Paper · 1983

Quantitative Measurements of Phosphofructokinase in the Shoot Apical Meristem, Leaf Primordia, and Leaf Tissues of Dianthus

Judith G. Croxdale

8
claims
17
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Phosphofructokinase (EC 2.7.1.11) activity in the apical meristem, leaf primordia, and leaf tissues (epidermis, mesophyll, xylem, phloem) of Dianthus chinensis L. was determined. These data show that activity on a dry weight per hour basis was about 1000 millimoles in the apical meristem and two youngest primordia, and declined to 150 millimoles in the mesophyll of the sixth leaf pair and to 80 millimoles in older leaves just expanded out of the bud. On a cell per hour basis, the activity increased from 75 femtomoles in cells of the apical meristem and leaf primordia to 1.5 picomoles in the mesophyll of the sixth leaf pair. However, on a cell volume (cubic micrometers) per hour basis, the opposite was true. The results indicate that (a) on a dry weight or unit cell volume basis the capacity for glycolysis declines during leaf development and that (b) within the apical meristem, incipient primordia are not associated with phosphofructokinase activity greater than adjacent areas of the meristem. In comparison to glucose-6-phosphate dehydrogenase activity previously measured in equivalent samples (Croxdale, Outlaw 1983 Planta 157: 289-297), the capacity of the glycolytic pathway is greater than the pentose phosphate pathway in the apical meristem

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Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Phosphofructokinase activity was about 1000 millimoles per dry weight per hour in the apical meristem and two youngest primordia.

Taxon
Dianthus chinensisDianthus chinensise0
Organ / stage
apical meristem and two youngest primordiae1
Outcome
activitye1
Value
about 1000 millimolese1
Qualifier
on a dry weight per hour basise1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus chinensis
Dianthus chinensis · Taxon · picture from its Wikimedia Commons categoryGitartha.bordoloi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Phosphofructokinase (EC 2.7.1.11) activity in the apical meristem, leaf primordia, and leaf tissues (epidermis, mesophyll, xylem, phloem) of Dianthus chinensis L. was determined”

✓ located verbatim in the source · abstract, characters 0–177 · show in text ↑
e1

“These data show that activity on a dry weight per hour basis was about 1000 millimoles in the apical meristem and two youngest primordia, and declined to 150 millimoles in the mesophyll of the sixth leaf pair and to 80 millimoles in older leaves just expanded out of the bud”

✓ located verbatim in the source · abstract, characters 179–453 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Phosphofructokinase activity declined to 150 millimoles per dry weight per hour in the mesophyll of the sixth leaf pair.

Taxon
Dianthus chinensisDianthus chinensise0
Organ / stage
mesophyll of the sixth leaf paire1
Outcome
activitye1
Direction
declinede1
Value
150 millimolese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus chinensis
Dianthus chinensis · Taxon · picture from its Wikimedia Commons categoryGitartha.bordoloi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Phosphofructokinase (EC 2.7.1.11) activity in the apical meristem, leaf primordia, and leaf tissues (epidermis, mesophyll, xylem, phloem) of Dianthus chinensis L. was determined”

✓ located verbatim in the source · abstract, characters 0–177 · show in text ↑
e1

“These data show that activity on a dry weight per hour basis was about 1000 millimoles in the apical meristem and two youngest primordia, and declined to 150 millimoles in the mesophyll of the sixth leaf pair and to 80 millimoles in older leaves just expanded out of the bud”

✓ located verbatim in the source · abstract, characters 179–453 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Phosphofructokinase activity declined to 80 millimoles per dry weight per hour in older leaves just expanded out of the bud.

Taxon
Dianthus chinensisDianthus chinensise0
Organ / stage
older leaves just expanded out of the bude1
Outcome
activitye1
Direction
declinede1
Value
80 millimolese1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus chinensis
Dianthus chinensis · Taxon · picture from its Wikimedia Commons categoryGitartha.bordoloi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Phosphofructokinase (EC 2.7.1.11) activity in the apical meristem, leaf primordia, and leaf tissues (epidermis, mesophyll, xylem, phloem) of Dianthus chinensis L. was determined”

✓ located verbatim in the source · abstract, characters 0–177 · show in text ↑
e1

“These data show that activity on a dry weight per hour basis was about 1000 millimoles in the apical meristem and two youngest primordia, and declined to 150 millimoles in the mesophyll of the sixth leaf pair and to 80 millimoles in older leaves just expanded out of the bud”

✓ located verbatim in the source · abstract, characters 179–453 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

On a cell-per-hour basis, phosphofructokinase activity increased from 75 femtomoles in apical meristem and primordia cells to 1.5 picomoles in mesophyll cells of the sixth leaf pair.

Taxon
Dianthus chinensisDianthus chinensise0
Organ / stage
apical meristem and leaf primordiae2
Outcome
activitye2
Direction
increasede2
Value
75 femtomolese2
Qualifier
to 1.5 picomoles in the mesophyll of the sixth leaf paire2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus chinensis
Dianthus chinensis · Taxon · picture from its Wikimedia Commons categoryGitartha.bordoloi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Phosphofructokinase (EC 2.7.1.11) activity in the apical meristem, leaf primordia, and leaf tissues (epidermis, mesophyll, xylem, phloem) of Dianthus chinensis L. was determined”

✓ located verbatim in the source · abstract, characters 0–177 · show in text ↑
e2

“On a cell per hour basis, the activity increased from 75 femtomoles in cells of the apical meristem and leaf primordia to 1.5 picomoles in the mesophyll of the sixth leaf pair”

✓ located verbatim in the source · abstract, characters 455–630 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

On a cell-volume-per-hour basis, phosphofructokinase activity decreased along leaf development.

Taxon
Dianthus chinensisDianthus chinensise0
Organ / stage
leaf developmente4
Outcome
activitye0
Direction
decreaseinferrede3
Qualifier
on a cell volume (cubic micrometers) per hour basise3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus chinensis
Dianthus chinensis · Taxon · picture from its Wikimedia Commons categoryGitartha.bordoloi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Phosphofructokinase (EC 2.7.1.11) activity in the apical meristem, leaf primordia, and leaf tissues (epidermis, mesophyll, xylem, phloem) of Dianthus chinensis L. was determined”

✓ located verbatim in the source · abstract, characters 0–177 · show in text ↑
e3

“However, on a cell volume (cubic micrometers) per hour basis, the opposite was true”

✓ located verbatim in the source · abstract, characters 632–715 · show in text ↑
e4

“The results indicate that (a) on a dry weight or unit cell volume basis the capacity for glycolysis declines during leaf development”

✓ located verbatim in the source · abstract, characters 717–849 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The capacity for glycolysis declines during leaf development on a dry weight or unit cell volume basis.

Taxon
Dianthus chinensisDianthus chinensise0
Organ / stage
leaf developmente4
Outcome
capacity for glycolysise4
Direction
declinese4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus chinensis
Dianthus chinensis · Taxon · picture from its Wikimedia Commons categoryGitartha.bordoloi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Phosphofructokinase (EC 2.7.1.11) activity in the apical meristem, leaf primordia, and leaf tissues (epidermis, mesophyll, xylem, phloem) of Dianthus chinensis L. was determined”

✓ located verbatim in the source · abstract, characters 0–177 · show in text ↑
e4

“The results indicate that (a) on a dry weight or unit cell volume basis the capacity for glycolysis declines during leaf development”

✓ located verbatim in the source · abstract, characters 717–849 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Incipient primordia in the apical meristem do not show phosphofructokinase activity greater than adjacent meristem areas.

Taxon
Dianthus chinensisDianthus chinensise0
Organ / stage
incipient primordiae4
Outcome
phosphofructokinase activitye4
Direction
greatere4
Comparator
adjacent areas of the meristeme4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus chinensis
Dianthus chinensis · Taxon · picture from its Wikimedia Commons categoryGitartha.bordoloi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Phosphofructokinase (EC 2.7.1.11) activity in the apical meristem, leaf primordia, and leaf tissues (epidermis, mesophyll, xylem, phloem) of Dianthus chinensis L. was determined”

✓ located verbatim in the source · abstract, characters 0–177 · show in text ↑
e4

“within the apical meristem, incipient primordia are not associated with phosphofructokinase activity greater than adjacent areas of the meristem”

✓ located verbatim in the source · abstract, characters 863–1007 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The capacity of the glycolytic pathway is greater than that of the pentose phosphate pathway in the apical meristem.

Taxon
Dianthus chinensisDianthus chinensise0
Organ / stage
apical meristeme5
Outcome
capacity of the glycolytic pathwaye5
Direction
greatere5
Comparator
the pentose phosphate pathwaye5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Dianthus chinensis
Dianthus chinensis · Taxon · picture from its Wikimedia Commons categoryGitartha.bordoloi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Phosphofructokinase (EC 2.7.1.11) activity in the apical meristem, leaf primordia, and leaf tissues (epidermis, mesophyll, xylem, phloem) of Dianthus chinensis L. was determined”

✓ located verbatim in the source · abstract, characters 0–177 · show in text ↑
e5

“the capacity of the glycolytic pathway is greater than the pentose phosphate pathway in the apical meristem”

✓ located verbatim in the source · abstract, characters 1156–1263 · show in text ↑