Abstract
Summary The necrotrophic fungus Rhizoctonia cerealis is the major pathogen causing sharp eyespot disease in wheat ( Triticum aestivum ). Nucleotide‐binding leucine‐rich repeat ( NB ‐ LRR ) proteins often mediate plant disease resistance to biotrophic pathogens. Little is known about the role of NB ‐ LRR genes involved in wheat response to R. cerealis . In this study, a wheat NB ‐ LRR gene, named Ta RCR 1 , was identified in response to R. cerealis infection using Artificial Neural Network analysis based on comparative transcriptomics and its defence role was characterized. The transcriptional level of Ta RCR 1 was enhanced after R. cerealis inoculation and associated with the resistance level of wheat. Ta RCR 1 was located on wheat chromosome 3 BS and encoded an NB ‐ LRR protein that was consisting of a coiled‐coil domain, an NB ‐ ARC domain and 13 imperfect leucine‐rich repeats. Ta RCR 1 was localized in both the cytoplasm and the nucleus. Silencing of Ta RCR 1 impaired wheat resistance to R. cerealis , whereas Ta RCR 1 overexpression significantly increased the resistance in transgenic wheat. Ta RCR 1 regulated certain reactive oxygen species ( ROS )‐scavenging and production, and defence‐related genes, and peroxidase activity. Furthermore, H 2 O 2 pretreatment for 12‐h elevated expression levels of Ta RCR 1 and the above defence‐related genes, whereas treatment with a peroxidase inhibitor for 12 h reduced the resistance of Ta RCR 1‐ overexpressing transgenic plants and expression levels of these defence‐related genes. Taken together, Ta RCR 1 positively contributes to defence response to R. cerealis through maintaining ROS homoeostasis and regulating the expression of defence‐related genes.
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Claims 12
Claim · model-written summary
Wheat Ta RCR 1 was identified in response to R. cerealis infection.
- Taxon
- wheat
Triticum aestivumt0 - Gene
- Ta RCR 1e0
- Outcome
- identified in response to R. cerealis infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a wheat NB ‐ LRR gene, named Ta RCR 1 , was identified in response to R. cerealis infection using Artificial Neural Network analysis based on comparative transcriptomics”
t0“wheat”
Claim · model-written summary
Wheat transcriptional level of Ta RCR 1 was enhanced.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- R. cerealis inoculatione0
- Interactor
- R. cerealis
Ceratobasidium cerealee0pathogen - Gene
- transcriptional level of Ta RCR 1e0
- Outcome
- enhancede0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcriptional level of Ta RCR 1 was enhanced after R. cerealis inoculation and associated with the resistance level of wheat.”
t0“wheat”
Claim · model-written summary
Wheat Ta RCR 1 was located on wheat chromosome 3 BS.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Ta RCR 1e0
- Outcome
- located on wheat chromosome 3 BSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ta RCR 1 was located on wheat chromosome 3 BS”
t0“wheat”
Claim · model-written summary
Wheat NB ‐ LRR protein was coiled‐coil domain, an NB ‐ ARC domain and 13 imperfect leucine‐rich repeats.
- Taxon
- wheatTriticum aestivumt0
- Gene
- NB ‐ LRR proteine0
- Outcome
- coiled‐coil domain, an NB ‐ ARC domain and 13 imperfect leucine‐rich repeatse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“encoded an NB ‐ LRR protein that was consisting of a coiled‐coil domain, an NB ‐ ARC domain and 13 imperfect leucine‐rich repeats”
t0“wheat”
Claim · model-written summary
Wheat Ta RCR 1 was localized in both the cytoplasm and the nucleus.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Ta RCR 1e0
- Outcome
- localized in both the cytoplasm and the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ta RCR 1 was localized in both the cytoplasm and the nucleus”
t0“wheat”
Claim · model-written summary
Silencing Ta RCR 1 impaired wheat resistance to R. cerealis.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Silencing of Ta RCR 1e0
- Interactor
- R. cerealisCeratobasidium cerealee0pathogen
- Gene
- Ta RCR 1e0
- Outcome
- wheat resistance to R. cerealise0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of Ta RCR 1 impaired wheat resistance to R. cerealis”
Claim · model-written summary
Ta RCR 1 overexpression significantly increased resistance in transgenic wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Ta RCR 1 overexpressione0
- Gene
- Ta RCR 1e0
- Outcome
- resistancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ta RCR 1 overexpression significantly increased the resistance in transgenic wheat”
Claim · model-written summary
Ta RCR 1 regulated ROS scavenging and production, defence genes, and peroxidase activity.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Ta RCR 1e0
- Outcome
- reactive oxygen species ( ROS )‐scavenging and production, and defence‐related genes, and peroxidase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ta RCR 1 regulated certain reactive oxygen species ( ROS )‐scavenging and production, and defence‐related genes, and peroxidase activity.”
t0“wheat”
Claim · model-written summary
Twelve-hour H 2 O 2 pretreatment elevated Ta RCR 1 and defence gene expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- H 2 O 2 pretreatment for 12‐he0
- Gene
- Ta RCR 1e0
- Outcome
- expression levels of Ta RCR 1 and the above defence‐related genese0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H 2 O 2 pretreatment for 12‐h elevated expression levels of Ta RCR 1 and the above defence‐related genes”
t0“wheat”
Claim · model-written summary
Peroxidase inhibitor treatment reduced resistance in Ta RCR 1 overexpressing plants.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- treatment with a peroxidase inhibitor for 12 he0
- Gene
- Ta RCR 1e0
- Outcome
- resistancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with a peroxidase inhibitor for 12 h reduced the resistance of Ta RCR 1‐ overexpressing transgenic plants and expression levels of these defence‐related genes”
t0“wheat”
Claim · model-written summary
Peroxidase inhibitor treatment reduced defence gene expression.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- treatment with a peroxidase inhibitor for 12 he0
- Outcome
- expression levels of these defence‐related genese0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with a peroxidase inhibitor for 12 h reduced the resistance of Ta RCR 1‐ overexpressing transgenic plants and expression levels of these defence‐related genes”
t0“wheat”
Claim · model-written summary
Ta RCR 1 contributes to wheat defence against R. cerealis through ROS homeostasis and defence gene regulation.
- Taxon
- wheatTriticum aestivumt0
- Interactor
- R. cerealisCeratobasidium cerealee0pathogen
- Gene
- Ta RCR 1e0
- Outcome
- defence response to R. cerealise0
- Qualifier
- through maintaining ROS homoeostasis and regulating the expression of defence‐related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ta RCR 1 positively contributes to defence response to R. cerealis through maintaining ROS homoeostasis and regulating the expression of defence‐related genes.”
t0“wheat”
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See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.