Abstract
A study was conducted to develop protocol for the preservation of pecan genetic variability by cryogenic storage of zygotic embryos and subsequent in vitro plant regeneration. Parameters evaluated for their influence on embryo survival included the amount of intact kernel, liquid nitrogen (LN) treatment, desiccation, and genotype specificity. Optimum germination with minimum contamination occurred with 12% of the kernel intact. Treatment of explants with LN reduced the percentage of embryos developing into intact plants. `Curtis' and `Shoshoni' had a significantly higher morphogenic response in shoots only than all other cultivars. In summary, cryogenic storage of pecan zygotic embryos was determined to be a feasible means for preservation of pecan germplasm. However, the procedures used in the current study should be altered to increase the probability of embryo survival.
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Claims 6
Claim · model-written summary
Optimum germination with minimum contamination occurred when 12% of the kernel remained intact.
- Taxon
- PECAN
_1_(24790682337).jpg?width=60)
Carya illinoinensist0 - Treatment
- 12% of the kernel intacte0
- Outcome
- Optimum germination with minimum contaminatione0
- Direction
- Optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_1_(24790682337).jpg?width=500)
Verbatim
e0“Optimum germination with minimum contamination occurred with 12% of the kernel intact.”
t0“PECAN”
Claim · model-written summary
Liquid nitrogen reduced the percentage of embryos developing into intact plants.
- Taxon
- PECANCarya illinoinensist0
- Treatment
- LNe0
- Outcome
- percentage of embryos developing into intact plantse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of explants with LN reduced the percentage of embryos developing into intact plants.”
t0“PECAN”
Claim · model-written summary
Curtis had a significantly higher shoots-only morphogenic response than other cultivars.
- Taxon
- PECANCarya illinoinensist0
- Material
- `Curtis'e0
- Outcome
- morphogenic response in shoots onlye0
- Direction
- highere0
- Comparator
- all other cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“`Curtis' and `Shoshoni' had a significantly higher morphogenic response in shoots only than all other cultivars.”
t0“PECAN”
Claim · model-written summary
Shoshoni had a significantly higher shoots-only morphogenic response than other cultivars.
- Taxon
- PECANCarya illinoinensist0
- Material
- `Shoshoni'e0
- Outcome
- morphogenic response in shoots onlye0
- Direction
- highere0
- Comparator
- all other cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“`Curtis' and `Shoshoni' had a significantly higher morphogenic response in shoots only than all other cultivars.”
t0“PECAN”
Claim · model-written summary
Cryogenic storage of pecan zygotic embryos was feasible for germplasm preservation.
- Taxon
- pecanCarya illinoinensise0
- Outcome
- feasible means for preservation of pecan germplasme0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cryogenic storage of pecan zygotic embryos was determined to be a feasible means for preservation of pecan germplasm”
Claim · model-written summary
The authors recommend altering the procedures to improve embryo survival.
- Taxon
- PECANCarya illinoinensist0
- Outcome
- increase the probability of embryo survivale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the procedures used in the current study should be altered to increase the probability of embryo survival”
t0“PECAN”