Abstract
There is need for backup storage of clonally propagated plant cultivars of numerous taxa. Initial tests, using a protocol developed for dormant apple buds that includes desiccation and slow freezing prior to immersion in liquid nitrogen (-196 C), was not effective with `Valiant' grape. Accordingly, replicates of V. vinifera `Riesling', V. riparia, `Valiant' and a V. amurensis × riparia cross were also tested for survival at –196 C, following desiccation to 25% & 18% water (fwb) and direct immersion into liquid nitrogan. Visual and electrolyte leakage ratings following nine days of dehydration in moist peat were used to assess viability. Direct immersion of desiccated samples resulted in survival for some buds of `Valiant' and a V. amurensis × riparia cross. V. riparia showed some survival when field hydrated and at 25% water, while all buds desiccated to 18% survived. `Riesling' did not survive desiccation, and was killed by all -196 C treatments. The apple protocol was partially effective, in combination with desiccation to 18% in `Valiant' and V. riparia. This is the first report of grape bud survival in liquid nitrogen and more detailed studies are planned.
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Claims 7
Claim · model-written summary
was not effective with `Valiant' grape
Not reportedComparator
Verbatim
e0“was not effective with `Valiant' grape”
Claim · model-written summary
Direct immersion of desiccated samples resulted in survival for some buds of `Valiant'
Not reportedComparatorValue
Verbatim
e0“Direct immersion of desiccated samples resulted in survival for some buds of `Valiant'”
t0“GRAPE”
Claim · model-written summary
V. riparia showed some survival when field hydrated and at 25% water
- Taxon
- V. riparia
Vitis ripariae0 - Outcome
- some survivale0
- Value
- 25% watere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“V. riparia showed some survival when field hydrated and at 25% water”
Claim · model-written summary
all buds desiccated to 18% survived
Verbatim
e0“all buds desiccated to 18% survived”
t0“GRAPE”
Claim · model-written summary
`Riesling' did not survive desiccation
Verbatim
e0“`Riesling' did not survive desiccation”
t0“GRAPE”
Claim · model-written summary
`Riesling' did not survive desiccation, and was killed by all -196 C treatments
Verbatim
e0“`Riesling' did not survive desiccation, and was killed by all -196 C treatments”
t0“GRAPE”
Claim · model-written summary
The apple protocol was partially effective, in combination with desiccation to 18% in `Valiant'
Verbatim
e0“The apple protocol was partially effective, in combination with desiccation to 18% in `Valiant'”
t0“GRAPE”