Samsun; nonchlorophyllic cell line) in suspension culture. Cells at the logarithmic phase of growth were treated with 100 μM A1C13 in modified Murashige-Skoog medium prepared without Pi and EDTA (pH 4.0) for up to 21 h. After treatment, the inhibition of cell growth by Al was estimated from the growth of the Al-treated cells relative to that of the control cells during post-treatment culture. Neither Al uptake nor the inhibition of the growth occurred with less than a 10-h exposure but then both parameters increased rapidly, reaching maximum values after an 18-h exposure. When cells were treated with AlCl3 at various concentrations for 18 h, the extent of growth inhibition was found to be a function of the Al content of the cells. The dose-response curve (Al uptake versus growth inhibition) resembled the curve expected for “single-hit” kinetics. Extrapolation from the curve suggested that the uptake of 1 × 1011 Al atoms per cell is the minimum dose that inhibits cell growth. Cells of stationary phase were resistant to Al and did not take up Al, an indication that the uptake of Al depends on the active growth of cells. Results of several types of experiment (hematoxylin staining, washing with chelators, digestion of cell walls) indicated that Al was incorporated inside the cells. Together, therefore, our results suggest that the amount of Al absorbed by the cells is a determining factor in the inhibition of growth by Al.
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Claims 5
Claim · model-written summary
Neither aluminium uptake nor growth inhibition occurred before 10 hours of exposure.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- Ale0
- Outcome
- Al uptake nor the inhibition of the growthe0
- Direction
- Neithere0
- Qualifier
- less than a 10-h exposuree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither Al uptake nor the inhibition of the growth occurred with less than a 10-h exposure”
t0“Tobacco”
Claim · model-written summary
Aluminium uptake and growth inhibition rose rapidly after 10 hours and peaked after 18 hours.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- 18-h exposuree0
- Outcome
- both parameterse0
- Direction
- increased rapidlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both parameters increased rapidly, reaching maximum values after an 18-h exposure.”
t0“Tobacco”
Claim · model-written summary
Growth inhibition was a function of cellular aluminium content after 18 hours of treatment.
- Taxon
- TobaccoNicotiana tabacumt0
- Compound
- Al content of the cellse0
- Outcome
- extent of growth inhibitione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the extent of growth inhibition was found to be a function of the Al content of the cells.”
t0“Tobacco”
Claim · model-written summary
Stationary-phase tobacco cells resisted aluminium and did not take it up.
- Taxon
- TobaccoNicotiana tabacumt0
- Organ / stage
- stationary phasee0
- Outcome
- resistant to Al and did not take up Ale0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cells of stationary phase were resistant to Al and did not take up Al”
t0“Tobacco”
Claim · model-written summary
Aluminium was incorporated inside tobacco cells.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- Al was incorporated inside the cellse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Al was incorporated inside the cells.”
t0“Tobacco”
The same questions in other papers
aluminium atom · disease · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.