Abstract
The details of a convenient laboratory press are presented and a procedure by which plant sap is readily obtained is described. The pressing procedure has been shown to release 53–74 per cent. of the total plant water as expressed sap. Preliminary observations on the uptake and translocation of antibiotics by higher plants obtained by examining expressed sap are described. It is shown that the assay of expressed sap provides a measure of the griseofulvin and chloramphenicol concentration in leaves of plants grown in solutions of these antibiotics that does not differ significantly from the value obtained by the assay of water extracts. Extraction of leaves from plants grown in griseofulvin solutions with organic solvents demonstrates a much greater antibiotic content than is indicated by the assay of expressed sap. To explain this difference a ‘free’ antibiotic fraction, obtainable in expressed sap or water extracts, and a ‘bound’ fraction recovered from the leaf only by organic solvent extraction are postulated.
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Claims 5
Claim · model-written summary
Pressing released 53–74 per cent. of the total plant water of higher plants as expressed sap.
- Taxon
- higher plantsEmbryophytae0
- Outcome
- total plant watere1
- Value
- 53–74 per cent.e1
Verbatim
e0“Preliminary observations on the uptake and translocation of antibiotics by higher plants”
e1“The pressing procedure has been shown to release 53–74 per cent. of the total plant water as expressed sap”
Claim · model-written summary
Assay of expressed sap gives a measure of griseofulvin and chloramphenicol concentration in leaves that does not differ significantly from water extracts.
- Taxon
- higher plantsEmbryophytae0
- Treatment
- plants grown in solutions of these antibioticse2
- Outcome
- griseofulvin and chloramphenicol concentration in leavese2
- Comparator
- water extractse2
Verbatim
e0“Preliminary observations on the uptake and translocation of antibiotics by higher plants”
e2“the assay of expressed sap provides a measure of the griseofulvin and chloramphenicol concentration in leaves of plants grown in solutions of these antibiotics that does not differ significantly from the value obtained by the assay of water extracts”
Claim · model-written summary
Solvent extraction of leaves shows a much greater antibiotic content of griseofulvin-grown plants than the expressed sap assay.
Verbatim
e0“Preliminary observations on the uptake and translocation of antibiotics by higher plants”
e3“Extraction of leaves from plants grown in griseofulvin solutions with organic solvents demonstrates a much greater antibiotic content than is indicated by the assay of expressed sap”
Claim · model-written summary
A free antibiotic fraction and a bound antibiotic fraction are postulated to explain the difference between extraction methods in leaves.
- Taxon
- higher plantsEmbryophytae0
- Outcome
- differencee4
- Qualifier
- ‘free’ antibiotic fractione4
Verbatim
e0“Preliminary observations on the uptake and translocation of antibiotics by higher plants”
e4“To explain this difference a ‘free’ antibiotic fraction, obtainable in expressed sap or water extracts, and a ‘bound’ fraction recovered from the leaf only by organic solvent extraction are postulated”
Claim · model-written summary
Preliminary observations describe uptake and translocation of antibiotics by higher plants.
- Taxon
- higher plantsEmbryophytae0
- Outcome
- uptake and translocation of antibioticse0
Verbatim
e0“Preliminary observations on the uptake and translocation of antibiotics by higher plants”